Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,009	T→C	T100A (ACA→GCA)	yegR ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,009	0	T	C	100.0%	125.3 / NA	33	T100A (ACA→GCA)	yegR	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/23); total (10/23)

TGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAA  >  NC_000913/2167866‑2168151

tGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACg                                                                                                                                          >  1:151552/1‑150 (MQ=255)

   ccACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCg                                                                                                                                      <  1:58692/151‑1 (MQ=255)

               aaTCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAAt                                                                                                                          >  1:134041/1‑151 (MQ=255)

               aaTCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAAt                                                                                                                          <  1:430981/151‑1 (MQ=255)

                       ttCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACAcc                                                                                                                  >  1:647245/1‑151 (MQ=255)

                         cATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACGcc                                                                                                                  <  2:15060/149‑1 (MQ=255)

                            aGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCt                                                                                                              <  1:339224/150‑1 (MQ=255)

                             gCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGt                                                                                                            <  1:51050/151‑1 (MQ=255)

                               aCGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCt                                                                                                          >  1:77269/1‑151 (MQ=255)

                                        ttCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATAGTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCa                                                                                                  <  2:558032/150‑1 (MQ=255)

                                             ttATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTAtt                                                                                            >  2:525941/1‑151 (MQ=255)

                                                  ggCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAt                                                                                       <  1:26608/151‑1 (MQ=255)

                                                    cATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAttt                                                                                     <  2:571042/151‑1 (MQ=255)

                                                      tCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAttttt                                                                                   >  1:274520/1‑151 (MQ=255)

                                                      tCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCAttttt                                                                                   >  2:105915/1‑151 (MQ=255)

                                                             cccACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGccc                                                                             <  1:443957/150‑1 (MQ=255)

                                                                 caGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGt                                                                        <  2:158123/151‑1 (MQ=255)

                                                                           cTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGaa                                                               >  1:305080/1‑150 (MQ=255)

                                                                             aCGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATg                                                            <  2:638816/151‑1 (MQ=255)

                                                                                   aGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATACTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTg                                                       <  2:274449/150‑1 (MQ=255)

                                                                                     ccAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGcat                                                    <  1:203576/151‑1 (MQ=255)

                                                                                       aGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGcatca                                                  <  1:631483/151‑1 (MQ=255)

                                                                                          aCGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCAttt                                               <  1:273853/151‑1 (MQ=255)

                                                                                            gcgcATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCAttttt                                             <  1:397173/151‑1 (MQ=255)

                                                                                              gcATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCAtttttt                                            >  1:559138/1‑150 (MQ=255)

                                                                                                            tACCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTg                               <  2:4824/149‑1 (MQ=255)

                                                                                                             aCCAAAAAATATTCTCACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCa                             <  1:555029/150‑1 (MQ=255)

                                                                                                                         tctcACTCTCCACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTa                 <  1:174580/150‑1 (MQ=255)

                                                                                                                                  ccACATTTTAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCt        <  2:583082/150‑1 (MQ=255)

                                                                                                                                  ccACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGc         <  1:522158/149‑1 (MQ=255)

                                                                                                                                  ccACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCt        >  1:269871/1‑150 (MQ=255)

                                                                                                                                  ccACATTTGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTa       <  1:354460/151‑1 (MQ=255)

                                                                                                                                        ttGAATGCCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAaagaa  <  2:630074/150‑1 (MQ=255)

TGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAA  >  NC_000913/2167866‑2168151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A