Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,099	C→T	G183R (GGG→AGG)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,099	0	C	T	100.0%	128.4 / NA	36	G183R (GGG→AGG)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/18); total (18/18)

TATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATT  >  NC_000913/1307952‑1308244

tATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCtgg                                                                                                                                                 <  2:207133/150‑1 (MQ=255)

      tcatgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          >  1:95921/1‑151 (MQ=255)

       catgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          <  2:601611/150‑1 (MQ=255)

       catgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          >  1:601629/1‑150 (MQ=255)

        atgatcatCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTc                                                                                                                                          <  2:156711/149‑1 (MQ=255)

            tcatcTTGCGATGTTGGCGCAGGTCCATACAGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCa                                                                                                                                     >  1:478674/1‑150 (MQ=255)

                    cGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGc                                                                                                                            >  1:533300/1‑151 (MQ=255)

                           ggCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCaatgaat                                                                                                                     <  1:615030/151‑1 (MQ=255)

                            gcgcAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCaatgaatg                                                                                                                    <  1:159546/151‑1 (MQ=255)

                                        aCGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCa                                                                                                        <  1:100068/151‑1 (MQ=255)

                                         cGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCgcgc                                                                                                         >  1:661749/1‑149 (MQ=255)

                                               aaaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAgccgcc                                                                                                  <  2:177044/150‑1 (MQ=255)

                                               aaaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAgccgcc                                                                                                  >  1:573202/1‑150 (MQ=255)

                                               aaaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCa                                                                                                 <  2:360230/151‑1 (MQ=255)

                                                aaaaCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCAt                                                                                                <  1:361040/151‑1 (MQ=255)

                                                                        ttAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCGCGGGCCACGGGCGGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAACCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTcc                                                                          <  2:309638/149‑1 (MQ=255)

                                                                           aGGCTTCGACCACTTCAATCCCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACCCTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCAt                                                                     <  1:145557/151‑1 (MQ=255)

                                                                           aGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCAt                                                                     >  1:346388/1‑151 (MQ=255)

                                                                                tCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATgccgc                                                                >  1:310561/1‑151 (MQ=255)

                                                                                 cGACCCCTTCAATACCGGCATTACGCATTAGCGCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGGGCATCAACCGGCAATGACTGCCGCGCCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATgccgcc                                                               <  2:670687/151‑1 (MQ=255)

                                                                                  gACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGccgccg                                                              >  2:523678/1‑151 (MQ=255)

                                                                                                cGGCATTACGCATTAGCCCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGAtata                                                <  2:248101/151‑1 (MQ=255)

                                                                                                    aTTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAac                                            >  1:62023/1‑151 (MQ=255)

                                                                                                    aTTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAac                                            >  2:164564/1‑151 (MQ=255)

                                                                                                     ttACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAac                                            >  2:157749/1‑150 (MQ=255)

                                                                                                         gCATTGGCTCGAGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATaaaa                                             <  2:128755/145‑1 (MQ=255)

                                                                                                         gCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAt                                       >  1:605085/1‑151 (MQ=255)

                                                                                                          cATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAtc                                      >  2:483401/1‑151 (MQ=255)

                                                                                                           aTTAGCTCGGTCCACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCAtct                                     >  1:332539/1‑151 (MQ=255)

                                                                                                                       cACGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATg                           <  2:62534/149‑1 (MQ=255)

                                                                                                                        aCGGGCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgc                         >  2:294226/1‑150 (MQ=255)

                                                                                                                          ggccTGCGGAAAAAAGCCACACCCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGgcgc                       <  2:29523/147‑1 (MQ=255)

                                                                                                                            gCTGCGGAAAAAAGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGcgcgcg                    >  2:502045/1‑151 (MQ=255)

                                                                                                                                    aaaaaGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGAt            <  1:147708/151‑1 (MQ=255)

                                                                                                                                      aaaGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGt          <  1:223722/151‑1 (MQ=255)

                                                                                                                                      aaaGCCACACTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGt          >  1:99742/1‑151 (MQ=255)

                                                                                                                                               cTCCTGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGAtt  <  1:241500/150‑1 (MQ=255)

TATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCCGCCATTAATGATTCAGCCACCTGGTCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATT  >  NC_000913/1307952‑1308244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A