Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	717,098	A→G	F390L (TTT→CTT)	potE ←	putrescine/proton symporter: putrescine/ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	717,098	0	A	G	100.0%	100.7 / NA	28	F390L (TTT→CTT)	potE	putrescine/proton symporter: putrescine/ornithine antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/13); total (15/13)

CGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAG  >  NC_000913/716951‑717233

cGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGtt                                                                                                                                      <  2:651148/151‑1 (MQ=255)

   gtTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGc                                                                                                                                   <  2:206043/151‑1 (MQ=255)

   gtTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGc                                                                                                                                   <  2:104716/151‑1 (MQ=255)

     ttATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCa                                                                                                                                  >  1:413069/1‑150 (MQ=255)

           ttCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCg                                                                                                                           >  2:137999/1‑151 (MQ=255)

            tCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCg                                                                                                                           <  1:43034/150‑1 (MQ=255)

              aGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCtt                                                                                                                        >  2:48955/1‑151 (MQ=255)

                   aaaGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTg                                                                                                                     >  1:196853/1‑149 (MQ=255)

                          ggtgATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGca                                                                                                            <  1:113043/151‑1 (MQ=255)

                             gATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGcaca                                                                                                          <  1:323056/150‑1 (MQ=255)

                                     aCCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGTGCACATTAGCGAcc                                                                                                 <  1:515409/151‑1 (MQ=255)

                                      ccATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCt                                                                                                >  2:276687/1‑151 (MQ=255)

                                                    accaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACt                                                                                  >  2:263088/1‑151 (MQ=255)

                                                            aggTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATgccgc                                                                          <  2:518958/151‑1 (MQ=255)

                                                                 aCGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTg                                                                     <  1:137646/151‑1 (MQ=255)

                                                                  cGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGa                                                                    <  1:601069/151‑1 (MQ=255)

                                                                               cAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACgg                                                        >  2:33612/1‑150 (MQ=255)

                                                                                aGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGaa                                                      <  1:665743/151‑1 (MQ=255)

                                                                                  cATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATg                                                    >  2:347846/1‑151 (MQ=255)

                                                                                          cttcCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGt                                            >  2:76011/1‑151 (MQ=255)

                                                                                              cccGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTcacc                                        >  2:382797/1‑151 (MQ=255)

                                                                                                     gAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCa                                 <  2:56092/151‑1 (MQ=255)

                                                                                                             cgcATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAAcc                         >  1:616076/1‑151 (MQ=255)

                                                                                                                    aaGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGt                  >  2:475611/1‑151 (MQ=255)

                                                                                                                          taCATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAact            >  2:661450/1‑151 (MQ=255)

                                                                                                                                  gcCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGttt    >  1:101506/1‑151 (MQ=255)

                                                                                                                                   ccAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGttt    >  2:352549/1‑150 (MQ=255)

                                                                                                                                    caacaaAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAg  <  2:482971/151‑1 (MQ=255)

CGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAG  >  NC_000913/716951‑717233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A