Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	758,260	A→G	D191G (GAC→GGC)	sdhB →	succinate dehydrogenase, FeS subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,260	0	A	G	100.0%	152.9 / NA	42	D191G (GAC→GGC)	sdhB	succinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/19); total (23/19)

CGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCG  >  NC_000913/758117‑758402

cGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAgccgc                                                                                                                                         <  2:293040/151‑1 (MQ=255)

   cGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTc                                                                                                                                      >  1:661214/1‑151 (MQ=255)

          tATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGt                                                                                                                                <  2:591542/150‑1 (MQ=255)

                 gTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTATCGGCATATCGTTTCCTGATTTATAGCCGTGATACCGAGCCTGGCAGCCGCCTCGACGGTTTGAgcg                                                                                                                         >  1:516696/1‑148 (MQ=255)

                   aTTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGAt                                                                                                                       >  1:219844/1‑150 (MQ=255)

                              aTGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCAGCGt                                                                                                            >  1:49098/1‑150 (MQ=255)

                                   gttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCCCGACGGTTTGAGTGATGCATTCAGCGGATTcc                                                                                                       >  1:445608/1‑150 (MQ=255)

                                     tCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGc                                                                                                     >  2:539563/1‑150 (MQ=255)

                                         ccTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTc                                                                                                 <  1:484503/150‑1 (MQ=255)

                                          ctctTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcac                                                                                               >  1:68287/1‑151 (MQ=255)

                                             ttGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAg                                                                                             >  2:533200/1‑150 (MQ=255)

                                              tGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTTGCGGCATATCGTTTCCTGATTGATAACCGTGGTACCGAGACTGGCAGCCGCCTCGACGGGTTGGGTGATGCCTTCAACGTATTCCGCTGGca                                                                                                >  2:643346/1‑146 (MQ=255)

                                              tGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGc                                                                                            <  1:388081/150‑1 (MQ=255)

                                                tCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGcat                                                                                          <  1:52727/150‑1 (MQ=255)

                                                   gTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGGGATGCATTCAGCGTATTCCGCTGTCACAGcatcat                                                                                       >  2:175894/1‑150 (MQ=255)

                                                        tCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACt                                                                                  <  2:412247/150‑1 (MQ=255)

                                                        tCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACt                                                                                  <  2:385376/150‑1 (MQ=255)

                                                          tggtggAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAACCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCCGCGTATTCCGCTGTCACAGCATCATGaa                                                                                    >  1:285642/1‑146 (MQ=255)

                                                           ggtggAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCg                                                                               <  2:144033/150‑1 (MQ=255)

                                                               gAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTc                                                                             >  2:441269/1‑148 (MQ=255)

                                                                       aTAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTcc                                                                  <  1:10800/151‑1 (MQ=255)

                                                                          aGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCCGATTGCTAGCCGTGATACCGAAACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCAGCGTATTTCGCTGTCACAGCATCATTAACTGCGTCAGTGTATGTCCg                                                                 >  2:313959/1‑149 (MQ=255)

                                                                             ttATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAgg                                                             <  2:649439/150‑1 (MQ=255)

                                                                              tATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAggg                                                            <  1:648140/150‑1 (MQ=255)

                                                                               aTCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAgggg                                                           <  1:486315/150‑1 (MQ=255)

                                                                                      cGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAAccc                                                   >  1:216647/1‑151 (MQ=255)

                                                                                       ggCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCg                                                  >  2:604385/1‑151 (MQ=255)

                                                                                         cAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAc                                                >  1:158832/1‑151 (MQ=255)

                                                                                               tGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgcgc                                            <  1:248504/149‑1 (MQ=255)

                                                                                                 ttAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGcc                                         >  1:537156/1‑150 (MQ=255)

                                                                                                      ggCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGc                                   >  1:454999/1‑151 (MQ=255)

                                                                                                        cATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGcc                                  >  2:143420/1‑150 (MQ=255)

                                                                                                         atatCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCat                                <  1:473241/151‑1 (MQ=255)

                                                                                                           atCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCatat                              >  1:52142/1‑151 (MQ=255)

                                                                                                              gTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCaa                           <  1:276953/151‑1 (MQ=255)

                                                                                                                 tCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGt                         <  2:560631/150‑1 (MQ=255)

                                                                                                                 tCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTc                        >  1:649316/1‑151 (MQ=255)

                                                                                                                  ccTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCg                       >  1:70401/1‑151 (MQ=255)

                                                                                                                  ccTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCg                       >  1:425647/1‑151 (MQ=255)

                                                                                                                        tgatAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGAtgtt                  <  2:636152/150‑1 (MQ=255)

                                                                                                                                gTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACg         <  2:558801/151‑1 (MQ=255)

                                                                                                                                       cGATACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCATCGTAATGCg  <  1:643508/151‑1 (MQ=255)

CGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCG  >  NC_000913/758117‑758402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A