Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,955,440	T→C	Q523R (CAG→CGG)	torZ ←	trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,955,440	0	T	C	100.0%	127.0 / NA	37	Q523R (CAG→CGG)	torZ	trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/15); total (22/15)

AAATTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATG  >  NC_000913/1955292‑1955575

aaaTTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCgg                                                                                                                                       >  2:124614/1‑151 (MQ=255)

          cACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAAt                                                                                                                             <  1:242018/151‑1 (MQ=255)

               cATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCg                                                                                                                        >  1:354233/1‑151 (MQ=255)

                  ttCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTc                                                                                                                     >  1:462042/1‑151 (MQ=255)

                  ttCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTc                                                                                                                     >  2:223898/1‑151 (MQ=255)

                   tCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTc                                                                                                                     >  1:205223/1‑150 (MQ=255)

                    cATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCa                                                                                                                    <  2:593060/150‑1 (MQ=255)

                       cTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGt                                                                                                                >  1:2818/1‑151 (MQ=255)

                         ttACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTc                                                                                                               >  2:235251/1‑150 (MQ=255)

                                      aGATCTCTTTGCCGCCAGGTTGGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc                                                                                                 <  2:292919/151‑1 (MQ=255)

                                              tttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTGAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                                                                         >  1:337803/1‑151 (MQ=255)

                                              tttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAt                                                                                          <  1:377258/150‑1 (MQ=255)

                                                 ccGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtgg                                                                                      >  2:318618/1‑151 (MQ=255)

                                                   gCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggtg                                                                                    <  1:468579/151‑1 (MQ=255)

                                                            gAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                                                           <  2:66222/151‑1 (MQ=255)

                                                            gAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                                                           >  2:382825/1‑151 (MQ=255)

                                                                    ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                                                    <  2:265871/150‑1 (MQ=255)

                                                                    ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                                                    <  2:2818/150‑1 (MQ=255)

                                                                     cGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCt                                                                  <  2:573444/151‑1 (MQ=255)

                                                                                         cAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGt                                              >  2:456123/1‑151 (MQ=255)

                                                                                                  tACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGc                                      <  2:317677/150‑1 (MQ=255)

                                                                                                    cgcgCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCAtt                                   >  1:547429/1‑151 (MQ=255)

                                                                                                     gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTc                                  >  1:71572/1‑151 (MQ=255)

                                                                                                      cgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCa                                 <  2:133682/151‑1 (MQ=255)

                                                                                                         ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGa                               >  2:125926/1‑150 (MQ=255)

                                                                                                         ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGCGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGa                               >  2:275543/1‑150 (MQ=255)

                                                                                                           cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGaaa                             <  2:1475/150‑1 (MQ=255)

                                                                                                           cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAac                            >  1:202808/1‑151 (MQ=255)

                                                                                                            aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacg                           >  2:392279/1‑151 (MQ=255)

                                                                                                             aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacga                          <  2:337796/151‑1 (MQ=255)

                                                                                                             aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacga                          <  2:314960/151‑1 (MQ=255)

                                                                                                              aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacgac                         >  1:356117/1‑151 (MQ=255)

                                                                                                                  cGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGat                      <  2:340151/150‑1 (MQ=255)

                                                                                                                             ccTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGttt          >  1:355367/1‑151 (MQ=255)

                                                                                                                                gCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGATGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTg       >  1:266429/1‑151 (MQ=255)

                                                                                                                                     aTCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATg  >  1:238969/1‑151 (MQ=255)

                                                                                                                                     aTCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATg  >  2:166941/1‑151 (MQ=255)

AAATTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATG  >  NC_000913/1955292‑1955575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A