Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	817,261	A→G	E73G (GAG→GGG)	moaA →	molybdopterin biosynthesis protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	817,261	0	A	G	100.0%	118.5 / NA	35	E73G (GAG→GGG)	moaA	molybdopterin biosynthesis protein A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/16); total (19/16)

AACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATT  >  NC_000913/817116‑817403

aaCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGt                                                                                                                                           <  1:2086/151‑1 (MQ=255)

 aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTc                                                                                                                                          <  2:575623/151‑1 (MQ=255)

    ttCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCtt                                                                                                                                        <  2:463016/150‑1 (MQ=255)

            aCCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCcgcg                                                                                                                               >  1:290613/1‑151 (MQ=255)

               tACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACt                                                                                                                            >  1:306814/1‑151 (MQ=255)

                      tgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCg                                                                                                                      >  2:276889/1‑150 (MQ=255)

                          ggATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATatc                                                                                                                 <  1:655254/151‑1 (MQ=255)

                            aTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATatcat                                                                                                               >  2:599388/1‑151 (MQ=255)

                                  aCAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGTTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATcgcc                                                                                                           <  2:8315/149‑1 (MQ=255)

                                  aCAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATcgccgc                                                                                                         <  2:283912/151‑1 (MQ=255)

                                   cAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCt                                                                                                        >  1:414422/1‑151 (MQ=255)

                                      aCCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCtgtg                                                                                                     <  1:6558/151‑1 (MQ=255)

                                        cGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCg                                                                                                   <  1:205543/151‑1 (MQ=255)

                                          aGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCggg                                                                                                 >  2:677596/1‑151 (MQ=255)

                                            cggcgTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGa                                                                                                >  2:437280/1‑150 (MQ=255)

                                            cggcgTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGa                                                                                                >  1:496466/1‑150 (MQ=255)

                                                 tCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAac                                                                                            >  2:484558/1‑149 (MQ=255)

                                                   aCCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAacgacg                                                                                        >  2:392373/1‑151 (MQ=255)

                                                   aCCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAacgacg                                                                                        <  2:125049/151‑1 (MQ=255)

                                                         aaaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATcc                                                                                  <  1:152080/151‑1 (MQ=255)

                                                          aaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCg                                                                                 >  2:215716/1‑151 (MQ=255)

                                                               tttCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGcc                                                                               >  2:181770/1‑148 (MQ=255)

                                                                                  ttCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTAt                                                                                    >  2:299206/1‑124 (MQ=255)

                                                                                  ttCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTAt                                                                                    <  1:299213/124‑1 (MQ=255)

                                                                                  ttCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATgg                                                         >  2:69973/1‑151 (MQ=255)

                                                                                       cGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTAcc                                                    >  1:535210/1‑151 (MQ=255)

                                                                                          gTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTc                                                 <  2:129757/151‑1 (MQ=255)

                                                                                            tACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTg                                               >  1:143295/1‑151 (MQ=255)

                                                                                                    ccTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGa                                       <  1:177599/151‑1 (MQ=255)

                                                                                                     cTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAAcgc                                         <  1:141329/148‑1 (MQ=255)

                                                                                                      ttCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGAtg                                     >  1:206621/1‑151 (MQ=255)

                                                                                                          ccAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGc                                 >  1:74245/1‑151 (MQ=255)

                                                                                                                     aCCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGgcgc                       <  2:551467/150‑1 (MQ=255)

                                                                                                                          aaaaGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCg                 <  1:294606/151‑1 (MQ=255)

                                                                                                                                        cAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCAt   >  1:47485/1‑151 (MQ=255)

                                                                                                                                         aggaggaggGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCAtt  >  2:385480/1‑151 (MQ=255)

AACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATT  >  NC_000913/817116‑817403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A