breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 16,804 T→C I34V (ATC→GTC)  hokC ← toxic membrane protein, small
RA 60,012 T→A D112V (GAT→GTT)  rluA ← dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 82,440 C→A A364S (GCG→TCG)  leuA ← 2‑isopropylmalate synthase
RA 109,644 A→G T456A (ACT→GCT)  secA → preprotein translocase subunit, ATPase
RA 115,331 C→T G132S (GGT→AGT)  hofC ← assembly protein in type IV pilin biogenesis, transmembrane protein
RA 155,698 A→G G168G (GGT→GGC yadV ← putative periplasmic pilin chaperone
RA 176,263 C→T A386V (GCG→GTG)  clcA → H(+)/Cl(‑) exchange transporter
RA 218,942 A→G V218A (GTT→GCT)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
RA 231,261 T→C L47P (CTG→CCG)  yafD → endo/exonuclease/phosphatase family protein
MC JC 257,908 Δ776 bp [crl] [crl]
RA 265,090 A→G A39A (GCT→GCC yafX ← CP4‑6 prophage; uncharacterized protein
RA 266,175 A→G Y127H (TAC→CAC)  yafY ← lipoprotein, inner membrane; degP regulator; CP4‑6 prophage
RA 304,834 A→G R124R (CGT→CGC ecpE ← ECP production pilus chaperone
RA 339,165 G→A D281N (GAC→AAC)  yahG → DUF1116 family protein
RA 355,718 T→C L266P (CTG→CCG)  codB → cytosine transporter
RA 380,022 (G)10→11 intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein
RA 409,847 T→C H58R (CAC→CGC)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 438,035 G→A T34I (ACA→ATA)  yajO ← 2‑carboxybenzaldehyde reductase
RA 474,819 A→G F115L (TTC→CTC)  tesB ← acyl‑CoA thioesterase 2
RA 483,817 A→G F196S (TTC→TCC)  acrB ← multidrug efflux system protein
RA 531,493 T→A K243N (AAA→AAT allS ← allD operon transcriptional activator
RA 561,299 A→G T535A (ACC→GCC)  sfmD → putative outer membrane export usher protein
RA 577,085 G→A intergenic (‑260/‑313) nmpC ← / → essD DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein
RA 611,255 C→T A414T (GCT→ACT)  fepA ← ferrienterobactin outer membrane transporter
RA 617,735 A→G E1193E (GAA→GAG entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 643,563 C→T L135L (CTG→CTA rnk ← regulator of nucleoside diphosphate kinase
RA 681,014 A→G E268G (GAA→GGA)  djlC → J domain‑containing HscC co‑chaperone; Hsc56
RA 706,802 A→G N237S (AAC→AGC)  glnS → glutamyl‑tRNA synthetase
RA 721,504 A→G V77A (GTG→GCG)  kdpE ← response regulator in two‑component regulatory system with KdpD
RA 741,429 A→G S376S (TCT→TCC dtpD ← dipeptide and tripeptide permease D
RA 767,750 C→T V589V (GTC→GTT mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 781,084 T→C noncoding (17/76 nt) valZ → tRNA‑Val
RA 807,115 T→C Q56R (CAA→CGA)  ybhC ← acyl‑CoA thioesterase, lipoprotein
RA 846,224 A→G G80G (GGT→GGC glnQ ← glutamine transporter subunit
RA 860,657 A→G R650R (CGT→CGC ybiW ← putative pyruvate formate lyase
RA 922,723 G→A intergenic (‑133/‑190) cspD ← / → clpS inhibitor of DNA replication, cold shock protein homolog/regulatory protein for ClpA substrate specificity
RA 939,609 C→T A61V (GCG→GTG)  serS → seryl‑tRNA synthetase
JC JC 944,912 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp intergenic (+16/+14) dmsC → / ← ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
RA 968,630 A→G K89E (AAG→GAG)  lpxK → lipid A 4'kinase
RA 1,034,488 C→T P412L (CCG→CTG)  hyaB → hydrogenase 1, large subunit
RA 1,097,864 A→G intergenic (‑212/‑22) serX ← / → ghrA tRNA‑Ser/glyoxylate/hydroxypyruvate reductase A
RA 1,114,450 A→G R347R (CGT→CGC mdtG ← putative drug efflux system protein
RA 1,125,128 C→T G67S (GGT→AGT)  mdtH ← multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
RA 1,154,782 A→G E224G (GAA→GGA)  yceG → septation protein, ampicillin sensitivity
RA 1,246,015 T→C A454A (GCA→GCG treA ← periplasmic trehalase
RA 1,258,521 (T)7→6 intergenic (‑8/‑270) pth ← / → ychH peptidyl‑tRNA hydrolase/DUF2583 family putative inner membrane protein
RA 1,293,491 C→T intergenic (‑569/‑36) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,312,615 G→A S92S (AGC→AGT yciB ← IspA family inner membrane protein
RA 1,314,744 T→C T161A (ACT→GCT)  yciE ← putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,317,160 A→G P21P (CCT→CCC trpA ← tryptophan synthase, alpha subunit
RA 1,398,201 G→A P141L (CCG→CTG)  uspE ← stress‑induced protein
RA 1,400,623 C→T G384D (GGC→GAC)  abgT ← p‑aminobenzoyl‑glutamate transporter; membrane protein
RA 1,409,591 G→A T27T (ACG→ACA dbpA → ATP‑dependent RNA helicase, specific for 23S rRNA
RA 1,429,098 T→C V17A (GTA→GCA)  stfR → Rac prophage; putative tail fiber protein
RA 1,446,241 A→G intergenic (+35/+137) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB
RA 1,466,123 4 bp→TGCT pseudogene (732‑735/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,129 T→C pseudogene (738/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,141 A→G pseudogene (750/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,517,218 C→T intergenic (‑24/‑171) yncL ← / → ydcX stress‑induced small inner membrane enterobacterial protein/DUF2566 family protein
RA 1,597,313 (C)7→8 pseudogene (675/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,692,029 T→C T275A (ACG→GCG)  uidC ← putative outer membrane porin for beta‑glucuronides porin protein
RA 1,715,182 (C)5→6 coding (708/864 nt) pdxY ← pyridoxamine kinase
RA 1,762,052 T→C N154S (AAC→AGC)  sufC ← SufBCD Fe‑S cluster assembly scaffold protein, ATP‑binding protein
RA 1,768,998 T→C intergenic (‑313/‑76) ydiJ ← / → ydiK putative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein
RA 1,863,313 T→C A181A (GCT→GCC gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,888,259 C→T A496A (GCG→GCA fadD ← acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
RA 1,894,752 T→C intergenic (‑21/‑53) yoaH ← / → pabB UPF0181 family protein/aminodeoxychorismate synthase, subunit I
RA 1,922,198 A→G E26E (GAA→GAG yebW → uncharacterized protein
RA 1,928,306 G→A V178V (GTC→GTT ptrB ← protease II
RA 1,931,728 G→A R283H (CGT→CAT)  purT → phosphoribosylglycinamide formyltransferase 2
RA 1,947,662 T→C T164A (ACC→GCC)  yebC ← UPF0082 family protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,000,046 G→A R114C (CGC→TGC)  fliY ← cystine transporter subunit
RA 2,025,889 G→A A127T (GCC→ACC)  yedP → putative mannosyl‑3‑phosphoglycerate phosphatase
RA 2,026,299 G→A G263G (GGG→GGA yedP → putative mannosyl‑3‑phosphoglycerate phosphatase
RA 2,096,784 T→C T57T (ACT→ACC hisI → phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase
RA 2,117,002 T→C T42T (ACA→ACG wcaL ← putative glycosyl transferase
RA 2,159,008 T→C V208A (GTC→GCC)  mdtC → multidrug efflux system, subunit C
RA 2,164,440 G→A P55P (CCG→CCA baeR → response regulator in two‑component regulatory system with BaeS
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,224,908 C→T intergenic (‑38/+136) pbpG ← / ← yohC D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
RA 2,241,409 T→C Q87R (CAG→CGG)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
RA 2,273,106 C→T T248I (ACC→ATC)  yejA → microcin C ABC transporter periplasmic binding protein
RA 2,355,127 T→C V288A (GTA→GCA)  glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
RA 2,359,249 T→C T302A (ACC→GCC)  rhmT ← putative L‑rhamnonate transporter
RA 2,406,631 G→A A4V (GCA→GTA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,453,901 G→A pseudogene (1101/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
RA 2,458,447 T→C H238R (CAC→CGC)  fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,467,446 A→G K301R (AAG→AGG)  intS → CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
RA 2,476,595 A→G pseudogene (12/15 nt) pawZ → tRNA‑OTHER
RA 2,555,334 G→A A132V (GCC→GTC)  eutK ← putative ethanol utilization carboxysome structural protein
RA 2,579,841 T→C L69P (CTG→CCG)  tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,598,854 A→G S4S (TCT→TCC bamC ← BamABCDE complex OM biogenesis lipoprotein
RA 2,609,458 T→C R324R (CGT→CGC hyfG → hydrogenase 4, subunit
RA 2,611,827 T→C V124A (GTG→GCG)  hyfJ → putative processing element hydrogenase 4
JC 2,636,790 (TTC)3→2 coding (565‑567/1473 nt) der ← GTPase; multicopy suppressor of ftsJ
RA 2,647,150 G→A A59V (GCT→GTT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
RA 2,647,909 T→C K1460R (AAA→AGA)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,647,979 T→C T1437A (ACG→GCG)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,662,523 A→G intergenic (‑392/+60) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,767,140 T→C L53L (TTG→CTG)  rnlB → CP4‑57 prophage; uncharacterized protein
RA 2,777,617 T→C A55A (GCT→GCC ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,780,330 G→A G799G (GGC→GGT ypjA ← adhesin‑like autotransporter
RA 2,854,255 T→C R334R (CGT→CGC hypE → carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein
RA 2,892,689 T→C G11G (GGT→GGC ygcN → putative oxidoreductase
RA 2,910,220 C→T G156S (GGC→AGC)  mazG ← nucleoside triphosphate pyrophosphohydrolase
RA 2,912,885 A→G I256T (ATC→ACC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,914,809 A→G A64A (GCT→GCC rlmD ← 23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
RA 2,960,839 A→G R530R (CGT→CGC recC ← exonuclease V (RecBCD complex), gamma chain
RA 2,985,465 A→G V43A (GTT→GCT)  yqeF ← short chain acyltransferase
RA 3,011,590 G→A V44I (GTC→ATC)  yqeA → putative amino acid kinase
RA 3,053,637 T→C I402V (ATC→GTC)  pepP ← proline aminopeptidase P II
RA 3,086,618 A→G intergenic (+216/‑88) yqgC → / → metK uncharacterized protein/S‑adenosylmethionine synthetase
RA 3,111,537 T→C pseudogene (554/963 nt) yghE ← pseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)
RA 3,136,817 T→C Q569R (CAA→CGA)  gss ← glutathionylspermidine amidase and glutathionylspermidine synthetase
RA 3,272,271 Δ1 bp coding (481/885 nt) garR ← tartronate semialdehyde reductase
RA 3,276,550 C→T G423G (GGC→GGT garD → D‑galactarate dehydrogenase
RA 3,280,801 T→C pseudogene (101/375 nt) agaW → pseudogene, N‑acetylgalactosamine‑specific enzyme IIC component of PTS;enzyme; Central intermediary metabolism: Amino sugars; PTS system N‑acetylgalactosameine‑specific IIC component 2
RA 3,358,491 A→G H1256R (CAC→CGC)  gltB → glutamate synthase, large subunit
RA 3,376,403 T→C D125G (GAC→GGC)  sspB ← ClpXP protease specificity enhancing factor
RA 3,377,215 A→G L69P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
RA 3,386,639 T→C N517S (AAT→AGT)  aaeB ← p‑hydroxybenzoic acid efflux system component
RA 3,429,355 G→A W40* (TGG→TGA yrdA → bacterial transferase hexapeptide domain protein
RA 3,432,487 A→G Y358Y (TAT→TAC smf ← DNA recombination‑mediator A family protein
RA 3,440,116 T→C D305G (GAC→GGC)  rpoA ← RNA polymerase, alpha subunit
RA 3,449,100 T→C T24A (ACG→GCG)  rplP ← 50S ribosomal subunit protein L16
RA 3,462,560 A→G T73A (ACG→GCG)  gspJ → putative general secretory pathway component, cryptic
RA 3,502,861 G→A P174P (CCG→CCA frlC → fructoselysine 3‑epimerase
RA 3,515,565 A→G Y117H (TAC→CAC)  dam ← DNA adenine methyltransferase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,571,794 T→C N570S (AAC→AGC)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,605,363 A→G M83T (ATG→ACG)  yhhM ← DUF2500 family protein
RA 3,612,540 T→C W226R (TGG→CGG)  yhhT → UPF0118 family putative transporter
RA 3,668,091 T→C E366E (GAA→GAG yhjA ← putative cytochrome C peroxidase
RA 3,691,676 T→C Y311C (TAC→TGC)  bcsB ← regulator of cellulose synthase, cyclic di‑GMP binding
RA 3,751,508 T→C T121A (ACC→GCC)  yiaT ← putative outer membrane protein
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,928,879 A→G V242A (GTG→GCG)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,936,721 T→C G148G (GGT→GGC rbsB → D‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor
RA 3,937,495 A→G S68G (AGC→GGC)  rbsK → ribokinase
RA 4,003,303 A→G R292R (CGT→CGC rarD ← putative chloramphenical resistance permease
RA 4,099,540 A→G V329A (GTG→GCG)  rhaT ← L‑rhamnose:proton symporter
RA 4,128,316 A→G V27A (GTT→GCT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,140,495 T→C D576G (GAT→GGT)  frwA ← putative PTS enzyme: Hpr, enzyme I and II components
RA 4,185,098 A→G Y1285C (TAC→TGC)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,366,223 A→G F184S (TTC→TCC)  dcuA ← C4‑dicarboxylate antiporter
RA 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,401,589 C→T D306D (GAC→GAT hflX → GTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
RA 4,460,207 T→C D53G (GAC→GGC)  nrdG ← anaerobic ribonucleotide reductase activating protein
RA 4,469,762 T→C V713A (GTG→GCG)  mgtA → magnesium transporter
RA 4,513,835 T→C E158E (GAA→GAG fecB ← ferric citrate ABC transporter periplasmic binding protein
RA 4,516,435 G→A S81S (AGC→AGT fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
RA 4,552,990 C→T C30C (TGC→TGT uxuB → D‑mannonate oxidoreductase, NAD‑dependent
RA 4,575,814 A→G pseudogene (1896/2937 nt) yjiV → pseudogene; conserved hypothetical protein
RA 4,619,179 A→G V317V (GTA→GTG deoA → thymidine phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1466145–1466203 1466509 307–365 52 [51] [51] 52 ydbA pseudogene, autotransporter homolog; interrupted by IS2 and IS30
* * ÷ NC_000913 1531991–1532124 1532844–1532728 605–854 52 [49] [51] 52 ydcC H repeat‑associated putative transposase
* * ÷ NC_000913 3423733–3424234 3424564–3424238 5–832 53 [51] [51] 52 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3762346–3764074 3765243–3764077 4–2898 52 [51] [51] 52 rhsA Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
* * ÷ NC_000913 3944135–3945567 3946562–3946229 663–2428 52 [51] [51] 52 rrlC 23S ribosomal RNA of rrnC operon
* * ÷ NC_000913 4153383 4153636 254 52 [50] [51] 52 ppc/argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =36 (0.390)57 (0.690) 52/266 1.2 68.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 21 (0.250)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780536 (0.390)61 (0.730) 50/266 1.3 69.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 21 (0.250)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)98 (1.080) 79/290 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein