Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,013,706 T→C L150L (TTG→CTG)  pqiB → paraquat‑inducible, SoxRS‑regulated MCE domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,013,7060TC100.0% 165.5 / NA 48L150L (TTG→CTG) pqiBparaquat‑inducible, SoxRS‑regulated MCE domain protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

CGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAA  >  NC_000913/1013564‑1013843
                                                                                                                                              |                                                                                                                                         
cGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCg                                                                                                                                   >  2:204843/1‑151 (MQ=255)
cGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCg                                                                                                                                   <  1:457257/151‑1 (MQ=255)
   cTTTTGGGTGGTGAAACCGCAGATTGGTCGCGGAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCt                                                                                                                                >  1:237661/1‑151 (MQ=255)
       tGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATg                                                                                                                            >  1:599253/1‑151 (MQ=255)
        gggTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATg                                                                                                                            <  2:282121/150‑1 (MQ=255)
           tggtgAAACCGCAGGGTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGATGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATGAATACGATTTGCTGGACTAGCCACCGCTGGCCCCGCCTGATGCg                                                                                                                          >  2:253602/1‑149 (MQ=255)
           tggtgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGa                                                                                                                         >  2:87655/1‑150 (MQ=255)
            ggtgAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGaa                                                                                                                        >  1:57092/1‑150 (MQ=255)
                aaaCCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGt                                                                                                                    >  1:32149/1‑150 (MQ=255)
                    cGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCg                                                                                                               <  1:329122/151‑1 (MQ=255)
                          ttGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGACAGCAAAATGGATAACTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGAt                                                                                                         >  2:391788/1‑151 (MQ=255)
                           tGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGAt                                                                                                         <  1:47492/150‑1 (MQ=255)
                                 cgAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGAt                                                                                                  <  2:496904/151‑1 (MQ=255)
                                  gAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATa                                                                                                 <  1:84558/151‑1 (MQ=255)
                                    tgggATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGAATCCGTGTGATTCTCGATAg                                                                                                >  2:291510/2‑150 (MQ=255)
                                     gggATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGc                                                                                               >  1:390444/1‑150 (MQ=255)
                                        aTTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCaaaa                                                                                           <  2:178994/151‑1 (MQ=255)
                                        aTTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCaaaa                                                                                           <  2:5316/151‑1 (MQ=255)
                                         ttAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCaaaa                                                                                           >  2:145001/1‑150 (MQ=255)
                                             cGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGcc                                                                                      <  2:14765/151‑1 (MQ=255)
                                                   gggAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGc                                                                                  <  2:122732/149‑1 (MQ=255)
                                                     gAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGct                                                                              >  2:597599/1‑151 (MQ=255)
                                                                  tGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAgg                                                                     >  1:471253/1‑147 (MQ=255)
                                                                     aGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCg                                                              >  2:397559/1‑151 (MQ=255)
                                                                            atCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGt                                                       >  2:59614/1‑151 (MQ=255)
                                                                              cGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTc                                                     <  2:59612/151‑1 (MQ=255)
                                                                                    gCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTg                                                 <  1:407018/149‑1 (MQ=255)
                                                                                    gCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGc                                               >  1:276858/1‑151 (MQ=255)
                                                                                     cAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCt                                              <  2:27499/151‑1 (MQ=255)
                                                                                         cAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCg                                          <  2:660494/151‑1 (MQ=255)
                                                                                          aGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCgg                                         >  2:427860/1‑151 (MQ=255)
                                                                                                  aaGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTc                                 <  2:631179/151‑1 (MQ=255)
                                                                                                   aGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCg                                <  2:426502/151‑1 (MQ=255)
                                                                                                         caAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGaa                          <  2:230231/151‑1 (MQ=255)
                                                                                                               ggATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAg                     <  1:673972/150‑1 (MQ=255)
                                                                                                               ggATAAATACGATTTGCTGGACTCGCAACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGc                    <  1:284017/151‑1 (MQ=255)
                                                                                                                 aTAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCAc                  >  1:158748/1‑151 (MQ=255)
                                                                                                                  tAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCAcc                 >  2:83815/1‑151 (MQ=255)
                                                                                                                   aaaTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCt                <  1:2656/151‑1 (MQ=255)
                                                                                                                   aaaTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCt                <  1:99007/151‑1 (MQ=255)
                                                                                                                     aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc              <  1:511428/151‑1 (MQ=255)
                                                                                                                     aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc              <  1:135967/151‑1 (MQ=255)
                                                                                                                     aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc              >  1:66729/1‑151 (MQ=255)
                                                                                                                        cGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGAt           >  1:671209/1‑151 (MQ=255)
                                                                                                                           tttGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATac         >  2:313479/1‑150 (MQ=255)
                                                                                                                            ttGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATaca        <  1:19486/150‑1 (MQ=255)
                                                                                                                               cTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACaa     >  2:59336/1‑150 (MQ=255)
                                                                                                                                 ggACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACaaaaa  <  1:548803/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                         
CGTCTTTTGGGTGGTGAAACCGCAGATTGGTCGCGAAGGGATTAGCGGCCTGGGAACGCTGCTGTCTGGAGTTTATATCGAACTGCAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAA  >  NC_000913/1013564‑1013843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A