Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,595,335	T→C	A175A (GCA→GCG)	tmcA ←	elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,595,335	0	T	C	100.0%	182.2 / NA	50	A175A (GCA→GCG)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/19); total (31/19)

GCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTC  >  NC_000913/2595187‑2595478

gcgcGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGgcgc                                                                                                                                               <  1:44719/151‑1 (MQ=255)

       gACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGt                                                                                                                                         <  1:536124/150‑1 (MQ=255)

        aCAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTcg                                                                                                                                       <  2:294863/151‑1 (MQ=255)

                  tGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTAcc                                                                                                                              >  2:621843/1‑150 (MQ=255)

                   gCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTAcc                                                                                                                              >  2:355279/1‑149 (MQ=255)

                   gCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACca                                                                                                                             <  1:118948/150‑1 (MQ=255)

                    cccGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagt                                                                                                                           >  1:51519/1‑151 (MQ=255)

                    cccGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagt                                                                                                                           <  2:43373/151‑1 (MQ=255)

                       gCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagtcag                                                                                                                        <  2:360864/151‑1 (MQ=255)

                         aaTACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagtcagt                                                                                                                       >  1:310810/1‑150 (MQ=255)

                           tACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACg                                                                                                                    >  1:18807/1‑151 (MQ=255)

                             cGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACggg                                                                                                                  >  1:574208/1‑151 (MQ=255)

                              gagaAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACgggg                                                                                                                 >  1:292296/1‑151 (MQ=255)

                              gagaAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACggg                                                                                                                  >  2:297932/1‑150 (MQ=255)

                               agaAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGa                                                                                                                <  2:32348/151‑1 (MQ=255)

                                  aaTGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGt                                                                                                              >  1:665058/1‑150 (MQ=255)

                                   aTGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTa                                                                                                             >  2:48091/1‑150 (MQ=255)

                                     gAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTaaaa                                                                                                          <  2:524149/151‑1 (MQ=255)

                                         tGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATgcg                                                                                                      >  2:274775/1‑151 (MQ=255)

                                             cTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAGAATGCGcc                                                                                                    >  2:456514/1‑149 (MQ=255)

                                                 cAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCTCCAACgag                                                                                              <  2:93431/151‑1 (MQ=255)

                                                    cGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGaa                                                                                            >  1:248681/1‑150 (MQ=255)

                                                        gACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCt                                                                                       >  1:109528/1‑151 (MQ=255)

                                                        gACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCt                                                                                       >  1:526629/1‑151 (MQ=255)

                                                            tACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTgg                                                                                     <  2:646382/149‑1 (MQ=255)

                                                            tACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGtt                                                                                   >  1:421204/1‑151 (MQ=255)

                                                              ccGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGtttt                                                                                 <  1:570446/151‑1 (MQ=255)

                                                               cgcgCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGtttt                                                                                 >  2:393443/1‑150 (MQ=255)

                                                                      aCGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTgccgcc                                                                           >  2:528263/1‑149 (MQ=255)

                                                                          gcAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAgg                                                                      >  2:625146/1‑150 (MQ=255)

                                                                                gTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCt                                                               >  2:405944/1‑151 (MQ=255)

                                                                                      gCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTAt                                                         <  2:148840/151‑1 (MQ=255)

                                                                                      gCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTAt                                                         <  2:433244/151‑1 (MQ=255)

                                                                                         gCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATccg                                                      <  2:131526/151‑1 (MQ=255)

                                                                                         gCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATcc                                                       <  1:467017/150‑1 (MQ=255)

                                                                                                ccGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTa                                                 >  1:570809/1‑149 (MQ=255)

                                                                                                         tGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGttt                                      >  2:210587/1‑151 (MQ=255)

                                                                                                          ggTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTg                                     >  1:230540/1‑151 (MQ=255)

                                                                                                             cATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTg                                     >  1:39682/1‑148 (MQ=255)

                                                                                                                tAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGc                               >  2:196155/1‑151 (MQ=255)

                                                                                                                 aGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCt                              >  1:315053/1‑151 (MQ=255)

                                                                                                                     gctTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGAc                          >  2:164151/1‑151 (MQ=255)

                                                                                                                         tAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACaaa                       <  1:391734/150‑1 (MQ=255)

                                                                                                                          aaGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAAt                     >  2:248454/1‑151 (MQ=255)

                                                                                                                             agTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATgcg                  <  1:448162/151‑1 (MQ=255)

                                                                                                                              gTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATgcgg                 <  2:475545/151‑1 (MQ=255)

                                                                                                                                  cTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTc             >  2:176478/1‑151 (MQ=255)

                                                                                                                                         tCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCCAt        >  2:577362/1‑149 (MQ=255)

                                                                                                                                           tGGTTGTGGCGCGCCAGTCGCGGGGTACCAGCCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg    >  2:91073/1‑151 (MQ=255)

                                                                                                                                            ggTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGt   <  1:634358/151‑1 (MQ=255)

                                                                                                                                             gTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTc  <  2:73599/151‑1 (MQ=255)

GCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTC  >  NC_000913/2595187‑2595478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A