Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,955,053	T→C	H652R (CAC→CGC)	torZ ←	trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,955,053	0	T	C	100.0%	176.7 / NA	48	H652R (CAC→CGC)	torZ	trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/23); total (25/23)

TCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGGC  >  NC_000913/1954909‑1955197

tCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCg                                                                                                                                            >  2:160014/1‑151 (MQ=255)

   cGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGc                                                                                                                                         >  2:174862/1‑151 (MQ=255)

      tCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAAt                                                                                                                                      >  1:336458/1‑151 (MQ=255)

         gCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTTCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCt                                                                                                                                   <  1:367467/151‑1 (MQ=255)

         gCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCt                                                                                                                                   <  1:613691/151‑1 (MQ=255)

          cGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCtt                                                                                                                                  <  1:409280/151‑1 (MQ=255)

            aTCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTtat                                                                                                                                >  2:567816/1‑151 (MQ=255)

              cGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTtata                                                                                                                               <  2:434978/150‑1 (MQ=255)

                   atTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCaa                                                                                                                          >  1:475605/1‑150 (MQ=255)

                     tttttACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAAtt                                                                                                                       >  2:504011/1‑151 (MQ=255)

                       tttACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAAttt                                                                                                                      <  2:636053/150‑1 (MQ=255)

                           cGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTcc                                                                                                                  >  1:624114/1‑150 (MQ=255)

                             cAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAAc                                                                                                               <  1:94659/151‑1 (MQ=255)

                              aGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACg                                                                                                              >  1:403926/1‑151 (MQ=255)

                               gTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGt                                                                                                             >  2:468753/1‑151 (MQ=255)

                                ttCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGtt                                                                                                            >  1:409760/1‑151 (MQ=255)

                                   cGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGttttt                                                                                                         >  2:514940/1‑151 (MQ=255)

                                   cGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGttttt                                                                                                         >  1:204511/1‑151 (MQ=255)

                                      aTAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGaa                                                                                                      <  2:206697/151‑1 (MQ=255)

                                         gTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAAt                                                                                                     >  1:6990/1‑149 (MQ=255)

                                            aaGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGAtt                                                                                                <  2:381239/151‑1 (MQ=255)

                                              gCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTc                                                                                              <  2:449301/151‑1 (MQ=255)

                                                     gtgtAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAAttt                                                                                         <  1:155664/149‑1 (MQ=255)

                                                     gtgtAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGACAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAAtttt                                                                                        >  2:641613/1‑150 (MQ=255)

                                                        tAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCg                                                                                    >  2:25445/1‑151 (MQ=255)

                                                         aaaCGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCg                                                                                    <  2:483085/150‑1 (MQ=255)

                                                         aaaCGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGc                                                                                   >  1:493549/1‑151 (MQ=255)

                                                              gtgtGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGc                                                                              <  2:657754/151‑1 (MQ=255)

                                                                  gCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTAc                                                                          <  1:41971/151‑1 (MQ=255)

                                                                  gCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTAc                                                                          >  2:144807/1‑151 (MQ=255)

                                                                                              gCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGgcg                                                <  1:233579/149‑1 (MQ=255)

                                                                                              gCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGcgcg                                              <  1:206144/151‑1 (MQ=255)

                                                                                              gCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGcgcg                                              <  2:50370/151‑1 (MQ=255)

                                                                                                     gtcgGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAAt                                         <  2:366567/149‑1 (MQ=255)

                                                                                                     gtcgGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCa                                       <  2:475593/151‑1 (MQ=255)

                                                                                                       cggcggTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCAcc                                     >  1:394084/1‑151 (MQ=255)

                                                                                                                 tttCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATaacgaacg                           >  1:410690/1‑151 (MQ=255)

                                                                                                                  ttCCATTCATCAGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGt                          >  1:375250/1‑151 (MQ=255)

                                                                                                                          atcaGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTgttcg                  <  1:46984/151‑1 (MQ=255)

                                                                                                                            caGGAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTgttcgtt                <  2:516963/151‑1 (MQ=255)

                                                                                                                               gAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGttctt             <  1:625500/151‑1 (MQ=255)

                                                                                                                               gAGCCAGCCAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGttctt             >  2:277770/1‑151 (MQ=255)

                                                                                                                                agccagccAGGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGttcttc            >  1:343834/1‑151 (MQ=255)

                                                                                                                                   cagccagGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCg         >  1:455676/1‑151 (MQ=255)

                                                                                                                                    agccagGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGc        >  1:457256/1‑151 (MQ=255)

                                                                                                                                    agccagGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGc        >  1:621212/1‑151 (MQ=255)

                                                                                                                                       cagGTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCtgt     <  2:375240/151‑1 (MQ=255)

                                                                                                                                          gTTGGGCGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGgc  <  1:260018/151‑1 (MQ=255)

TCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGGC  >  NC_000913/1954909‑1955197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A