Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F50 I2 R1
|
209 |
44.2 |
1360995 |
98.7% |
1343302 |
150.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,383,697 |
C→T |
A89T (GCC→ACC) |
ycjW ← |
LacI family putative transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,383,697 | 0 | C | T | 93.6%
| 143.9
/ NA
| 48 | A89T (GCC→ACC) | ycjW | LacI family putative transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (24/20); minor base G (0/3); total (25/23) |
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGG > NC_000913/1383552‑1383843
|
aaaaaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGgtggtg > 2:283156/1‑150 (MQ=255)
aaaaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGAt > 2:598627/1‑151 (MQ=255)
aaTGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGATTTGGTGGTGATgg < 2:272820/151‑1 (MQ=255)
gACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCa < 1:283161/150‑1 (MQ=255)
gACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCa < 1:419009/150‑1 (MQ=255)
cATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAAATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTTGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCa > 2:133573/1‑150 (MQ=255)
ccGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAAc < 2:342646/151‑1 (MQ=255)
gCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACtt < 2:557301/151‑1 (MQ=255)
gCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCaga < 2:144404/151‑1 (MQ=255)
cGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCag > 1:584411/1‑149 (MQ=255)
gAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGaaaa < 2:178361/149‑1 (MQ=255)
gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACgg < 1:584995/151‑1 (MQ=255)
gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACgg > 2:212454/1‑151 (MQ=255)
taatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTAcc > 2:524431/1‑151 (MQ=255)
aatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTAccc > 1:233023/1‑151 (MQ=255)
ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTCCAGCTTTGGTGGTGATGGCATGCAAACCTTCAGAGAAAAACGGATTTCccgccg < 2:501588/151‑1 (MQ=255)
tttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCGGCCGt > 2:334948/1‑150 (MQ=255)
tttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGt > 1:123841/1‑150 (MQ=255)
tCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGtttt > 1:538442/1‑151 (MQ=255)
tCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGtttt > 2:426933/1‑151 (MQ=255)
cTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGa > 1:386504/1‑150 (MQ=255)
cTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGa > 1:37474/1‑150 (MQ=255)
gCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGCCGTTTTTAATATCACGCCGAAACCTTCTTCTTCAGTTTTGTTGGTGATGGAAGGCAAACCTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAAc < 1:157777/150‑1 (MQ=255)
atttttGTTTTCCCATTTTTGCAGATCTTCTGCCGGTTTGTGCGACTTTTGAAATATCACGTCGAACCCTTCTTTTTCAGTTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGTTTACCCGCCGTAGTTTTGGTCGAACggg < 1:311503/147‑1 (MQ=255)
aaTTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGt > 1:130934/1‑150 (MQ=255)
ttttGTTTTCGCATTTTTGCAGTTCTTCTGCCGGATTGTGCGACGTTTGTAATATCCCGTCGAACCCTTCTTTTTCAGCTTTGGTGGTAATGGCATGCAAACCTCCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGt < 2:439567/148‑1 (MQ=255)
ttttGCTTCCGCTTTTTTGCAGATCTTCTGCGGGATTGTGCGACGTTTGTAATATCACGTCAAACCCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTGCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGt < 1:623388/148‑1 (MQ=255)
tCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc > 1:25382/1‑150 (MQ=255)
tttGCAGATCTTCTGCCGGATTGTGCGGCGTTTGTAATATCACGTCAAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATgg < 1:269229/150‑1 (MQ=255)
aGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCa > 2:299414/1‑150 (MQ=255)
aTCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaa > 1:257025/1‑150 (MQ=255)
tcttctGCCGGATTGTGCGACGTTTGTAATATCCCGTCAAACCTTTCTTCTTCATTTTTGGTGGTGATGGCATGCAAACCTTCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaaa < 1:228823/150‑1 (MQ=255)
cttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAcc > 2:94571/1‑151 (MQ=255)
tctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCg > 2:346354/1‑151 (MQ=255)
tGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGa > 1:276285/1‑150 (MQ=255)
ttGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTc > 2:545270/1‑150 (MQ=255)
tgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcg > 2:70082/1‑151 (MQ=255)
gagttttGTAATATCACGCCGAACCCTTTTTCTCCAGC‑‑TGGTGGTGATGGCATGCAAACCTTCAAAAAAAACCGTTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGcgcgcg < 1:101857/146‑1 (MQ=255)
cGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCgg > 2:590749/1‑151 (MQ=255)
atatCCCGTCAAACCCTTCTTTTTAAGCTTGGGTGGTGATGGAATGAAAACCTTAAGAAAAAACCGTTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCt < 2:562190/150‑1 (MQ=255)
cccGTCGAACCCTTCTTCTTCAGTTTGGGGGGTGATGGCATGCAAAACTTAAAAAAAAACCGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGttt < 2:177210/149‑1 (MQ=255)
cACGTCAAACCCTTCTTCTTCAGTTTTGGTGGTGAGGGCATGCAAAACTTCAGAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTg < 1:439695/148‑1 (MQ=255)
acggcaaacccTTCTTCTTCAGCTTTGGTGGTGATGGCATGAAAACCTTCAAAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGttt < 1:317530/141‑1 (MQ=255)
cGTCGAAACCTTCTTCTTCAGCTTTGGGGGTGATGGCATGAAAACCTCCAGAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTg < 2:141024/150‑1 (MQ=255)
gccaaaaCCTTTTTCTCCAGCTTGGGGGGTGATGGCATGCAAAACTTCAGAAAAAACGGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTg < 1:245271/145‑1 (MQ=255)
aaaCCTTCTTCTTCAGCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGAt > 1:357280/1‑151 (MQ=255)
tcatttttGGTGGTGTGGTCATGCAAAACTTAAGAAAAAAAGGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAAtt < 2:649132/143‑1 (MQ=255)
gCTTTGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCa > 1:492541/1‑150 (MQ=255)
ttGGTGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATgg > 2:301380/1‑151 (MQ=255)
|
AAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGG > NC_000913/1383552‑1383843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
