Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	693,599	T→C	T453A (ACA→GCA)	miaB ←	tRNA‑i(6)A37 methylthiotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	693,599	0	T	C	94.4%	116.7 / ‑1.2	36	T453A (ACA→GCA)	miaB	tRNA‑i(6)A37 methylthiotransferase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (17/17); total (17/19)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

AAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCG  >  NC_000913/693453‑693745

aaaaaGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGGCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCt                                                                                                                                                >  1:592371/1‑151 (MQ=255)

   aaGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGcc                                                                                                                                             >  2:359253/1‑151 (MQ=255)

          aaGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCa                                                                                                                                      <  1:533419/151‑1 (MQ=255)

           aGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAg                                                                                                                                     <  2:331709/151‑1 (MQ=255)

              aTTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGAcc                                                                                                                                  >  2:363941/1‑151 (MQ=255)

                tGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGAccc                                                                                                                                 >  1:65086/1‑150 (MQ=255)

                      aaaGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCa                                                                                                                           <  2:40819/150‑1 (MQ=255)

                      aaaGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCAt                                                                                                                          <  1:411284/151‑1 (MQ=255)

                           tGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTc                                                                                                                      >  1:464344/1‑150 (MQ=255)

                               gATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTa                                                                                                                  >  2:447643/1‑150 (MQ=255)

                               gATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTAc                                                                                                                 <  1:447638/151‑1 (MQ=255)

                                       gagaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACt                                                                                                         >  2:67908/1‑151 (MQ=255)

                                          aaaaaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTa                                                                                                      <  2:485076/151‑1 (MQ=255)

                                           aaaaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTAc                                                                                                     <  1:205638/151‑1 (MQ=255)

                                             aaaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACcg                                                                                                   <  1:495431/151‑1 (MQ=255)

                                              aaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACcgc                                                                                                  >  2:613185/1‑151 (MQ=255)

                                                     cccACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCagaga                                                                                           <  2:436037/151‑1 (MQ=255)

                                                     cccACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATGTCATCTTCAGTACGCACCACTTTACCGCGCagaga                                                                                           <  1:512577/151‑1 (MQ=255)

                                                           ggAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCgg                                                                                     >  2:385409/1‑151 (MQ=255)

                                                              aCGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTa                                                                                  <  1:169853/151‑1 (MQ=255)

                                                                   aGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGt                                                                             <  1:145009/151‑1 (MQ=255)

                                                                    ggCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTc                                                                            <  2:609315/151‑1 (MQ=255)

                                                                      ccTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCgg                                                                          >  1:622737/1‑151 (MQ=255)

                                                                         gagaATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCGCTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGt                                                                         <  2:434971/149‑1 (MQ=255)

                                                                          agaATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAAt                                                                      <  2:12130/151‑1 (MQ=255)

                                                                                    cTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcg                                                            >  1:600840/1‑151 (MQ=255)

                                                                                      gATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCATCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcg                                                            <  1:74619/149‑1 (MQ=255)

                                                                                                 aCGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCg                                                >  1:260900/1‑150 (MQ=255)

                                                                                                      aaGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCat                                           >  2:444979/1‑150 (MQ=255)

                                                                                                       aGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCatat                                         <  2:332147/151‑1 (MQ=255)

                                                                                                          tCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCg                                      >  2:661279/1‑151 (MQ=255)

                                                                                                            gTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGc                                    <  1:599990/151‑1 (MQ=255)

                                                                                                                 cTTTGCGGGTACGGGCAATCACTGATTCCGGTGCTTCTGCCCCCCGCAGCCCAATTTCACCTTCAGAACGAACACCTTTACCGCGCAGAGGTTTCGGGTAGCCGCCGGTATTTTCTCCATCAACGATTTTACCGACCATATccgccggcc                                >  1:212468/1‑147 (MQ=255)

                                                                                                                         gTACGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGt                       <  1:291175/151‑1 (MQ=255)

                                                                                                                            cGGGCAATCACTGATTCCGGTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTg                     >  1:621638/1‑150 (MQ=255)

                                                                                                                                              ggTGCTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCg  >  2:495167/1‑151 (MQ=255)

AAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCG  >  NC_000913/693453‑693745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A