Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,250	C→T	V108I (GTA→ATA)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,250	0	C	T	100.0%	212.5 / NA	55	V108I (GTA→ATA)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/32); total (23/32)

CCGCTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCT  >  NC_000913/649105‑649393

ccgcTCTGTCCCAGTTGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcg                                                                                                                                            <  1:202672/151‑1 (MQ=255)

  gcTCTGTACCAAAAGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcggc                                                                                                                                          <  1:680880/151‑1 (MQ=255)

   ctctGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAgcggcg                                                                                                                                         >  1:56642/1‑151 (MQ=255)

    tctGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGgcgc                                                                                                                                        >  1:590091/1‑151 (MQ=255)

         aCCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGc                                                                                                                                   >  1:245636/1‑151 (MQ=255)

          ccAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATgg                                                                                                                                    <  1:766682/149‑1 (MQ=255)

             gATGCACACGCCCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCa                                                                                                                                  <  2:241152/148‑1 (MQ=255)

                 cacaCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCa                                                                                                                              <  1:889204/148‑1 (MQ=255)

                 cacaCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCAc                                                                                                                             <  2:78261/149‑1 (MQ=255)

                    acGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAgg                                                                                                                        >  2:187800/1‑151 (MQ=255)

                     gggcggccgAGAGAGTGGATCAGCACCGTTTCGGCCTGCAGACCACGGGAGATTTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAAGCTCGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAg                                                                                                                         <  2:554568/144‑1 (MQ=255)

                        ccgccgTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGa                                                                                                                      <  2:318965/149‑1 (MQ=255)

                           ccgTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTgg                                                                                                                 <  2:398571/151‑1 (MQ=255)

                             gTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGAc                                                                                                               <  1:234690/151‑1 (MQ=255)

                              tGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACg                                                                                                              >  1:458906/1‑151 (MQ=255)

                               gagaGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACg                                                                                                              <  1:671234/150‑1 (MQ=255)

                                     ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAggg                                                                                                        >  2:1044769/1‑150 (MQ=255)

                                     ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGt                                                                                                       >  2:18583/1‑151 (MQ=255)

                                     ggATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGt                                                                                                       >  2:457926/1‑151 (MQ=255)

                                            cACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttt                                                                                                >  2:123640/1‑151 (MQ=255)

                                              ccGGTTCTGCCAGCCGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGCATACATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttttt                                                                                              >  2:133921/1‑151 (MQ=255)

                                              ccGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGttttt                                                                                              >  1:108315/1‑151 (MQ=255)

                                                ggTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTaa                                                                                            <  2:69717/151‑1 (MQ=255)

                                                 gTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTaaa                                                                                           <  2:382898/151‑1 (MQ=255)

                                                 gTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTaa                                                                                            >  1:670583/1‑150 (MQ=255)

                                                   tCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAg                                                                                          <  1:39344/150‑1 (MQ=255)

                                                         agcagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCt                                                                                   <  2:566792/151‑1 (MQ=255)

                                                           cagACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTc                                                                                 <  2:694183/151‑1 (MQ=255)

                                                             gACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTcgc                                                                               <  1:437510/151‑1 (MQ=255)

                                                              aCCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTcgcg                                                                              >  2:374315/1‑151 (MQ=255)

                                                               ccACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGa                                                                             <  2:173916/151‑1 (MQ=255)

                                                                 aCGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTGTCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATg                                                                           <  1:184272/151‑1 (MQ=255)

                                                                 aCGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATg                                                                           <  1:285657/151‑1 (MQ=255)

                                                                            ctTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGG‑TTTTAAAGCCCATCTTCGCGATGACGTCCATc                                                                  <  2:413428/148‑1 (MQ=255)

                                                                               ccGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCat                                                             >  1:20147/1‑151 (MQ=255)

                                                                                  ccAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATAtt                                                          <  2:376379/151‑1 (MQ=255)

                                                                                   cAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTg                                                         >  1:602561/1‑151 (MQ=255)

                                                                                     gTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGAc                                                       >  2:472941/1‑151 (MQ=255)

                                                                                       ggACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACg                                                      <  2:411680/150‑1 (MQ=255)

                                                                                             cGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtcaggt                                               <  2:481241/151‑1 (MQ=255)

                                                                                             cGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACggtcaggt                                               >  1:1059544/1‑151 (MQ=255)

                                                                                                  gCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCg                                          >  1:982145/1‑151 (MQ=255)

                                                                                                    cGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGcc                                        >  1:779899/1‑151 (MQ=255)

                                                                                                        ggTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCAGGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACg                                     <  2:62614/150‑1 (MQ=255)

                                                                                                         gTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGa                                   <  1:901422/151‑1 (MQ=255)

                                                                                                           ataAATGCGGGTACCCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGaa                                  <  2:771555/150‑1 (MQ=255)

                                                                                                             aaaTGCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGATCACCGCCACGGAAAg                                <  2:1019504/150‑1 (MQ=255)

                                                                                                                 gCGGGTAACCACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTg                            <  1:261214/150‑1 (MQ=255)

                                                                                                                       aaacACGCCCGGGCGAATGTGTTCTATCAGCGGCGAATGGCAACCACTCAGGGAGCTGGAGGCCAGGGTCAGGTTTTTAAAGCCCATCTCCGCGATGACGTCAATCAACAAATTGGCGGTCAGGTCGCCGCCACGGAAAGCGTGATggaag                     <  2:419019/148‑1 (MQ=255)

                                                                                                                          cACGCCCTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATggaaggaa                  <  2:72607/151‑1 (MQ=255)

                                                                                                                               ccTGGCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGt             <  2:393144/151‑1 (MQ=255)

                                                                                                                                   gCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATg         >  2:303109/1‑151 (MQ=255)

                                                                                                                                   gCGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATg         >  1:422994/1‑151 (MQ=255)

                                                                                                                                    cGAATGTGTTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGc        >  2:460091/1‑151 (MQ=255)

                                                                                                                                          tgtTCTATCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCt  >  1:546866/1‑151 (MQ=255)

CCGCTCTGTACCAGATGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTCTTCCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATGTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCT  >  NC_000913/649105‑649393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A