Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,325,637 T→C R294R (CGT→CGC)  atoE → short chain fatty acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,325,6370TC100.0% 140.8 / NA 41R294R (CGT→CGC) atoEshort chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

TCTGACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGTGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCG  >  NC_000913/2325490‑2325784
                                                                                                                                                   |                                                                                                                                                   
tCTGACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATgcgcgc                                                                                                                                                   <  2:286914/150‑1 (MQ=255)
   gACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATc                                                                                                                                               <  2:502745/151‑1 (MQ=255)
         gATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGcgcg                                                                                                                                         <  2:49812/151‑1 (MQ=255)
              tcGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGgcagc                                                                                                                                    >  1:317300/1‑151 (MQ=255)
                     aCTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGt                                                                                                                             <  2:223252/151‑1 (MQ=255)
                       taggTATCGCTTACCTTGCGATGAACTTCAGCGAACATGGCTTCAACACCACCATCGATACAGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAATCGCCACCGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGt                                                                                                                             >  2:431370/3‑149 (MQ=255)
                                    ccTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTg                                                                                                              <  1:644519/151‑1 (MQ=255)
                                    ccTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTg                                                                                                              >  1:413312/1‑151 (MQ=255)
                                        gCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGc                                                                                                          <  2:391804/151‑1 (MQ=255)
                                          gATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCa                                                                                                         >  1:11639/1‑150 (MQ=255)
                                                  tCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCt                                                                                                 <  1:286752/150‑1 (MQ=255)
                                                   cAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCtt                                                                                                <  1:619499/150‑1 (MQ=255)
                                                     gCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTa                                                                                             <  2:558922/151‑1 (MQ=255)
                                                             ggCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTggg                                                                                      >  1:206394/1‑150 (MQ=255)
                                                                   aaCATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAAc                                                                               >  1:575023/1‑151 (MQ=255)
                                                                    aCATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAAc                                                                               >  1:474168/1‑150 (MQ=255)
                                                                     cATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACtg                                                                             <  1:384224/151‑1 (MQ=255)
                                                                        cACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACtgatg                                                                          <  1:601393/151‑1 (MQ=255)
                                                                           cATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGatgatg                                                                       >  1:55853/1‑151 (MQ=255)
                                                                               aaTACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGc                                                                   >  2:457601/1‑151 (MQ=255)
                                                                                   ccGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTc                                                               <  1:537181/151‑1 (MQ=255)
                                                                                         aCCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCt                                                         >  2:582450/1‑151 (MQ=255)
                                                                                             gATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTgg                                                       <  2:526855/149‑1 (MQ=255)
                                                                                              aTGTTGATGATTGCGGGTCTGCTGCTGCATAAAACGCCAATGGCTTATATGCGCGCTGTCAGCGCGGGTGCAGGCGGTACTGCCGGTATTCTGGTGCAATTCCCCTTCGGCGCTGGGATCCAACTGATGATGGAGCATTCCGGTcg                                                         <  2:462963/146‑2 (MQ=255)
                                                                                                 tttATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGAc                                                 >  1:100828/1‑151 (MQ=255)
                                                                                                    aTGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTc                                               >  2:87132/1‑150 (MQ=255)
                                                                                                       cttGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGAATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGACGATGGAGCATTCCGGCCTGGGCGGACTCAtt                                            >  2:386208/2‑150 (MQ=255)
                                                                                                           cGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCg                                        >  2:661658/1‑150 (MQ=255)
                                                                                                             ggTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAt                                     >  1:288091/1‑151 (MQ=255)
                                                                                                              gTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAtt                                    <  1:221842/151‑1 (MQ=255)
                                                                                                                 tgctgcTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAttctt                                 >  1:253647/1‑151 (MQ=255)
                                                                                                                   ctgctACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAttcttc                                >  2:635557/1‑150 (MQ=255)
                                                                                                                         cATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATg                         >  1:295024/1‑151 (MQ=255)
                                                                                                                         cATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATg                         >  2:184573/1‑151 (MQ=255)
                                                                                                                           tAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGtt                       >  1:581263/1‑151 (MQ=255)
                                                                                                                                cGCCAAGGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGaa                  <  2:139355/151‑1 (MQ=255)
                                                                                                                                 gCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAAc                 <  1:506173/151‑1 (MQ=255)
                                                                                                                                  ccAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACa                <  1:414814/151‑1 (MQ=255)
                                                                                                                                  ccAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACa                <  2:173571/151‑1 (MQ=255)
                                                                                                                                   cAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACaa               <  2:12622/151‑1 (MQ=255)
                                                                                                                                       ggCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGa            <  2:462522/150‑1 (MQ=255)
                                                                                                                                          ttATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACAcc        >  1:227997/1‑151 (MQ=255)
                                                                                                                                                gcgcgcTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCg  <  2:600280/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCTGACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGTGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCG  >  NC_000913/2325490‑2325784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A