Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,013,807 A→G V183V (GTA→GTG)  pqiB → paraquat‑inducible, SoxRS‑regulated MCE domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,013,8070AG100.0% 161.7 / NA 46V183V (GTA→GTG) pqiBparaquat‑inducible, SoxRS‑regulated MCE domain protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

AAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGG  >  NC_000913/1013662‑1013954
                                                                                                                                                 |                                                                                                                                                   
aaGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTc                                                                                                                                                <  2:631179/151‑1 (MQ=255)
 aGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCg                                                                                                                                               <  2:426502/151‑1 (MQ=255)
       caAAATGGATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGaa                                                                                                                                         <  2:230231/151‑1 (MQ=255)
             ggATAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAg                                                                                                                                    <  1:673972/150‑1 (MQ=255)
             ggATAAATACGATTTGCTGGACTCGCAACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGc                                                                                                                                   <  1:284017/151‑1 (MQ=255)
               aTAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCAc                                                                                                                                 >  1:158748/1‑151 (MQ=255)
                tAAATACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCAcc                                                                                                                                >  2:83815/1‑151 (MQ=255)
                 aaaTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCt                                                                                                                               <  1:99007/151‑1 (MQ=255)
                 aaaTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCt                                                                                                                               <  1:2656/151‑1 (MQ=255)
                   aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc                                                                                                                             >  1:66729/1‑151 (MQ=255)
                   aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc                                                                                                                             <  1:511428/151‑1 (MQ=255)
                   aTACGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTc                                                                                                                             <  1:135967/151‑1 (MQ=255)
                      cGATTTGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGAt                                                                                                                          >  1:671209/1‑151 (MQ=255)
                         tttGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATac                                                                                                                        >  2:313479/1‑150 (MQ=255)
                          ttGCTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATaca                                                                                                                       <  1:19486/150‑1 (MQ=255)
                             cTGGACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACaa                                                                                                                    >  2:59336/1‑150 (MQ=255)
                               ggACTCGCCACCGCTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACaaaaa                                                                                                                 <  1:548803/151‑1 (MQ=255)
                                          cgctGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCTCCTTCGATACACAAAAACGCAATATCAg                                                                                                      <  1:451225/148‑1 (MQ=255)
                                           gctGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGc                                                                                                     >  2:632779/3‑151 (MQ=255)
                                             tGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCt                                                                                                    >  1:565571/1‑150 (MQ=255)
                                                 cccGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCa                                                                                                >  2:72175/1‑150 (MQ=255)
                                                   cGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCTCCTTCGATACACAAAAACGCAATATCAGCTATCAACt                                                                                             <  1:571217/151‑1 (MQ=255)
                                                      cTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACt                                                                                             <  1:319338/148‑1 (MQ=255)
                                                        gATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTtca                                                                                        <  2:191263/151‑1 (MQ=255)
                                                           gCGAAGGGTATCCGTGTGATTTTCGATAGAAAAAAAGCCGGGCACCGCTCGCCAGGGGATCCGGTGCGGTTCCGTGCCAATCGGGTGGGTTCGGTTGAAACAAGCACTTTGGATAAACAAAAAGGCAATATCAGCTATCAACTGTccat                                                                                       >  2:224177/1‑149 (MQ=255)
                                                           gCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTtcatca                                                                                     <  1:632798/151‑1 (MQ=255)
                                                           gCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTtcatca                                                                                     <  1:401048/151‑1 (MQ=255)
                                                                aGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGTCTATCGGGTGGGTTCGGTTGAAACCAGCTCCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCa                                                                                <  2:88350/151‑1 (MQ=255)
                                                                  gTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCAcc                                                                              >  2:596152/1‑151 (MQ=255)
                                                                      ccGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTAt                                                                          <  1:680395/151‑1 (MQ=255)
                                                                        gtgtGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATg                                                                         >  1:46674/1‑150 (MQ=255)
                                                                            gATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCg                                                                     <  2:582890/150‑1 (MQ=255)
                                                                             aTTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGAc                                                                   >  2:284484/1‑151 (MQ=255)
                                                                               tctcGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACt                                                                  <  1:20036/150‑1 (MQ=255)
                                                                                  cGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTg                                                                 <  1:279894/148‑1 (MQ=255)
                                                                                    aTAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGAc                                                            <  1:391095/151‑1 (MQ=255)
                                                                                    aTAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTACTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGAc                                                            >  2:483678/1‑151 (MQ=255)
                                                                                             aaGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGt                                                   <  2:250154/151‑1 (MQ=255)
                                                                                               gCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTc                                                 >  2:154581/1‑151 (MQ=255)
                                                                                                    gCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTc                                            >  1:399042/1‑151 (MQ=255)
                                                                                                          ctcGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGaa                                       >  1:403666/1‑150 (MQ=255)
                                                                                                                       ccGGTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTg                         >  2:444115/1‑151 (MQ=255)
                                                                                                                         ggTGCTGTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCg                       <  1:521132/151‑1 (MQ=255)
                                                                                                                               gTTCCGTGGCTATCGGGTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGa                  <  2:284858/150‑1 (MQ=255)
                                                                                                                                            cGGGTGGGTTCGGTTGAAACCAGCACCTTCGGTACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTcagc      >  1:401557/1‑149 (MQ=255)
                                                                                                                                              ggTGGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAggg  <  2:24278/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGG  >  NC_000913/1013662‑1013954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A