Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,241	T→A	N7Y (AAC→TAC)	menD ←	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,241	0	T	A	100.0%	212.3 / NA	53	N7Y (AAC→TAC)	menD	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (24/29); total (24/29)

TCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTT  >  NC_000913/2379096‑2379386

tCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATg                                                                                                                                              >  1:628080/1‑151 (MQ=255)

 cATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAAt                                                                                                                                               <  1:188640/149‑1 (MQ=255)

  aTCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATgcg                                                                                                                                            >  1:71485/1‑151 (MQ=255)

     gAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCtt                                                                                                                                         >  2:476989/1‑151 (MQ=255)

              gtggtgAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATg                                                                                                                                <  2:604502/151‑1 (MQ=255)

                    aatgaatgCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTg                                                                                                                          >  1:262446/1‑151 (MQ=255)

                         atgCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCgg                                                                                                                     >  1:598138/1‑151 (MQ=255)

                          tgCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGt                                                                                                                    >  1:663535/1‑151 (MQ=255)

                            cGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTAt                                                                                                                  >  2:479104/1‑151 (MQ=255)

                               aaTTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATACGCTTACTGACATGAGTTTGTCCGGTATCa                                                                                                                >  2:371831/1‑150 (MQ=255)

                                aTTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAat                                                                                                              <  2:612457/151‑1 (MQ=255)

                                     ccgccgccgCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGc                                                                                                         <  2:295291/151‑1 (MQ=255)

                                      cgccgccgcTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCg                                                                                                        <  1:517121/151‑1 (MQ=255)

                                            cgcTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTa                                                                                                    >  1:537026/1‑149 (MQ=255)

                                             gcTAACGTTAACGGGGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGt                                                                                                 >  1:263370/1‑151 (MQ=255)

                                                aaCGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGtat                                                                                               <  1:434291/150‑1 (MQ=255)

                                                aaCGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGtat                                                                                               <  2:75482/150‑1 (MQ=255)

                                                  cGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAg                                                                                            <  1:7197/151‑1 (MQ=255)

                                                   gTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAgg                                                                                           >  1:320935/1‑151 (MQ=255)

                                                      aaCGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAg                                                                                         <  1:343770/150‑1 (MQ=255)

                                                         ggTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTAc                                                                                     >  1:49906/1‑151 (MQ=255)

                                                                    cgAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGatt                                                                          <  2:266691/151‑1 (MQ=255)

                                                                          tGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGat                                                                    >  1:501468/1‑151 (MQ=255)

                                                                           ggggCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGat                                                                    <  1:154767/150‑1 (MQ=255)

                                                                              gCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGa                                                                <  2:259750/151‑1 (MQ=255)

                                                                               cGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGaa                                                               <  2:357806/151‑1 (MQ=255)

                                                                                  tACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATc                                                             <  2:16994/150‑1 (MQ=255)

                                                                                     aGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGt                                                          >  1:205014/1‑150 (MQ=255)

                                                                                     aGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGt                                                          >  1:424137/1‑150 (MQ=255)

                                                                                           gtCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAatgatg                                                   <  2:443910/151‑1 (MQ=255)

                                                                                              tGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCg                                                 >  1:172204/1‑150 (MQ=255)

                                                                                                 cGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTc                                             <  2:109874/151‑1 (MQ=255)

                                                                                                 cGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTc                                             >  1:347485/1‑151 (MQ=255)

                                                                                                 cGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTc                                             <  1:11800/151‑1 (MQ=255)

                                                                                                  gCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTc                                             >  2:667505/1‑150 (MQ=255)

                                                                                                      tGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTAc                                        >  2:624954/1‑151 (MQ=255)

                                                                                                       gACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACt                                       <  1:214953/151‑1 (MQ=255)

                                                                                                        aCGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTa                                      <  2:292343/151‑1 (MQ=255)

                                                                                                           cgTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTc                                   <  2:69583/151‑1 (MQ=255)

                                                                                                            gTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTcc                                  >  1:415933/1‑151 (MQ=255)

                                                                                                              tAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCAt                                >  2:84806/1‑151 (MQ=255)

                                                                                                                  gCTTCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTg                             <  1:405334/150‑1 (MQ=255)

                                                                                                                     tCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaat                         >  1:150482/1‑151 (MQ=255)

                                                                                                                     tCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaa                          <  1:299986/150‑1 (MQ=255)

                                                                                                                     tCCAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGta                           <  2:71483/149‑1 (MQ=255)

                                                                                                                      ccAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaata                        <  2:561671/151‑1 (MQ=255)

                                                                                                                      ccAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaata                        <  1:579291/151‑1 (MQ=255)

                                                                                                                      ccAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaat                         >  2:77210/1‑150 (MQ=255)

                                                                                                                       cAGAATGACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGtaataa                       <  1:562578/151‑1 (MQ=255)

                                                                                                                             gACCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTAc                  <  1:630849/150‑1 (MQ=255)

                                                                                                                              aCCGCCGCCCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGc                >  2:491160/1‑151 (MQ=255)

                                                                                                                                  ccgccCAGCGTCGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGcc            <  2:188819/151‑1 (MQ=255)

                                                                                                                                             cGGTAAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCttt  <  1:386056/150‑1 (MQ=255)

TCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTCCAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTT  >  NC_000913/2379096‑2379386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A