Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,516	A→G	V377A (GTC→GCC)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,516	0	A	G	100.0%	136.9 / NA	39	V377A (GTC→GCC)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/24); total (15/24)

ACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGC  >  NC_000913/1307368‑1307661

aCTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGcc                                                                                                                                                 <  2:51332/151‑1 (MQ=255)

  tACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGcc                                                                                                                                                 >  1:197306/1‑149 (MQ=255)

   aCGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAg                                                                                                                                               <  2:543108/150‑1 (MQ=255)

     gCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCAt                                                                                                                                            <  1:382108/151‑1 (MQ=255)

      cATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATc                                                                                                                                           >  1:265400/1‑151 (MQ=255)

        tatCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCg                                                                                                                                          >  2:571867/1‑150 (MQ=255)

        tatCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCg                                                                                                                                          >  2:147596/1‑150 (MQ=255)

                        gCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCgg                                                                                                                          <  1:225237/150‑1 (MQ=255)

                         ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGaa                                                                                                                        <  2:23146/151‑1 (MQ=255)

                         ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGaa                                                                                                                        <  1:558927/151‑1 (MQ=255)

                              cAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGat                                                                                                                    >  2:61410/1‑150 (MQ=255)

                                   ttAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACt                                                                                                              >  2:87133/1‑151 (MQ=255)

                                    tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATAc                                                                                                               <  2:123522/149‑1 (MQ=255)

                                    tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACt                                                                                                              <  1:546969/150‑1 (MQ=255)

                                         cGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATc                                                                                                        <  1:147601/151‑1 (MQ=255)

                                                  ccAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCAcccc                                                                                                  <  2:414760/148‑1 (MQ=255)

                                                         gCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGcgcc                                                                                        <  1:374038/151‑1 (MQ=255)

                                                          ctctTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGcgccg                                                                                       >  2:125767/1‑151 (MQ=255)

                                                           tctTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGcgccgc                                                                                      >  1:379992/1‑151 (MQ=255)

                                                              tGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGccgccg                                                                                    <  2:241643/150‑1 (MQ=255)

                                                               ggTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGt                                                                                   <  1:78506/150‑1 (MQ=255)

                                                               ggTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGt                                                                                   <  2:237964/150‑1 (MQ=255)

                                                                           cccGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGa                                                                      <  2:202595/151‑1 (MQ=255)

                                                                                            aaaTTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTgg                                                     <  2:397363/151‑1 (MQ=255)

                                                                                                tttAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGcac                                                  >  1:664184/1‑150 (MQ=255)

                                                                                                   aaCCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAg                                              <  2:390422/151‑1 (MQ=255)

                                                                                                           cagccagcagTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGt                                      >  2:513199/1‑151 (MQ=255)

                                                                                                            agccagcagTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCg                                       <  2:659243/149‑1 (MQ=255)

                                                                                                                   agTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGa                              <  1:571884/151‑1 (MQ=255)

                                                                                                                    gTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAt                             <  1:32210/151‑1 (MQ=255)

                                                                                                                          tAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAat                        >  2:334940/1‑150 (MQ=255)

                                                                                                                                 tGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgc                >  2:433507/1‑151 (MQ=255)

                                                                                                                                  gcgcGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgcg               <  1:654346/151‑1 (MQ=255)

                                                                                                                                     cgACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCg            <  1:99062/151‑1 (MQ=255)

                                                                                                                                          ggCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATaa       <  1:181155/151‑1 (MQ=255)

                                                                                                                                           gCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATa        >  2:159541/1‑149 (MQ=255)

                                                                                                                                             ccAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGcc    <  2:363063/151‑1 (MQ=255)

                                                                                                                                               aGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc  >  1:26479/1‑151 (MQ=255)

                                                                                                                                                 ccGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc  >  1:128759/1‑149 (MQ=255)

ACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGC  >  NC_000913/1307368‑1307661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A