Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	847,016	A→G	V35A (GTA→GCA)	glnP ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	847,016	0	A	G	100.0%	131.2 / NA	36	V35A (GTA→GCA)	glnP	glutamine transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/22); total (14/22)

CGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATC  >  NC_000913/846870‑847151

cGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGcc                                                                                                                                       >  2:313623/1‑149 (MQ=255)

cGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCa                                                                                                                                      <  2:334045/150‑1 (MQ=255)

                 aGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGa                                                                                                                    <  2:594969/151‑1 (MQ=255)

                   ggCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAgg                                                                                                                  <  1:372252/151‑1 (MQ=255)

                       aaataaatAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCg                                                                                                              <  2:234443/151‑1 (MQ=255)

                               aaaCATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCcac                                                                                                      <  1:322931/151‑1 (MQ=255)

                                aaCATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCcac                                                                                                      >  2:339411/1‑150 (MQ=255)

                                 aCATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAg                                                                                                    <  1:20931/151‑1 (MQ=255)

                                        cTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCAtt                                                                                             >  2:75600/1‑151 (MQ=255)

                                           gacgacGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTg                                                                                          <  1:323841/151‑1 (MQ=255)

                                                 gaTAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCt                                                                                    <  1:371384/151‑1 (MQ=255)

                                                  aTAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCtt                                                                                   >  2:512493/1‑151 (MQ=255)

                                                  aTAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCtt                                                                                   >  1:379351/1‑151 (MQ=255)

                                                    aGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCa                                                                                 <  1:136905/151‑1 (MQ=255)

                                                     ggagtgCCGCGGATCACTTCAATAAAGACCAGCGCGGCGTGGTTGGCTATCCAACCTCCGAAGCAGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCa                                                                                 >  1:603510/4‑150 (MQ=255)

                                                     ggTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCaa                                                                                <  2:577281/151‑1 (MQ=255)

                                                      gtgtGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAAt                                                                               >  2:266969/1‑151 (MQ=255)

                                                           ccGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGaa                                                                          <  2:569705/151‑1 (MQ=255)

                                                             gcgGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGc                                                                        >  1:332213/1‑151 (MQ=255)

                                                                 aTCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGaa                                                                    <  2:293650/151‑1 (MQ=255)

                                                                 aTCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGa                                                                     >  1:209207/1‑150 (MQ=255)

                                                                   cACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATg                                                                  <  1:100950/151‑1 (MQ=255)

                                                                           tAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGcc                                                           >  1:453918/1‑150 (MQ=255)

                                                                                  cAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGc                                                    >  2:217660/1‑150 (MQ=255)

                                                                                     cgcgACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTc                                                <  2:552466/151‑1 (MQ=255)

                                                                                           gTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCa                                          <  1:530030/151‑1 (MQ=255)

                                                                                                 ggCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGc                                    >  2:236939/1‑151 (MQ=255)

                                                                                                                 aaGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGtt                    >  1:575044/1‑151 (MQ=255)

                                                                                                                  aGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTa                   <  1:333623/151‑1 (MQ=255)

                                                                                                                  aGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTa                   <  1:354328/151‑1 (MQ=255)

                                                                                                                      gcgtgcAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCgt               <  1:330866/151‑1 (MQ=255)

                                                                                                                      gcgtgcAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCgt               <  1:512490/151‑1 (MQ=255)

                                                                                                                           cAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGgtgt          <  2:561905/151‑1 (MQ=255)

                                                                                                                              aaCCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTaaa       <  2:209208/151‑1 (MQ=255)

                                                                                                                                  tGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAaataat   >  1:236972/1‑151 (MQ=255)

                                                                                                                                   gCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATc  <  2:453923/151‑1 (MQ=255)

CGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATC  >  NC_000913/846870‑847151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A