Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,955,440 T→C Q523R (CAG→CGG)  torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,955,4400TC99.4% 616.6 / NA 162Q523R (CAG→CGG) torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (90/71);  total (90/72)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

AAATTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTAATCAGA  >  NC_000913/1955292‑1955586
                                                                                                                                                    |                                                                                                                                                  
aaaTTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCgg                                                                                                                                                  >  4:873256/1‑151 (MQ=255)
  aTTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCAGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAACTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGtt                                                                                                                                                >  4:1380355/1‑151 (MQ=255)
    ttCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTg                                                                                                                                               <  2:271108/150‑1 (MQ=255)
     tCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGc                                                                                                                                              <  4:984645/150‑1 (MQ=255)
         ccACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTa                                                                                                                                          >  2:143894/1‑150 (MQ=255)
         ccACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTa                                                                                                                                          >  4:1838528/1‑150 (MQ=255)
           aCGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATc                                                                                                                                       <  4:1780842/151‑1 (MQ=255)
            cGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCa                                                                                                                                      <  1:414123/151‑1 (MQ=255)
               cATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCg                                                                                                                                   >  4:1295167/1‑151 (MQ=255)
               cATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCg                                                                                                                                   <  1:576669/151‑1 (MQ=255)
                   tCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTc                                                                                                                                <  4:272808/150‑1 (MQ=255)
                       cTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGt                                                                                                                           >  1:852082/1‑151 (MQ=255)
                         ttACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATc                                                                                                                             <  4:1067323/147‑1 (MQ=255)
                         ttACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGt                                                                                                                           <  4:483294/149‑1 (MQ=255)
                         ttACCTTCGGTATAGATCTCTTTGCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATc                                                                                                                             <  1:1445864/147‑1 (MQ=255)
                          tACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCa                                                                                                                         <  4:273378/150‑1 (MQ=255)
                            ccTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGt                                                                                                                      <  4:1226206/151‑1 (MQ=255)
                             cTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                                                                                                     >  1:75975/1‑151 (MQ=255)
                               tCGGTATATATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                                                                                                     <  4:1222340/149‑1 (MQ=255)
                                   tataGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgcg                                                                                                               >  1:55/1‑151 (MQ=255)
                                    ataGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgcgc                                                                                                              <  1:697187/151‑1 (MQ=255)
                                    ataGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgcgc                                                                                                              >  4:838780/1‑151 (MQ=255)
                                      aGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc                                                                                                            >  3:491984/1‑151 (MQ=255)
                                       gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc                                                                                                            >  1:504268/1‑150 (MQ=255)
                                        aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                                                                                           >  3:513987/1‑150 (MQ=255)
                                         tctcTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa                                                                                                          >  1:1825325/1‑150 (MQ=255)
                                           tctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                                                                       >  1:1788783/1‑151 (MQ=255)
                                           tctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                                                                       >  4:1357367/1‑151 (MQ=255)
                                           tctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                                                                       >  1:1304163/1‑151 (MQ=255)
                                              tttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                                                                                                       <  1:1886670/148‑1 (MQ=255)
                                              tttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                                                                                    >  1:1692918/1‑151 (MQ=255)
                                               ttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                                                                                    >  3:436025/1‑150 (MQ=255)
                                               ttCCGCCAGGTTTGAGCAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                                                                   >  1:1594153/1‑151 (MQ=255)
                                                   gCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggtg                                                                                               <  1:1229338/151‑1 (MQ=255)
                                                     cAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                                                                             >  4:345812/1‑151 (MQ=255)
                                                     cAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                                                                             >  1:1130577/1‑151 (MQ=255)
                                                       ggTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                                                                           >  1:778645/1‑151 (MQ=255)
                                                        gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAACCGATGTGGTGATCgg                                                                                          <  1:904122/151‑1 (MQ=255)
                                                        gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                                                                          >  4:556442/1‑151 (MQ=255)
                                                        gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                                                                          >  4:223456/1‑151 (MQ=255)
                                                          ttGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt                                                                                         >  4:1333451/1‑150 (MQ=255)
                                                               tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                                                                   >  4:1883924/1‑151 (MQ=255)
                                                                aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                                                                   >  3:497160/1‑150 (MQ=255)
                                                                aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                                                                   <  4:162718/150‑1 (MQ=255)
                                                                   tCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                                                               >  4:501411/1‑151 (MQ=255)
                                                                    ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATc                                                                              >  4:796058/1‑151 (MQ=255)
                                                                     cGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCt                                                                             <  3:162985/151‑1 (MQ=255)
                                                                      gCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTg                                                                            >  1:956202/1‑151 (MQ=255)
                                                                        aaGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCg                                                                          <  1:1521472/151‑1 (MQ=255)
                                                                             cGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGttt                                                                     >  1:964295/1‑151 (MQ=255)
                                                                             cGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGtt                                                                      <  1:55178/150‑1 (MQ=255)
                                                                              ggCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTg                                                                    >  4:1753472/1‑151 (MQ=255)
                                                                                cAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgc                                                                  >  4:1485120/1‑151 (MQ=255)
                                                                                cAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgc                                                                  >  1:717215/1‑151 (MQ=255)
                                                                                 aaaCACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgct                                                                 <  4:531937/151‑1 (MQ=255)
                                                                                   acacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAACATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgc                                                               <  1:1663361/151‑1 (MQ=255)
                                                                                    cacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgct                                                              <  4:1824146/151‑1 (MQ=255)
                                                                                      cGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCtgctgc                                                            <  1:1060036/151‑1 (MQ=255)
                                                                                      cGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCtgctgc                                                            >  1:702008/1‑151 (MQ=255)
                                                                                        tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCgg                                                          >  3:211286/1‑151 (MQ=255)
                                                                                         cAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGt                                                         >  1:1644091/1‑151 (MQ=255)
                                                                                          aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                                                        >  4:846998/1‑151 (MQ=255)
                                                                                          aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                                                        <  1:177131/151‑1 (MQ=255)
                                                                                           aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTcc                                                       >  4:1463497/1‑151 (MQ=255)
                                                                                               cGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTa                                                   <  3:172575/151‑1 (MQ=255)
                                                                                                  tACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGc                                                 >  1:1552601/1‑150 (MQ=255)
                                                                                                   aCGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCAt                                               <  4:72420/151‑1 (MQ=255)
                                                                                                   aCGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCAt                                               >  2:199636/1‑151 (MQ=255)
                                                                                                    cgcgCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCAtt                                              >  4:1152668/1‑151 (MQ=255)
                                                                                                      cgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTc                                             >  1:98081/1‑150 (MQ=255)
                                                                                                       gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTc                                             >  4:127410/1‑149 (MQ=255)
                                                                                                         ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGa                                          >  1:275220/1‑150 (MQ=255)
                                                                                                           cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGaaa                                        <  1:1362088/150‑1 (MQ=255)
                                                                                                           cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAac                                       >  1:1606681/1‑151 (MQ=255)
                                                                                                            aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacg                                      >  4:1657629/1‑151 (MQ=255)
                                                                                                              aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacgac                                    <  1:1462910/151‑1 (MQ=255)
                                                                                                               ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacgacg                                   >  1:508029/1‑151 (MQ=255)
                                                                                                                tGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacgacg                                   <  2:268243/150‑1 (MQ=255)
                                                                                                                  cGGAGCACCAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGatc                                <  1:1382077/151‑1 (MQ=255)
                                                                                                                   ggAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGatc                                >  4:1170722/1‑150 (MQ=255)
                                                                                                                   ggAGCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGatc                                >  4:1264025/1‑150 (MQ=255)
                                                                                                                      gCAACAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCAtct                            >  4:70221/1‑151 (MQ=255)
                                                                                                                        aaCAGCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTcc                          >  1:406128/1‑151 (MQ=255)
                                                                                                                            gCCTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGt                       <  1:297873/150‑1 (MQ=255)
                                                                                                                             ccTGCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGttt                     <  4:862991/151‑1 (MQ=255)
                                                                                                                                gCTTCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGATCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCCGAAACGACGACCATCTGCGGTTTCTg                  >  3:203007/1‑151 (MQ=255)
                                                                                                                                 cTTCAGCGGCACAATATGCCGGTTGCTGTAATAACCGGTCATCGTCAAGTCATTGCGCTCAAACGCTGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGc                 >  1:1419790/1‑151 (MQ=255)
                                                                                                                                  ttCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGACCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGcc                >  4:640044/1‑151 (MQ=255)
                                                                                                                                   tCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGcc                <  4:1184039/150‑1 (MQ=255)
                                                                                                                                   tCATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAAGTGGTGAGCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGc                 <  4:573932/149‑1 (MQ=255)
                                                                                                                                    cATCGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCAt              >  1:1614069/1‑151 (MQ=255)
                                                                                                                                       cGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCt           >  3:488193/1‑151 (MQ=255)
                                                                                                                                       cGGCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCt           >  1:543858/1‑151 (MQ=255)
                                                                                                                                         gCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCttt         <  2:492003/151‑1 (MQ=255)
                                                                                                                                         gCACAATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCttt         <  4:676014/151‑1 (MQ=255)
                                                                                                                                          cacaATATGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTa        <  1:1193117/151‑1 (MQ=255)
                                                                                                                                              atatGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTAATCa    <  4:726053/151‑1 (MQ=255)
                                                                                                                                              atatGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTAATCa    <  1:194187/151‑1 (MQ=255)
                                                                                                                                               tatGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTAATc     >  1:1786129/1‑149 (MQ=255)
                                                                                                                                                atGCCGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTAATCAGa  <  1:711927/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAATTTCAGCCACGCCATTTCATCTTTACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGATCATCTCCGGTTTCTGCCATGCTTTAATCAGA  >  NC_000913/1955292‑1955586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A