Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I2 R1
|
193 |
39.9 |
1341687 |
91.8% |
1231668 |
149.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
650,431 |
C→T |
V20V (GTG→GTA) |
citE ← |
citrate lyase, citryl‑ACP lyase (beta) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 650,431 | 0 | C | T | 96.9%
| 106.5
/ ‑5.5
| 32 | V20V (GTG→GTA) | citE | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (18/13); total (18/14) |
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
GATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCA > NC_000913/650286‑650572
|
gATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTacaaa < 1:443737/151‑1 (MQ=255)
cAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAaacaac < 2:274921/151‑1 (MQ=255)
gTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTgcg > 2:324691/1‑151 (MQ=255)
ttGCAGCGCGTGGTAAACCATGCGGCGGGCGGGGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTgcgg > 2:98610/1‑151 (MQ=255)
ccAGCGCGTGGTAAACCATGCGGCGGGCGGGGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATAAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTgcggc‑g > 1:650526/2‑151 (MQ=255)
aGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑ggg > 1:556653/1‑151 (MQ=255)
cgcgTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGGCAAACATCAGGGCAACAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCCCCAGGTACAAACAACATGCTGCGGC‑ggg > 1:479127/1‑149 (MQ=255)
gcgTGGTAAACCATGCGTCGGTCTGTGTCTTTTTCACGCAATGATCCTGAGTCTCCGAGGTCAAACATCCGCGCATCCGTCGGGTCGTTGAAGGAGTTGCTTCCCTTCTCCGCCTTGGCTCCACGCATTAAAAACATGCTGGGGG‑GGGTgg < 1:285776/151‑2 (MQ=255)
aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACgtt < 1:98608/151‑1 (MQ=255)
gcggcgGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACgttgt < 2:378994/151‑1 (MQ=255)
ggcggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGc < 2:416535/151‑1 (MQ=255)
cggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGa < 2:398332/151‑1 (MQ=255)
tCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGTTTTTTGTTGTTGCAGCGAAGCgg > 1:66974/1‑151 (MQ=255)
tttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGGTTTACGTTGTTggagcggagccg > 1:60220/1‑147 (MQ=255)
tCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGGGGGGTTTTCGTTGTTGCAGCGAAGAGG‑gaa > 2:502713/1‑148 (MQ=255)
aCGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGGGTTTTTCGTTGTTTCAGCGAAGCGG‑GAATCAtt > 2:395742/1‑151 (MQ=255)
aCGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCAtt < 1:354114/151‑1 (MQ=255)
aaTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGGTTTACGTTGTTGGAGAGAAGCGG‑AAATCAtttg > 2:386759/1‑147 (MQ=255)
aaTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAAACATTGGc > 2:294184/1‑150 (MQ=255)
cTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGGGGTTTTTGTTGTTGCAGGGAAGAGG‑GAAACATTTGCAAtcc > 1:67779/1‑151 (MQ=255)
ggAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGAGGTTTTCGTTGTTGCAGCGGAGCGG‑AAATAATTTGCAATCCTccc > 2:461242/1‑151 (MQ=255)
tCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGAGTTTTTCGTTGTTTCAGCGAAACGG‑GAATCATTGGCAATCCCCCCCTgg > 2:183219/1‑151 (MQ=255)
tCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGc < 2:614648/149‑1 (MQ=255)
aGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGCGGGGGGGGGGTTTTAGGTT‑TTGGAGGGAAGAGGAAAATCTTTGGAATTcccccctcg > 1:257779/1‑142 (MQ=255)
ggTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGcc < 1:324689/151‑1 (MQ=255)
aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGGTTTTTGTTGTTTCAGCGAAGCGG‑GAAACATTTGCAATCCTCCCCCGGCAGAGCCggg > 1:614844/1‑149 (MQ=255)
cATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGGGGGGTTTTCGTTGTTGCCGCGAAGCGG‑AAATCATTTGCCATCCTCCCATGGCAGAGCCggg > 1:485157/1‑147 (MQ=255)
cATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGgcg < 2:375944/151‑1 (MQ=255)
cAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGt < 1:396773/151‑1 (MQ=255)
aCCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGGGGGGTTTTCGTTGTTGTAGTGGAGCGG‑GAAACATTGGGAATCCTCCCCTGGCAGAGACGGGGGAACCCCGGGGCGTGGCAACCGGGGTTccccgcgtg > 1:515614/1‑140 (MQ=255)
cgcgGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTaaaa < 2:521447/151‑1 (MQ=255)
ggCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCa < 1:139959/151‑1 (MQ=255)
|
GATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCA > NC_000913/650286‑650572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
