Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,717,165	T→C	T20A (ACG→GCG)	tyrS ←	tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,717,165	0	T	C	100.0%	95.6 / NA	29	T20A (ACG→GCG)	tyrS	tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/17); total (12/17)

TGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAT  >  NC_000913/1717024‑1717311

tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTgg                                                                                                                                             <  1:660265/149‑1 (MQ=255)

tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTgg                                                                                                                                             <  1:448888/149‑1 (MQ=255)

     cgcGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGTTGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCACGTCCGCCACCTGGGCTACCa                                                                                                                                      <  2:158157/149‑1 (MQ=255)

            gctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGcccc                                                                                                                                 <  2:177714/149‑1 (MQ=255)

              tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGc                                                                                                                              >  1:117406/1‑150 (MQ=255)

              tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGACGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGct                                                                                                                             >  2:530877/1‑151 (MQ=255)

                           ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGttt                                                                                                                >  1:247636/1‑151 (MQ=255)

                                aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                                            >  1:342142/1‑150 (MQ=255)

                                      ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTa                                                                                                      <  2:606860/150‑1 (MQ=255)

                                      ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAc                                                                                                     <  1:437480/151‑1 (MQ=255)

                                            aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                                               <  2:60562/151‑1 (MQ=255)

                                                        tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                                                   >  1:20397/1‑151 (MQ=255)

                                                                  cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                                                          <  1:350164/150‑1 (MQ=255)

                                                                      ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                                                     >  1:122103/1‑151 (MQ=255)

                                                                         aGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                                                  <  1:666325/151‑1 (MQ=255)

                                                                                        aTAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTa                                                   <  1:445989/151‑1 (MQ=255)

                                                                                               gcgATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACt                                            >  2:166050/1‑151 (MQ=255)

                                                                                                  aTCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt                                          <  2:176033/150‑1 (MQ=255)

                                                                                                       gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCGCCTTTGCCGGTACACGACTTTTCGCCa                                    <  2:187866/151‑1 (MQ=255)

                                                                                                       gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGcc                                     >  1:673418/1‑150 (MQ=255)

                                                                                                       gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCa                                    <  1:495812/151‑1 (MQ=255)

                                                                                                         cTTGCGCCAGTCGCTCTGCTAACGCTCCCTCGTCCGCCACCTGGCCTACCAGCCCCCGCTCTTGCAATCGTTTATCCAAGTCACTGCTTGCTATCAATATCTCCATGTATATAACTATTGTACCTATGCCGGTCCACGACTTTTCCCCa                                    >  2:366532/1‑149 (MQ=255)

                                                                                                           tGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGa                                  <  1:591993/149‑1 (MQ=255)

                                                                                                             cgcCAGTCGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg                              >  1:184579/1‑151 (MQ=255)

                                                                                                                    cGCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAagag                        <  2:435579/150‑1 (MQ=255)

                                                                                                                     gCTCTGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGAc                      <  1:171153/151‑1 (MQ=255)

                                                                                                                         tGCTAACGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCACAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACAt                    >  2:363781/1‑149 (MQ=255)

                                                                                                                              aCGCTTCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaa             <  2:273771/151‑1 (MQ=255)

                                                                                                                                  ttCCTCGTCCGCCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTg         >  2:607467/1‑151 (MQ=255)

                                                                                                                                         tccgccACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAt  >  2:471026/1‑151 (MQ=255)

TGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAT  >  NC_000913/1717024‑1717311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A