Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,603,562	T→C	F182L (TTT→CTT)	lsrC →	autoinducer 2 import system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,603,562	0	T	C	100.0%	117.4 / NA	32	F182L (TTT→CTT)	lsrC	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/15); total (17/15)

GTGGACTGGCGGCAAATGGATTGAAGGGTTACCCGCCGAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATT  >  NC_000913/1603420‑1603705

gtgGACTGGCGGCAAATGGATTGAAGGGTTACCCGCCGAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGAc                                                                                                                                           >  1:645325/1‑149 (MQ=255)

         cggcAAATGGATTGAAGGGTTACCCGCCGAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTAt                                                                                                                                >  1:593805/1‑151 (MQ=255)

           gcAAATGGATTGAAGGGTTACCCGCCGAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGc                                                                                                                              >  2:515063/1‑151 (MQ=255)

                                 cgccgAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGgc                                                                                                        >  2:412141/1‑151 (MQ=255)

                                  gccgAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGgcg                                                                                                       >  2:139850/1‑151 (MQ=255)

                                    cgAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCt                                                                                                     <  2:77273/151‑1 (MQ=255)

                                     gAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTc                                                                                                    <  2:553855/151‑1 (MQ=255)

                                     gAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTc                                                                                                    >  2:446262/1‑151 (MQ=255)

                                          gAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCa                                                                                                >  1:191443/1‑150 (MQ=255)

                                           aaaCAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAAc                                                                                              >  2:193858/1‑151 (MQ=255)

                                                gCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGCCGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGCTAATTTACAGGGCGCTCGTCAACTGGGc                                                                                         >  2:668387/1‑151 (MQ=255)

                                                     ccGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGtt                                                                                      <  2:589750/149‑1 (MQ=255)

                                                      cGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGt                                                                                   >  2:489779/1‑151 (MQ=255)

                                                       gCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTa                                                                                  <  1:560227/151‑1 (MQ=255)

                                                            gctgctgctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGa                                                                              >  1:617402/1‑150 (MQ=255)

                                                             ctgctgctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGa                                                                              >  2:657723/1‑149 (MQ=255)

                                                              tgctgctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGc                                                                           >  2:384544/1‑151 (MQ=255)

                                                                 tgctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCAt                                                                        >  1:341218/1‑151 (MQ=255)

                                                                  gctTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCAtt                                                                       <  2:316603/151‑1 (MQ=255)

                                                                     tGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTc                                                                      <  1:499914/149‑1 (MQ=255)

                                                                      ggCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCa                                                                   <  1:5701/151‑1 (MQ=255)

                                                                           ttCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATtgt                                                               <  2:521328/150‑1 (MQ=255)

                                                                             cAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTgg                                                             <  1:405867/150‑1 (MQ=255)

                                                                                  aTTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCAtttt                                                       <  2:315080/151‑1 (MQ=255)

                                                                                            tgACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACg                                              <  1:207822/150‑1 (MQ=255)

                                                                                              aCGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCt                                           <  1:498469/151‑1 (MQ=255)

                                                                                               cGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTg                                          <  1:653845/151‑1 (MQ=255)

                                                                                                      tCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATggc                                    <  1:640943/150‑1 (MQ=255)

                                                                                                                          ggCTGCTGGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATtgtg                 <  2:120049/149‑1 (MQ=255)

                                                                                                                                tgGCAAAGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCtt          >  1:397084/1‑150 (MQ=255)

                                                                                                                                      aGACGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTTCTTCGCCGAt   >  1:481384/1‑151 (MQ=255)

                                                                                                                                         cGGCGCTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTTTGTTTTCTTTGCAGGtt  >  2:207950/1‑149 (MQ=255)

GTGGACTGGCGGCAAATGGATTGAAGGGTTACCCGCCGAACTGAAACAGCTCTCCGCCCCGCTGCTGCTTGGCGTTTCAGCAATTGGTTGGTTGACGATAATTCTGGTGGCATTTATGGCCTGGCTGCTGGCAAAGACGGCGTTTGGACGCAGTTTTTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATT  >  NC_000913/1603420‑1603705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A