Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,045	T→C	Q288Q (CAA→CAG)	puuA ←	glutamate‑‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,045	0	T	C	100.0%	146.9 / NA	38	Q288Q (CAA→CAG)	puuA	glutamate‑‑putrescine ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/17); total (21/17)

CGATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACG  >  NC_000913/1359911‑1360189

cgATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTgatatgga                                                                                                                                  <  1:227483/151‑1 (MQ=255)

    aCGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTgatatggatatg                                                                                                                              >  2:159216/1‑151 (MQ=255)

          tCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTc                                                                                                                         <  1:26096/150‑1 (MQ=255)

              gTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTg                                                                                                                    <  2:633221/151‑1 (MQ=255)

                      ccAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTg                                                                                                            >  1:326287/1‑151 (MQ=255)

                            aCGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTc                                                                                                      >  1:117395/1‑151 (MQ=255)

                             cGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTc                                                                                                      >  1:652836/1‑150 (MQ=255)

                               ccATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATa                                                                                                   <  2:496968/151‑1 (MQ=255)

                               ccATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATa                                                                                                   <  1:654600/151‑1 (MQ=255)

                                         cgGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGcc                                                                                         <  2:326289/151‑1 (MQ=255)

                                                  gTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTg                                                                                >  2:255779/1‑151 (MQ=255)

                                                         atcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGc                                                                          >  1:16848/1‑150 (MQ=255)

                                                              ggCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTa                                                                    <  1:44276/151‑1 (MQ=255)

                                                              ggCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTa                                                                    <  1:569811/151‑1 (MQ=255)

                                                                cGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATg                                                                  >  2:269184/1‑151 (MQ=255)

                                                                   gCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCtt                                                               <  1:201907/151‑1 (MQ=255)

                                                                     aTCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCtttt                                                             >  2:196274/1‑151 (MQ=255)

                                                                              agcagcGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATcg                                                    >  1:448790/1‑150 (MQ=255)

                                                                                     gcgAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCAc                                             <  2:203294/151‑1 (MQ=255)

                                                                                       gAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCAcc                                            >  2:393648/1‑150 (MQ=255)

                                                                                       gAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCa                                           <  1:139327/151‑1 (MQ=255)

                                                                                          tCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAgg                                         >  1:168368/1‑150 (MQ=255)

                                                                                             tCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAgg                                         <  1:52227/147‑1 (MQ=255)

                                                                                             tCGCCTTCCGCGTCAGAAAGCACGTTCTAGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGATCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGtt                                     >  1:446414/1‑151 (MQ=255)

                                                                                              cGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGttt                                    <  2:618699/151‑1 (MQ=255)

                                                                                                  ttCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGt                                >  1:156282/1‑151 (MQ=255)

                                                                                                   tCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTg                               >  1:210455/1‑151 (MQ=255)

                                                                                                    ccGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCCTCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGc                              >  2:335610/1‑151 (MQ=255)

                                                                                                           aGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGca                       <  1:337399/151‑1 (MQ=255)

                                                                                                             aaaGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGcatc                     >  2:71792/1‑151 (MQ=255)

                                                                                                                gCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCatcatc                  <  1:535721/151‑1 (MQ=255)

                                                                                                                gCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCatcatc                  >  2:659805/1‑151 (MQ=255)

                                                                                                                    gTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGc                >  1:386757/1‑149 (MQ=255)

                                                                                                                          gCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTcc        <  2:515453/151‑1 (MQ=255)

                                                                                                                            cACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTcc        >  1:652983/1‑149 (MQ=255)

                                                                                                                               gATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCAc   >  1:174521/1‑151 (MQ=255)

                                                                                                                                attattCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACg  <  2:39048/151‑1 (MQ=255)

                                                                                                                                 ttattCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACg  >  1:231871/1‑150 (MQ=255)

CGATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACG  >  NC_000913/1359911‑1360189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A