Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,314,744	T→C	T161A (ACT→GCT)	yciE ←	putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,314,744	0	T	C	100.0%	147.2 / NA	39	T161A (ACT→GCT)	yciE	putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/16); total (23/16)

AACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTG  >  NC_000913/1314597‑1314887

aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTc                                                                                                                                              >  1:663347/1‑151 (MQ=255)

     gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGc                                                                                                                                         >  2:47047/1‑151 (MQ=255)

         gCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAAt                                                                                                                                     <  1:627782/151‑1 (MQ=255)

             aCGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTaa                                                                                                                                  <  1:5280/150‑1 (MQ=255)

                     aTCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTtctc                                                                                                                         <  1:194933/151‑1 (MQ=255)

                         gTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAgtt                                                                                                                     >  2:276024/1‑151 (MQ=255)

                            ggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAgttgtt                                                                                                                  >  2:626995/1‑151 (MQ=255)

                                          cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTg                                                                                                    <  2:295207/151‑1 (MQ=255)

                                               ggATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATc                                                                                                >  2:561471/1‑150 (MQ=255)

                                                 atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGc                                                                                             <  1:275374/151‑1 (MQ=255)

                                                  tatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                                                                            >  1:140536/1‑151 (MQ=255)

                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                                                                            >  1:307077/1‑150 (MQ=255)

                                                     cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAg                                                                                          <  1:3321/150‑1 (MQ=255)

                                                      gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                                                                        >  2:99151/1‑151 (MQ=255)

                                                      gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                                                                        <  1:623376/151‑1 (MQ=255)

                                                          ttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGcc                                                                                    >  1:43828/1‑151 (MQ=255)

                                                          ttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGc                                                                                     <  2:486606/150‑1 (MQ=255)

                                                            aaTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCa                                                                                   <  1:171770/150‑1 (MQ=255)

                                                                ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGTCCATTTg                                                                               <  1:488680/150‑1 (MQ=255)

                                                                 cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                                                                             >  1:41856/1‑151 (MQ=255)

                                                                              ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaa                                                                <  2:113479/151‑1 (MQ=255)

                                                                               cccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa                                                               <  1:561466/151‑1 (MQ=255)

                                                                                     atatACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCt                                                         <  2:43827/151‑1 (MQ=255)

                                                                                      tataCGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCtt                                                        >  2:29819/1‑151 (MQ=255)

                                                                                          cGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGAt                                                    >  1:450410/1‑151 (MQ=255)

                                                                                               tCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTg                                               >  2:358742/1‑151 (MQ=255)

                                                                                                     aGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTg                                         >  1:407786/1‑151 (MQ=255)

                                                                                                       gACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGaa                                       <  2:550247/151‑1 (MQ=255)

                                                                                                           tGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTg                                   >  1:547535/1‑151 (MQ=255)

                                                                                                              tataAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTAt                                >  2:228976/1‑151 (MQ=255)

                                                                                                              tataAAGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTAt                                >  2:94606/1‑151 (MQ=255)

                                                                                                                  aaGGGTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCAcc                            >  1:590026/1‑151 (MQ=255)

                                                                                                                      gTATTATTTCTTCGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGc                         >  2:284814/1‑150 (MQ=255)

                                                                                                                                 tcGCTTCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTtgctgc             >  2:232425/1‑151 (MQ=255)

                                                                                                                                      tCTACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTtgctgctgc          <  1:94604/149‑1 (MQ=255)

                                                                                                                                        tACGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAt      >  2:610370/1‑151 (MQ=255)

                                                                                                                                         aCGCCATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAAt      <  1:590177/150‑1 (MQ=255)

                                                                                                                                             cATCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTg  >  2:412179/1‑150 (MQ=255)

                                                                                                                                              aTCAGCTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTg  >  2:249404/1‑149 (MQ=255)

AACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTG  >  NC_000913/1314597‑1314887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A