Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,994,904	T→C	E152E (GAA→GAG)	uvrY ←	response regulator in two‑component regulatory system with BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,994,904	0	T	C	100.0%	158.0 / NA	41	E152E (GAA→GAG)	uvrY	response regulator in two‑component regulatory system with BarA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/28); total (13/28)

GAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCCGCGCCT  >  NC_000913/1994756‑1995048

gAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTc                                                                                                                                                >  2:228296/1‑151 (MQ=255)

 aGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCa                                                                                                                                               <  1:242225/151‑1 (MQ=255)

             aTCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCa                                                                                                                                   <  2:179164/151‑1 (MQ=255)

                      aaTGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCtt                                                                                                                          <  1:179289/151‑1 (MQ=255)

                        tGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTc                                                                                                                        >  1:612776/1‑151 (MQ=255)

                            tagtttaCTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGt                                                                                                                     <  2:205828/150‑1 (MQ=255)

                              gtttaCTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGtttt                                                                                                                  <  1:433121/151‑1 (MQ=255)

                                        cATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCg                                                                                                        >  1:443608/1‑151 (MQ=255)

                                          tACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGAt                                                                                                      >  1:436461/1‑151 (MQ=255)

                                          tACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGAt                                                                                                      >  1:573017/1‑151 (MQ=255)

                                           aCGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATc                                                                                                     >  2:71515/1‑151 (MQ=255)

                                              aTAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTgg                                                                                                  >  2:311582/1‑151 (MQ=255)

                                                aGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCt                                                                                                >  1:479884/1‑151 (MQ=255)

                                                 gCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCtt                                                                                               <  2:578898/151‑1 (MQ=255)

                                                  cGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCtt                                                                                               >  1:139121/1‑150 (MQ=255)

                                                    gTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAAc                                                                                            >  1:580399/1‑151 (MQ=255)

                                                              aCCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATttgtt                                                                                  >  2:378485/1‑151 (MQ=255)

                                                                cGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGa                                                                                <  1:260719/151‑1 (MQ=255)

                                                                            gagaTTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGa                                                                     <  2:345175/150‑1 (MQ=255)

                                                                               aTTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCa                                                                 <  2:470634/151‑1 (MQ=255)

                                                                                   aGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGt                                                             <  1:311589/151‑1 (MQ=255)

                                                                                       gTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAAc                                                          <  2:510024/150‑1 (MQ=255)

                                                                                          cTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTg                                                      >  2:504434/1‑151 (MQ=255)

                                                                                               aTCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTg                                                 >  1:509573/1‑151 (MQ=255)

                                                                                                   cATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGaa                                               <  2:667043/149‑1 (MQ=255)

                                                                                                   cATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAAt                                              <  2:660384/150‑1 (MQ=255)

                                                                                                      tGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGa                                           <  1:58681/150‑1 (MQ=255)

                                                                                                       gACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGAc                                          <  1:548695/150‑1 (MQ=255)

                                                                                                       gACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACa                                         <  1:68812/151‑1 (MQ=255)

                                                                                                            tCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGaa                                      <  2:418758/149‑1 (MQ=255)

                                                                                                            tCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGaa                                      <  1:468855/149‑1 (MQ=255)

                                                                                                            tCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAAc                                     <  2:41392/150‑1 (MQ=255)

                                                                                                               ggCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACg                                    <  1:324897/148‑1 (MQ=255)

                                                                                                               ggCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGa                                   <  2:172087/149‑1 (MQ=255)

                                                                                                               ggCCCTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAAt                                 <  2:501690/151‑1 (MQ=255)

                                                                                                                  ccTTGGTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCg                               <  1:532560/150‑1 (MQ=255)

                                                                                                                      ggTGATCATCAGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACt                           <  1:659738/150‑1 (MQ=255)

                                                                                                                             atcaGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACtt                   <  2:40658/151‑1 (MQ=255)

                                                                                                                               caGCATAATCTGCAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTc                  <  2:296301/150‑1 (MQ=255)

                                                                                                                                           cAATTCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCcgcg     <  2:400984/151‑1 (MQ=255)

                                                                                                                                               tCACGCTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCCGCGCCt  <  1:65769/150‑1 (MQ=255)

GAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGCTGCCCTGAATAGACAGAACGAATCGCACTCACGACTTCCTGCGGAGCCGCGCCT  >  NC_000913/1994756‑1995048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A