Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,290	T→C	S36P (TCT→CCT)	yegJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,290	0	T	C	100.0%	99.4 / NA	28	S36P (TCT→CCT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/6); total (22/6)

GTTTTTGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGTCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCT  >  NC_000913/2151144‑2151436

gTTTTTGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTg                                                                                                                                                 >  1:267237/1‑150 (MQ=255)

  ttttGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGAc                                                                                                                                               <  1:292401/150‑1 (MQ=255)

               gACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATg                                                                                                                                  <  2:247366/150‑1 (MQ=255)

                   aCAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCgg                                                                                                                             >  2:79440/1‑151 (MQ=255)

                   aCAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCgg                                                                                                                             <  2:572832/151‑1 (MQ=255)

                     aaaGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGga                                                                                                                            >  2:458496/1‑150 (MQ=255)

                       aGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGgaa                                                                                                                           >  1:275269/1‑149 (MQ=255)

                              cTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCg                                                                                                                   >  1:265179/1‑150 (MQ=255)

                               tGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGAt                                                                                                                 >  2:467462/1‑151 (MQ=255)

                                 gagaAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATa                                                                                                                >  2:132459/1‑150 (MQ=255)

                                   gaAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAAt                                                                                                             >  2:222827/1‑151 (MQ=255)

                                    aaaaaaTGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAAtt                                                                                                            >  2:210735/1‑151 (MQ=255)

                                       aaaTGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCAt                                                                                                         >  1:319541/1‑151 (MQ=255)

                                        aaTGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATc                                                                                                        >  2:630855/1‑151 (MQ=255)

                                            aaaaaGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAgg                                                                                                    >  2:543865/1‑151 (MQ=255)

                                                 gATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATg                                                                                               >  1:336598/1‑151 (MQ=255)

                                                  atatTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATg                                                                                               >  1:532245/1‑150 (MQ=255)

                                                     ttACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAAt                                                                                            >  2:241608/1‑150 (MQ=255)

                                                      tACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATa                                                                                           >  2:602371/1‑150 (MQ=255)

                                                      tACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATa                                                                                           >  1:594008/1‑150 (MQ=255)

                                                           aTGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATaaaaaa                                                                                      <  1:176544/150‑1 (MQ=255)

                                                                     tGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATAc                                                                           >  1:317790/1‑151 (MQ=255)

                                                                                    ccGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGc                                                            <  1:15800/151‑1 (MQ=255)

                                                                                         ggCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGa                                                       <  2:28948/151‑1 (MQ=255)

                                                                                                            aTACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAAc                                    >  1:28689/1‑151 (MQ=255)

                                                                                                                       aCGGGAGGTTATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCt                         >  1:259222/1‑151 (MQ=255)

                                                                                                                               ttATGATAAACAAGCGATGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACg                 >  2:162279/1‑151 (MQ=255)

                                                                                                                                               aTGCCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCt  >  2:650654/1‑150 (MQ=255)

GTTTTTGCTAACTTTGACTACAAAGGGATTCTGGAGAAAAAATGAAAAAGATATTACTTATGTTGAGTCTGCTATTTTTTACTACCGCGGGCTTTAGTGAAGTTTCGGATACGTTAGTGACGGGAGGTTATGATAAACAAGCGATGTCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCT  >  NC_000913/2151144‑2151436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A