Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I1 R1 170 39.5 1266514 99.2% 1256381 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 694,122 G→A G278G (GGC→GGT)  miaB ← tRNA‑i(6)A37 methylthiotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913694,1220GA100.0% 161.1 / NA 41G278G (GGC→GGT) miaBtRNA‑i(6)A37 methylthiotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/28);  total (13/28)

CAATCAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGGCCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATACGATCGATCC  >  NC_000913/693980‑694267
                                                                                                                                              |                                                                                                                                                 
cAATCAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAg                                                                                                                                           <  1:306770/151‑1 (MQ=255)
    cAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTc                                                                                                                                       <  2:433166/151‑1 (MQ=255)
     aGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTc                                                                                                                                       >  2:582937/1‑150 (MQ=255)
         tCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGaa                                                                                                                                   <  1:60825/150‑1 (MQ=255)
          cATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATg                                                                                                                                 <  2:382325/151‑1 (MQ=255)
               ttttCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATc                                                                                                                            >  1:306038/1‑151 (MQ=255)
                tttCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATc                                                                                                                            <  1:212067/150‑1 (MQ=255)
                  tctcGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCgg                                                                                                                          >  2:546004/1‑150 (MQ=255)
                    tcGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAAc                                                                                                                       >  1:35171/1‑151 (MQ=255)
                     cGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGa                                                                                                                         <  1:291963/148‑1 (MQ=255)
                                  ggtTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCgg                                                                                                          <  1:316611/150‑1 (MQ=255)
                                      tCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGc                                                                                                         >  1:344385/1‑147 (MQ=255)
                                        gCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGc                                                                                                   >  2:259480/1‑151 (MQ=255)
                                          cAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAg                                                                                                 <  1:326685/151‑1 (MQ=255)
                                              gAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGa                                                                                               >  1:229807/1‑149 (MQ=255)
                                                     aCGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCAcc                                                                                       >  1:18344/1‑150 (MQ=255)
                                                     aCGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCa                                                                                      <  1:199224/151‑1 (MQ=255)
                                                            aaTCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCgg                                                                               >  1:29633/1‑151 (MQ=255)
                                                                   aCTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTcg                                                                        <  1:380050/151‑1 (MQ=255)
                                                                   aCTGATCTGAATACCTGGCCCCGCCGCACTCTGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAAATGCAGGTAGCCGACCAGCTCCGGCGTGTcg                                                                        <  2:213564/151‑1 (MQ=255)
                                                                      gATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTcgc                                                                       <  1:293576/149‑1 (MQ=255)
                                                                         cTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATac                                                                  >  2:187776/1‑151 (MQ=255)
                                                                            aaTATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACt                                                               <  2:248306/151‑1 (MQ=255)
                                                                                      cgcgCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGata                                                      <  2:352037/150‑1 (MQ=255)
                                                                                       gcgcCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATAtc                                                    <  1:377705/151‑1 (MQ=255)
                                                                                            gcacgcaGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATAtcgtcg                                                <  2:237828/150‑1 (MQ=255)
                                                                                              acgcaGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGatat                                                     <  2:87816/143‑1 (MQ=255)
                                                                                              acgcaGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGatat                                                     >  1:87816/1‑143 (MQ=255)
                                                                                                 caGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCACATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAAt                                          >  1:225134/1‑151 (MQ=255)
                                                                                                        cGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCTGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCg                                   <  2:422429/151‑1 (MQ=255)
                                                                                                         ggATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCgg                                  >  2:594271/1‑151 (MQ=255)
                                                                                                           atgatCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGAt                                <  1:480907/151‑1 (MQ=255)
                                                                                                                 gCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCtgg                          <  1:360976/151‑1 (MQ=255)
                                                                                                                       tACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTa                     <  1:101345/150‑1 (MQ=255)
                                                                                                                        aCTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTaaa                   <  2:470925/151‑1 (MQ=255)
                                                                                                                        aCTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTaa                    <  2:182337/150‑1 (MQ=255)
                                                                                                                           ccAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAg                  <  1:476813/149‑1 (MQ=255)
                                                                                                                            cAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCACATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGa               <  2:225129/151‑1 (MQ=255)
                                                                                                                              gcgcCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAAt             <  1:134655/151‑1 (MQ=255)
                                                                                                                                     aTGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATAcgatc       <  2:541634/150‑1 (MQ=255)
                                                                                                                                         gTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATACGATCGATcc  <  2:306031/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CAATCAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGGCCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATACGATCGATCC  >  NC_000913/693980‑694267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A