Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,784	A→G	S356S (AGT→AGC)	uidC ←	putative outer membrane porin for beta‑glucuronides porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,784	0	A	G	100.0%	121.3 / NA	33	S356S (AGT→AGC)	uidC	putative outer membrane porin for beta‑glucuronides porin protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

TCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTACTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCC  >  NC_000913/1691636‑1691931

tCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCt                                                                                                                                                   >  1:558718/1‑151 (MQ=255)

       aTCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTc                                                                                                                                             >  1:71182/1‑150 (MQ=255)

         cGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCAt                                                                                                                                           <  1:572019/150‑1 (MQ=255)

         cGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATa                                                                                                                                          <  1:612662/151‑1 (MQ=255)

                      gCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCGCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTcaca                                                                                                                             <  1:227149/151‑1 (MQ=255)

                        cATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAAt                                                                                                                           <  2:564246/151‑1 (MQ=255)

                             cgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTg                                                                                                                      >  2:122339/1‑151 (MQ=255)

                             cgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTg                                                                                                                      <  2:441517/151‑1 (MQ=255)

                              gcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGc                                                                                                                     >  2:308516/1‑151 (MQ=255)

                                    cccGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGt                                                                                                               >  2:530028/1‑151 (MQ=255)

                                    cccGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGt                                                                                                               <  2:143052/151‑1 (MQ=255)

                                            ttATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTa                                                                                                        >  1:359727/1‑150 (MQ=255)

                                                cGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTg                                                                                                   >  1:375190/1‑151 (MQ=255)

                                                  gcagcaAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTgcg                                                                                                 <  1:169975/151‑1 (MQ=255)

                                                   cagcaAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCGCCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCTCAATCGGTGCAAATGTCAGCGTAAATTgcg                                                                                                 <  2:66941/150‑1 (MQ=255)

                                                       aagaaagaAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGtt                                                                                            >  2:162980/1‑151 (MQ=255)

                                                       aagaaagaAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGtt                                                                                            <  1:159968/151‑1 (MQ=255)

                                                               aaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGAt                                                                                     >  1:525230/1‑150 (MQ=255)

                                                                aTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAg                                                                                   <  2:468009/151‑1 (MQ=255)

                                                                           gTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGccc                                                                          <  2:576039/149‑1 (MQ=255)

                                                                             aaCGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAAtt                                                                      <  2:515052/151‑1 (MQ=255)

                                                                                    cTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAgg                                                                >  1:161469/1‑150 (MQ=255)

                                                                                       cGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTg                                                            >  1:575921/1‑151 (MQ=255)

                                                                                       cGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTg                                                            <  1:123802/151‑1 (MQ=255)

                                                                                            aCCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTc                                                       <  2:98241/151‑1 (MQ=255)

                                                                                                      tCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGtt                                             >  2:417860/1‑151 (MQ=255)

                                                                                                        aTACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTAc                                           <  2:429542/151‑1 (MQ=255)

                                                                                                             accTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATc                                       >  1:355344/1‑150 (MQ=255)

                                                                                                                  aGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACt                                 >  2:559794/1‑151 (MQ=255)

                                                                                                                                tCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAggg                     <  2:557839/149‑1 (MQ=255)

                                                                                                                                            tctcGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCgg       >  2:199173/1‑151 (MQ=255)

                                                                                                                                               cGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCg        <  1:530833/147‑1 (MQ=255)

                                                                                                                                                 tttGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATcc  >  1:34221/1‑151 (MQ=255)

TCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTACTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCC  >  NC_000913/1691636‑1691931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A