Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,324,641	A→G	T182A (ACA→GCA)	yciO →	putative RNA binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,324,641	0	A	G	100.0%	138.4 / NA	40	T182A (ACA→GCA)	yciO	putative RNA binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/20); total (20/20)

TTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCA  >  NC_000913/1324495‑1324784

ttGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAAc                                                                                                                                             >  1:205911/1‑151 (MQ=255)

    aGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGtt                                                                                                                                         <  2:664454/151‑1 (MQ=255)

         tGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTg                                                                                                                                     <  1:68474/150‑1 (MQ=255)

          gggCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGAt                                                                                                                                   <  1:35846/151‑1 (MQ=255)

               aaCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTAc                                                                                                                              >  1:394718/1‑151 (MQ=255)

                aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTAcc                                                                                                                             <  1:304177/151‑1 (MQ=255)

                aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCCCGGGCAGAAACCGGCAACGGCTATTGATCTTaac                                                                                                                             >  2:361253/1‑149 (MQ=255)

                         ttCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATa                                                                                                                      <  2:71990/149‑1 (MQ=255)

                            cACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCg                                                                                                                 >  1:359771/1‑151 (MQ=255)

                             aCTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCgg                                                                                                                >  1:34152/1‑151 (MQ=255)

                                  gCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCgtg                                                                                                           >  2:139551/1‑151 (MQ=255)

                                     aaTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCgtgg                                                                                                          <  2:514918/149‑1 (MQ=255)

                                     aaTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGGTATTGATCTTACCGATGATACGCCGGTCgtggt                                                                                                         >  1:27045/1‑150 (MQ=255)

                                      aTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGc                                                                                                       <  2:327870/151‑1 (MQ=255)

                                        gctgcCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCg                                                                                                      <  2:510311/150‑1 (MQ=255)

                                              aGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAg                                                                                                 >  1:620914/1‑149 (MQ=255)

                                                       aTTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAt                                                                                      >  1:285534/1‑151 (MQ=255)

                                                         ttACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtg                                                                                     <  1:129482/150‑1 (MQ=255)

                                                             cGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAg                                                                                <  1:291895/151‑1 (MQ=255)

                                                                aTCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCt                                                                             >  2:298624/1‑151 (MQ=255)

                                                                   ggACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCttt                                                                           >  2:643857/1‑150 (MQ=255)

                                                                    gACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCt                                                                         >  2:598092/1‑151 (MQ=255)

                                                                    gACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCt                                                                         <  2:659884/151‑1 (MQ=255)

                                                                        cGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTtata                                                                     <  1:300763/151‑1 (MQ=255)

                                                                               aaTTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAAtt                                                              <  1:460069/151‑1 (MQ=255)

                                                                                 ttAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGc                                                            >  2:396627/1‑151 (MQ=255)

                                                                                          gTCTGGAAAAACAGGTAGATTTGATTATTCGCGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTTCCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACg                                                    <  2:504696/150‑1 (MQ=255)

                                                                                            cTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGAt                                                 >  2:446992/1‑151 (MQ=255)

                                                                                                aaaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGa                                              >  2:397955/1‑150 (MQ=255)

                                                                                                  aaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGa                                              >  2:343540/1‑148 (MQ=255)

                                                                                                     cAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGGTGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACt                                         >  2:571/1‑150 (MQ=255)

                                                                                                         tAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGc                                    >  1:606599/1‑151 (MQ=255)

                                                                                                                 attattCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGt                            <  1:277376/151‑1 (MQ=255)

                                                                                                                  ttattCACGGCGGCTATCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGtt                           >  2:268279/1‑151 (MQ=255)

                                                                                                                                 aTCTCGGGCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTg              <  2:562721/149‑1 (MQ=255)

                                                                                                                                      gggCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGa       <  1:157414/151‑1 (MQ=255)

                                                                                                                                      gggCAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGa       <  1:194777/151‑1 (MQ=255)

                                                                                                                                         cAGAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAg    <  2:2247/151‑1 (MQ=255)

                                                                                                                                           gAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGc   >  2:123225/1‑150 (MQ=255)

                                                                                                                                           gAAACCGGCAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCa  <  2:32886/151‑1 (MQ=255)

TTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCA  >  NC_000913/1324495‑1324784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A