Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,744	A→G	S240S (TCT→TCC)	glnQ ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,744	0	A	G	100.0%	151.4 / NA	39	S240S (TCT→TCC)	glnQ	glutamine transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/24); total (15/24)

TGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTC  >  NC_000913/845610‑845888

tGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCaaa                                                                                                                                    >  2:349920/1‑150 (MQ=255)

  aCAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAAt                                                                                                                                 <  1:626705/151‑1 (MQ=255)

         gCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGc                                                                                                                           <  2:330361/150‑1 (MQ=255)

         gCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCa                                                                                                                          <  2:24021/151‑1 (MQ=255)

            ggCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAgc                                                                                                                       >  2:378099/1‑151 (MQ=255)

                 ttttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTg                                                                                                                   <  1:631817/150‑1 (MQ=255)

                     gCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTc                                                                                                               <  1:224195/150‑1 (MQ=255)

                          aTGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCggg                                                                                                         >  1:61388/1‑151 (MQ=255)

                            gcggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCggggt                                                                                                       >  1:574588/1‑149 (MQ=255)

                            gcggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGt                                                                                                        <  2:85835/150‑1 (MQ=255)

                              ggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCt                                                                                                     <  2:415534/151‑1 (MQ=255)

                                     aCGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCaa                                                                                              <  2:253566/151‑1 (MQ=255)

                                     aCGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCa                                                                                               >  1:605482/1‑150 (MQ=255)

                                            aTCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTg                                                                                       <  1:332700/150‑1 (MQ=255)

                                               cGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCgg                                                                                    >  1:561156/1‑150 (MQ=255)

                                                 tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGAtt                                                                                 >  2:71375/1‑151 (MQ=255)

                                                       aaGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATc                                                                           <  2:462526/151‑1 (MQ=255)

                                                       aaGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATc                                                                           <  1:238338/151‑1 (MQ=255)

                                                                cAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTTTTGGTCAA‑CACCTGCGGATTGCCATCTTCCGCaa                                                                   >  1:223852/1‑150 (MQ=255)

                                                                 aaTTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATc                                                                 <  1:151085/151‑1 (MQ=255)

                                                                    tttAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCCTCGGATTGCCATCTTCCGCCATCCgg                                                              >  2:303195/1‑151 (MQ=255)

                                                                          aaGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCttt                                                         <  2:451626/150‑1 (MQ=255)

                                                                          aaGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCttt                                                         >  1:521258/1‑150 (MQ=255)

                                                                                    atatCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGa                                              <  1:41917/151‑1 (MQ=255)

                                                                                     tatCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCCGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATaaa                                                >  2:351632/1‑148 (MQ=255)

                                                                                      atCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGAt                                             <  1:482243/150‑1 (MQ=255)

                                                                                      atCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGAt                                             <  1:279301/150‑1 (MQ=255)

                                                                                        ccTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCa                                           <  2:76460/150‑1 (MQ=255)

                                                                                          tGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGGTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGcc                                        >  2:491267/1‑151 (MQ=255)

                                                                                            tAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGGTCCTGGTCAACCACCT‑CGGGTTGACCTCCTCCGCCATCCGGCCTTTGTCGATAAAGATCAGcccc                                      >  2:453170/1‑149 (MQ=255)

                                                                                               gCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGaa                                   <  2:224936/151‑1 (MQ=255)

                                                                                               gCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGACTTTGTCGATAAAGATCAGCCGCGaa                                   <  2:421348/151‑1 (MQ=255)

                                                                                                 cTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTTTCGATAAAGATCAGcc                                        >  2:258863/1‑144 (MQ=255)

                                                                                                      aaGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CAACTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACtt                            >  1:393773/1‑151 (MQ=255)

                                                                                                                cATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCaaa                  <  2:216334/151‑1 (MQ=255)

                                                                                                                 aTCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCaaaa                 <  2:32950/151‑1 (MQ=255)

                                                                                                                   cAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAAcc               <  1:71377/151‑1 (MQ=255)

                                                                                                                   cAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAAc                <  1:654944/150‑1 (MQ=255)

                                                                                                                                 tattaGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTc  <  2:220924/150‑1 (MQ=255)

TGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTC  >  NC_000913/845610‑845888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A