Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,261,739	T→C	A209A (GCA→GCG)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,739	0	T	C	100.0%	148.7 / NA	40	A209A (GCA→GCG)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/18); total (22/18)

CCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACA  >  NC_000913/2261605‑2261885

ccTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACAt                                                                                                                                    >  1:629503/1‑151 (MQ=255)

   gcgcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATc                                                                                                                                   <  1:374287/149‑1 (MQ=255)

     gcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttc                                                                                                                               >  2:109370/1‑151 (MQ=255)

     gcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttc                                                                                                                               <  1:310773/151‑1 (MQ=255)

       cAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttcat                                                                                                                             >  2:307257/1‑151 (MQ=255)

         aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttcattt                                                                                                                           >  2:452206/1‑151 (MQ=255)

           ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttcatttca                                                                                                                         <  2:284414/151‑1 (MQ=255)

                 tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCa                                                                                                                     <  2:343869/149‑1 (MQ=255)

                 tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAg                                                                                                                    <  1:479867/150‑1 (MQ=255)

                 tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGt                                                                                                                   >  1:369342/1‑151 (MQ=255)

                  aCGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGtt                                                                                                                  >  1:622276/1‑151 (MQ=255)

                  aCGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGtt                                                                                                                  >  1:227218/1‑151 (MQ=255)

                   cGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGttt                                                                                                                 >  1:445483/1‑151 (MQ=255)

                      cATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACg                                                                                                              >  1:115828/1‑151 (MQ=255)

                              gACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGcc                                                                                                       <  1:29457/150‑1 (MQ=255)

                                       ttGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTcc                                                                                              <  1:223339/150‑1 (MQ=255)

                                              tCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTcgcg                                                                                      >  1:622681/1‑151 (MQ=255)

                                                   tCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGcggcgg                                                                                  <  2:154952/150‑1 (MQ=255)

                                                    cGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGtt                                                                                <  1:229106/151‑1 (MQ=255)

                                                      ccGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGtt                                                                                >  1:581590/1‑149 (MQ=255)

                                                      ccGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAg                                                                              <  1:521150/151‑1 (MQ=255)

                                                          aGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGttt                                                                          >  2:366058/1‑151 (MQ=255)

                                                                    ccAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCa                                                                 >  1:160344/1‑150 (MQ=255)

                                                                               cTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCttt                                                     >  1:290656/1‑151 (MQ=255)

                                                                                 tCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCttt                                                     <  1:528422/149‑1 (MQ=255)

                                                                                  cGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCaa                                                  <  2:425348/151‑1 (MQ=255)

                                                                                     cACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAAcc                                               <  1:481691/151‑1 (MQ=255)

                                                                                        ccAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGc                                            <  1:503308/151‑1 (MQ=255)

                                                                                         cAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGct                                           <  2:365632/151‑1 (MQ=255)

                                                                                           gTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGctac                                         <  1:495142/151‑1 (MQ=255)

                                                                                                aTAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTATGATTCACCACCCACCGGGCCGCTTTCACACCTGCTCCTaa                                      >  1:349111/1‑149 (MQ=255)

                                                                                                  aacaacATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCt                                  >  2:6722/1‑151 (MQ=255)

                                                                                                   acaacaTGCGCGATGCATTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAAGTTTCACCAGGCAAGGGGGCGCTTTTAAAACTGCTTCCAACACtt                                 >  2:131134/1‑151 (MQ=255)

                                                                                                       caTGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGGTTCACCAGCCACGGGGCCGCTTTCAAACCCGCTACTAACGCTTCa                               >  2:415104/1‑149 (MQ=255)

                                                                                                          gcgcGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGc                           <  2:316412/150‑1 (MQ=255)

                                                                                                                  ccTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCCCCCGCCACGGTGCCGCTTTCAAACCTGGTACTAACGGTTCAACGctact                       >  1:86228/1‑146 (MQ=255)

                                                                                                                     tGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAg                <  1:527347/150‑1 (MQ=255)

                                                                                                                              aGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGGTTTACCAGCCACGGTGCCGCCTTCAAACCTGCTACTAAAGCTTCACGGCCACTATCAAAAATAATGCaa       >  1:302209/1‑150 (MQ=255)

                                                                                                                                 gcATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGCTAGGTTTCAACAACCACGGTGCCGCCTTCAAACCTGCTACTAACGCTTCCCGGACAACATTAAAGATAATTGAAgg     >  2:160540/1‑149 (MQ=255)

                                                                                                                                   aTGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCTTTTCAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAAAGCAAAGaca  >  2:365227/1‑150 (MQ=255)

CCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACA  >  NC_000913/2261605‑2261885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A