Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 913,520 Δ1 bp coding (295/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913913,5180T.100.0% 206.5 / NA 43coding (297/1653 nt)hcphybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/25);  total (18/25)

GAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGCTTTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCT  >  NC_000913/913368‑913662
                                                                                                                                                      |                                                                                                                                                
gagaATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGCt                                                                                                                                                  >  2:142637/1‑151 (MQ=255)
            gCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCCCCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGCttcag                                                                                                                                              <  2:540285/143‑4 (MQ=255)
            gCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTcgc                                                                                                                                     >  2:143692/1‑151 (MQ=255)
                  cgccgcTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAggg                                                                                                                               >  2:605145/1‑151 (MQ=255)
                              tttGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCAc                                                                                                                   >  1:44022/1‑151 (MQ=255)
                                tGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACg                                                                                                                  >  1:570362/1‑150 (MQ=255)
                                     ggggTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTa                                                                                                            <  2:503706/151‑1 (MQ=255)
                                                tgctgcCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATAc                                                                                                 >  2:127293/1‑151 (MQ=255)
                                                        gACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGgaga                                                                                          <  2:603703/150‑1 (MQ=255)
                                                        gACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGgaga                                                                                          <  1:229169/150‑1 (MQ=255)
                                                               cAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCg                                                                                      >  2:657090/1‑147 (MQ=255)
                                                               cAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCg                                                                                      <  1:657079/147‑1 (MQ=255)
                                                                     gCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGt                                                                            <  2:75099/151‑1 (MQ=255)
                                                                      ccGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGtt                                                                           <  2:403043/151‑1 (MQ=255)
                                                                       cGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTg                                                                          <  1:569061/151‑1 (MQ=255)
                                                                       cGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTg                                                                          <  1:576290/151‑1 (MQ=255)
                                                                        gagaTCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTgg                                                                         >  1:184515/1‑151 (MQ=255)
                                                                        gagaTCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTgg                                                                         <  1:157225/151‑1 (MQ=255)
                                                                         agagCGTCGCCTACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCTTTTGCAGCTACAGCCAGGCATTGCGC‑TTCAGCGCATCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTg                                                                          <  1:611672/149‑1 (MQ=255)
                                                                             cgtcgCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAg                                                                     <  1:49724/150‑1 (MQ=255)
                                                                              gtcgCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAggg                                                                   >  2:343560/1‑151 (MQ=255)
                                                                                    cACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTga                                                               <  1:662504/149‑1 (MQ=255)
                                                                                    cACCAGTGGCAGGTCAGCCATCGGGTTATCGTCGCGCGCGTTGGCAGCTACAGCCTGGCATTGCGC‑TTCATCGCCTCGCGCAGAGCACTCTCTCCACGAGCGTAGCCGACAAGACGCGGAGAATCGAAGTTAACGTTGGTCAGTGTTgaga                                                             <  1:332722/151‑1 (MQ=255)
                                                                                       cAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGaaa                                                           >  1:237918/1‑150 (MQ=255)
                                                                                            gCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCa                                                     <  2:531907/151‑1 (MQ=255)
                                                                                               ggTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGt                                                  <  1:127290/151‑1 (MQ=255)
                                                                                                gTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTg                                                 >  2:104034/1‑151 (MQ=255)
                                                                                                       aTCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGaa                                          <  1:605139/151‑1 (MQ=255)
                                                                                                          gggTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCt                                       >  1:91598/1‑151 (MQ=255)
                                                                                                           ggTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTg                                      <  1:293089/151‑1 (MQ=255)
                                                                                                             ttATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTc                                    >  2:171808/1‑151 (MQ=255)
                                                                                                              tATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTc                                    <  2:300085/150‑1 (MQ=255)
                                                                                                              tATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTc                                    >  1:131076/1‑150 (MQ=255)
                                                                                                                  gACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACAt                               >  2:267640/1‑151 (MQ=255)
                                                                                                                      cgcgcgTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTg                           <  1:104031/151‑1 (MQ=255)
                                                                                                                       gcgcgTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTgg                          >  2:517073/1‑151 (MQ=255)
                                                                                                                          cgTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTtg                       <  1:648369/151‑1 (MQ=255)
                                                                                                                          cgTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTtg                       <  2:97307/151‑1 (MQ=255)
                                                                                                                           gTTGGCATCTACAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTtg                       >  2:23625/1‑150 (MQ=255)
                                                                                                                                     aCAGCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGcc               <  1:143688/148‑1 (MQ=255)
                                                                                                                                        gCCAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTc         <  1:62538/151‑1 (MQ=255)
                                                                                                                                         ccAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTc         >  1:207340/1‑150 (MQ=255)
                                                                                                                                          cAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCAc       <  1:707/151‑1 (MQ=255)
                                                                                                                                          cAGGCATTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCAc       >  1:531109/1‑151 (MQ=255)
                                                                                                                                               aTTGCGC‑TTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCt  <  1:171806/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACGCGCGCGTTGGCATCTACAGCCAGGCATTGCGCTTTCAGCGCCTCGCGCAGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCT  >  NC_000913/913368‑913662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A