Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 160 117.2 4331776 85.4% 3699336 149.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,527,479 G→A G178G (GGG→GGA)  yfeH → putative inorganic ion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,527,4790GA98.1% 394.6 / ‑4.5 103G178G (GGG→GGA) yfeHputative inorganic ion transporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (52/49);  total (54/49)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GGTTTGTTCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGG  >  NC_000913/2527332‑2527625
                                                                                                                                                   |                                                                                                                                                  
ggTTTGTTCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACAt                                                                                                                                                 >  3:381149/1‑151 (MQ=255)
 gTTTGTTCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATc                                                                                                                                                <  3:342023/151‑1 (MQ=255)
    tGTTCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCtt                                                                                                                                              <  3:182973/150‑1 (MQ=255)
     gTTCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTc                                                                                                                                            <  4:102394/151‑1 (MQ=255)
       tCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTcc                                                                                                                                           <  4:1357121/150‑1 (MQ=255)
          gCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCgg                                                                                                                                        <  2:1362798/150‑1 (MQ=255)
          gCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCg                                                                                                                                         <  4:1278465/149‑1 (MQ=255)
          gCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGc                                                                                                                                       <  2:1593819/151‑1 (MQ=255)
           cGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGcc                                                                                                                                      <  4:1630465/151‑1 (MQ=255)
            gTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGcc                                                                                                                                      <  4:408308/150‑1 (MQ=255)
              cGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTg                                                                                                                                   <  3:182509/151‑1 (MQ=255)
               ggCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTgg                                                                                                                                  >  4:829100/1‑151 (MQ=255)
                gCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTgg                                                                                                                                  >  4:447025/1‑150 (MQ=255)
                 cATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGAt                                                                                                                                >  4:247563/1‑151 (MQ=255)
                  aTCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGAtt                                                                                                                               >  4:545158/1‑151 (MQ=255)
                  aTCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGAtt                                                                                                                               >  2:832879/1‑151 (MQ=255)
                    ccAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTgg                                                                                                                             <  1:259157/151‑1 (MQ=255)
                        ccTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGAc                                                                                                                         >  2:715186/1‑151 (MQ=255)
                           gctgGTGATTTGCCTTTCGCCAGTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTgg                                                                                                                      <  1:439105/151‑1 (MQ=255)
                           gctgGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTgg                                                                                                                      >  4:1127356/1‑151 (MQ=255)
                           gctgGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTg                                                                                                                       <  4:1490797/150‑1 (MQ=255)
                            ctgGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTgg                                                                                                                      <  2:1008986/150‑1 (MQ=255)
                             tgGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTggg                                                                                                                     >  4:864216/1‑150 (MQ=255)
                               gggATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGgtgt                                                                                                                  >  4:1186423/1‑151 (MQ=255)
                                 gATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTcg                                                                                                                >  3:368834/1‑151 (MQ=255)
                                 gATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTcg                                                                                                                >  4:851297/1‑151 (MQ=255)
                                  aTTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTcgc                                                                                                               <  3:454330/151‑1 (MQ=255)
                                            cACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATaaaaa                                                                                                     <  4:779925/151‑1 (MQ=255)
                                              ccATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAAt                                                                                                   <  4:1081521/151‑1 (MQ=255)
                                                aTTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATgg                                                                                                 >  2:996002/1‑151 (MQ=255)
                                                 atGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGTACATCTTTCTCGGCCGTGGATTGGTGAATGGGTGTCGCTCAATaaaa                                                                                                      >  1:27827/2‑145 (MQ=255)
                                                    cTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGAGACTGGGTGTCGCGCAATAAAAAATGGAtt                                                                                              <  1:70757/150‑1 (MQ=255)
                                                     tggttggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGc                                                                                            >  2:640400/1‑151 (MQ=255)
                                                      ggttggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGc                                                                                            <  2:1288427/150‑1 (MQ=255)
                                                      ggttggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCg                                                                                           >  4:903623/1‑151 (MQ=255)
                                                         tggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCg                                                                                           >  1:82558/1‑148 (MQ=255)
                                                         tggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGaa                                                                                         <  2:1119767/150‑1 (MQ=255)
                                                          ggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGaaa                                                                                        <  1:181287/150‑1 (MQ=255)
                                                          ggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGaaa                                                                                        >  2:1630545/1‑150 (MQ=255)
                                                          ggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGaaa                                                                                        >  2:922691/1‑150 (MQ=255)
                                                           gtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAAc                                                                                      <  4:276677/151‑1 (MQ=255)
                                                            tCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACt                                                                                     >  4:656860/1‑151 (MQ=255)
                                                            tCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACt                                                                                     >  1:90658/1‑151 (MQ=255)
                                                               ggTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACt                                                                                     <  4:1235795/148‑1 (MQ=255)
                                                               ggTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACt                                                                                     <  4:740338/148‑1 (MQ=255)
                                                                gTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGAcc                                                                                 <  3:45335/151‑1 (MQ=255)
                                                                  gatgaATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGAcc                                                                                 >  2:1538080/1‑149 (MQ=255)
                                                                  gatgaATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCa                                                                                <  3:246630/150‑1 (MQ=255)
                                                                     gaATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACg                                                                            >  4:415168/1‑151 (MQ=255)
                                                                         gTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTcc                                                                         >  2:716598/1‑150 (MQ=255)
                                                                           tCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCAtt                                                                      >  3:368578/1‑151 (MQ=255)
                                                                            cACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCAtt                                                                      <  4:718927/150‑1 (MQ=255)
                                                                            cACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTc                                                                     <  2:1201577/151‑1 (MQ=255)
                                                                             aCGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCt                                                                    <  1:398379/151‑1 (MQ=255)
                                                                                 tGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                >  1:379394/1‑151 (MQ=255)
                                                                                 tGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                >  3:361780/1‑151 (MQ=255)
                                                                                    aGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggtg                                                             >  2:1111301/1‑151 (MQ=255)
                                                                                    aGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggtg                                                             <  2:544402/151‑1 (MQ=255)
                                                                                    aGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggt                                                              <  3:303349/150‑1 (MQ=255)
                                                                                      ggggCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggtgg                                                            >  2:309790/1‑150 (MQ=255)
                                                                                        ggCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGttt                                                         <  4:1639917/151‑1 (MQ=255)
                                                                                             ccTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACa                                                    <  4:1446780/151‑1 (MQ=255)
                                                                                             ccTTGAGCAGATCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATAc                                                     >  4:782582/1‑150 (MQ=255)
                                                                                                    cAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATCCAGCGtt                                               >  2:1142736/1‑149 (MQ=255)
                                                                                                    cAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTc                                              >  2:1618621/1‑150 (MQ=255)
                                                                                                       gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCg                                          >  3:49971/1‑151 (MQ=255)
                                                                                                        tCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCg                                          >  2:430382/1‑150 (MQ=255)
                                                                                                         cGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGaa                                        <  2:1187663/151‑1 (MQ=255)
                                                                                                          ggTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAg                                       <  4:1053386/151‑1 (MQ=255)
                                                                                                            tAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGcc                                     >  1:186907/1‑151 (MQ=255)
                                                                                                            tAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGcc                                     >  2:1269306/1‑151 (MQ=255)
                                                                                                             aaaaTTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcg                                    >  3:289052/1‑151 (MQ=255)
                                                                                                               aaTTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgtc                                  <  4:1131125/151‑1 (MQ=255)
                                                                                                               aaTTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgtc                                  >  2:1159702/1‑151 (MQ=255)
                                                                                                                aTTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgtcg                                 >  4:1497725/1‑151 (MQ=255)
                                                                                                                  tATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGtt                               >  4:2009/1‑151 (MQ=255)
                                                                                                                     gctgcAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTaa                             <  2:689764/150‑1 (MQ=255)
                                                                                                                      ctgcAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATg                           >  3:202161/1‑151 (MQ=255)
                                                                                                                      ctgcAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATg                           >  2:389849/1‑151 (MQ=255)
                                                                                                                         cAACTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGt                         >  2:705388/1‑150 (MQ=255)
                                                                                                                          aaCTGCTGCTGCCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTAt                       >  2:407884/1‑151 (MQ=255)
                                                                                                                           aCTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATc                      >  3:19453/1‑151 (MQ=255)
                                                                                                                              gctgctgcCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCt                     <  1:198719/149‑1 (MQ=255)
                                                                                                                              gctgctgcCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTgg                   <  3:276925/151‑1 (MQ=255)
                                                                                                                              gctgctgcCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTg                    <  2:1513688/150‑1 (MQ=255)
                                                                                                                              gctgctgcCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTg                    <  3:187838/150‑1 (MQ=255)
                                                                                                                                tgctgcCGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGc                  >  4:116248/1‑150 (MQ=255)
                                                                                                                                     ccGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATaa              >  2:764553/1‑149 (MQ=255)
                                                                                                                                     ccGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAg            <  4:944329/151‑1 (MQ=255)
                                                                                                                                      cGTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGt           >  2:727806/1‑151 (MQ=255)
                                                                                                                                       gTTTGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGtt          >  2:943618/1‑151 (MQ=255)
                                                                                                                                         ttGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTgg        >  4:1297418/1‑151 (MQ=255)
                                                                                                                                         ttGTGTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTg         <  2:1313743/150‑1 (MQ=255)
                                                                                                                                          tgtgTTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTgg        <  4:833979/150‑1 (MQ=255)
                                                                                                                                           gtgtTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCt      <  3:192637/151‑1 (MQ=255)
                                                                                                                                             gtTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTg                    >  3:274464/1‑135 (MQ=255)
                                                                                                                                             gtTGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTg                    <  1:274448/135‑1 (MQ=255)
                                                                                                                                               tGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTgggg  >  1:446006/1‑151 (MQ=255)
                                                                                                                                               tGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTgggg  >  3:86466/1‑151 (MQ=255)
                                                                                                                                               tGGGACATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTggg   <  4:716145/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTTTGTTCTGCGTCGGCATCCAGCCTGCTGGGGATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGG  >  NC_000913/2527332‑2527625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A