Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,441,945	T→C	V61A (GTG→GCG)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,441,945	0	T	C	100.0%	189.9 / NA	52	V61A (GTG→GCG)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/25); total (27/25)

TTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTT  >  NC_000913/2441801‑2442092

ttAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAg                                                                                                                                                <  1:400018/150‑1 (MQ=255)

 tAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGc                                                                                                                                               <  1:170188/150‑1 (MQ=255)

        gAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGCTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCg                                                                                                                                       <  2:452582/151‑1 (MQ=255)

        gAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCAATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCg                                                                                                                                       <  1:492816/151‑1 (MQ=255)

               cacCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCgg                                                                                                                                >  2:291942/1‑151 (MQ=255)

                acCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGc                                                                                                                               >  2:542824/1‑151 (MQ=255)

                acCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGc                                                                                                                               <  1:190008/151‑1 (MQ=255)

                     tttCCCGAGATTTTGACGATGTCTATTTTTCCAACCATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCgg                                                                                                                          >  1:59639/1‑151 (MQ=255)

                      ttCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCgg                                                                                                                          >  1:365969/1‑150 (MQ=255)

                          cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTaa                                                                                                                     <  1:423658/151‑1 (MQ=255)

                          cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTa                                                                                                                      >  2:465553/1‑150 (MQ=255)

                          cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTa                                                                                                                      >  2:26013/1‑150 (MQ=255)

                           gagaTTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTaa                                                                                                                     >  2:90842/1‑150 (MQ=255)

                              aTTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACtt                                                                                                                 <  2:218220/151‑1 (MQ=255)

                                            tATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggc                                                                                                   >  2:168116/1‑151 (MQ=255)

                                             aTTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggca                                                                                                  <  1:419026/151‑1 (MQ=255)

                                             aTTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggc                                                                                                   <  1:16841/150‑1 (MQ=255)

                                               ttttCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggcagg                                                                                                >  2:95856/1‑151 (MQ=255)

                                                   cgaaCGATAACGGGCTGGCAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCAtt                                                                                            >  2:360707/3‑151 (MQ=255)

                                                              gggCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTc                                                                                 >  2:92319/1‑151 (MQ=255)

                                                               ggCTGGAAGAGACGCGTTATGTTTTTCTGGGACGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAGCTTCCTGACGCTATGGCAGGCATTTGATCAGTTTcg                                                                                <  2:61308/151‑1 (MQ=255)

                                                                       gagaCGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAgcgc                                                                        <  1:180792/151‑1 (MQ=255)

                                                                          aCGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATc                                                                     >  2:285425/1‑151 (MQ=255)

                                                                                 aTGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgc                                                              <  1:544625/151‑1 (MQ=255)

                                                                                 aTGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgc                                                              >  2:158890/1‑151 (MQ=255)

                                                                                   gTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgcgc                                                            >  1:591393/1‑151 (MQ=255)

                                                                                    tttttCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgcgc                                                            >  2:388256/1‑150 (MQ=255)

                                                                                           gggAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACaa                                                    <  2:672089/151‑1 (MQ=255)

                                                                                           gggAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACa                                                     <  1:26492/150‑1 (MQ=255)

                                                                                              aGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGc                                                 <  1:381424/151‑1 (MQ=255)

                                                                                              aGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGc                                                 <  1:296937/151‑1 (MQ=255)

                                                                                               ggCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCt                                                <  2:371818/151‑1 (MQ=255)

                                                                                                gCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCtt                                               >  2:458813/1‑151 (MQ=255)

                                                                                                 cAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCtt                                               >  2:398221/1‑150 (MQ=255)

                                                                                                 cAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTa                                              <  1:348437/151‑1 (MQ=255)

                                                                                                  aaCCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTAc                                             <  2:386374/151‑1 (MQ=255)

                                                                                                      aaTTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACAttt                                         >  1:471227/1‑151 (MQ=255)

                                                                                                       cttAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACAttt                                         >  2:162289/2‑150 (MQ=255)

                                                                                                        ttAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTc                                        >  1:348131/1‑150 (MQ=255)

                                                                                                          agagGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCAtt                                     >  1:637511/1‑151 (MQ=255)

                                                                                                            agGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCAtt                                     >  2:180337/1‑149 (MQ=255)

                                                                                                            agGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAg                                   <  1:639679/151‑1 (MQ=255)

                                                                                                             ggTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGt                                  >  1:359449/1‑151 (MQ=255)

                                                                                                              gTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGtt                                 <  2:163878/151‑1 (MQ=255)

                                                                                                                aCGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGtttt                               <  1:193939/151‑1 (MQ=255)

                                                                                                                       ccTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGaaa                         <  2:402471/150‑1 (MQ=255)

                                                                                                                             cATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTcccc                  >  1:285218/1‑151 (MQ=255)

                                                                                                                                ccACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCa               <  1:435062/151‑1 (MQ=255)

                                                                                                                                 cacaTCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCAc              >  2:372550/1‑151 (MQ=255)

                                                                                                                                    aTCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCg           <  2:618118/151‑1 (MQ=255)

                                                                                                                                          tGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCgg      >  2:218952/1‑150 (MQ=255)

                                                                                                                                             ttGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGAttt  >  2:588516/1‑151 (MQ=255)

TTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTT  >  NC_000913/2441801‑2442092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A