Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	870,807	C→T	A466V (GCG→GTG)	gsiB →	glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	870,807	0	C	T	97.4%	127.7 / ‑4.2	38	A466V (GCG→GTG)	gsiB	glutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (22/15); total (22/16)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GGGCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAAC  >  NC_000913/870663‑870954

gggCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGgcgc                                                                                                                                                >  2:324304/1‑150 (MQ=255)

 ggCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCCCCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAg                                                                                                                                              <  1:70817/151‑1 (MQ=255)

  gCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAgg                                                                                                                                             <  1:390321/151‑1 (MQ=255)

    aCTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGat                                                                                                                                           >  1:669901/1‑151 (MQ=255)

         cgccgcTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATatcat                                                                                                                                      <  2:619370/151‑1 (MQ=255)

         cgccgcTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATatca                                                                                                                                       <  1:374264/150‑1 (MQ=255)

            cgcTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTg                                                                                                                                   <  1:340206/151‑1 (MQ=255)

                   tGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGa                                                                                                                             >  2:385782/1‑150 (MQ=255)

                                tGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATcc                                                                                                               >  1:630926/1‑151 (MQ=255)

                                             tGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTggtggt                                                                                                     >  1:556656/1‑148 (MQ=255)

                                                  aaTACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAAc                                                                                             >  2:598102/1‑151 (MQ=255)

                                                           ttttACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCgg                                                                                    >  1:208889/1‑151 (MQ=255)

                                                           ttttACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCgg                                                                                    >  2:437981/1‑151 (MQ=255)

                                                              tACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGca                                                                                  <  1:9846/150‑1 (MQ=255)

                                                                        aaCAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTaaaa                                                                        >  2:221598/1‑150 (MQ=255)

                                                                           aGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCt                                                                    <  2:669821/151‑1 (MQ=255)

                                                                               ggATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGAcc                                                                >  2:499481/1‑151 (MQ=255)

                                                                                    aCTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGttt                                                           >  1:151910/1‑151 (MQ=255)

                                                                                      ttCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGttttt                                                         >  1:657427/1‑151 (MQ=255)

                                                                                      ttCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGttttt                                                         >  2:518453/1‑151 (MQ=255)

                                                                                       tCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGttttt                                                         <  1:536572/150‑1 (MQ=255)

                                                                                        ccTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTgg                                                       <  2:198536/151‑1 (MQ=255)

                                                                                               cAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGc                                                >  2:480350/1‑151 (MQ=255)

                                                                                               cAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCAt                                                  <  2:530861/149‑1 (MQ=255)

                                                                                                aGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGcc                                               >  2:177834/1‑151 (MQ=255)

                                                                                                  gCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGcc                                               >  2:328371/1‑149 (MQ=255)

                                                                                                         aaaCTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCgg                                      >  2:146463/1‑151 (MQ=255)

                                                                                                           aCTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCt                                    >  1:120816/1‑151 (MQ=255)

                                                                                                                    ccGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACGCCGGCTTCAGCttt                            <  1:635797/150‑1 (MQ=255)

                                                                                                                    ccGGCGGAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCttt                            <  2:444248/150‑1 (MQ=255)

                                                                                                                      ggcggAAAAGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGa                          >  2:567117/1‑150 (MQ=255)

                                                                                                                              aGACCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCgg                  >  2:510009/1‑150 (MQ=255)

                                                                                                                                aCCCGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGAtt               >  1:54678/1‑151 (MQ=255)

                                                                                                                                 cccGCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGAttt              <  1:71196/151‑1 (MQ=255)

                                                                                                                                    gCTTATATAAGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCTGCTTCAGCTTTGAAGACGCGGATTTGCa           <  1:163296/151‑1 (MQ=255)

                                                                                                                                            aaGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCaa   <  1:408530/151‑1 (MQ=255)

                                                                                                                                            aaGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCCGATTTGCAATa        >  1:289298/1‑146 (MQ=255)

                                                                                                                                             aGGTGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAAc  <  2:128752/151‑1 (MQ=255)

GGGCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAAC  >  NC_000913/870663‑870954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A