Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,363,578 A→G D279G (GAC→GGC)  puuC → gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,363,5780AG100.0% 176.9 / NA 52D279G (GAC→GGC) puuCgamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

GCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGC  >  NC_000913/1363432‑1363721
                                                                                                                                                  |                                                                                                                                               
gcTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGc                                                                                                                                             >  1:540781/1‑151 (MQ=255)
  tGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGccc                                                                                                                                           >  1:518817/1‑151 (MQ=255)
      gcgTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGAt                                                                                                                                       >  2:587945/1‑151 (MQ=255)
      gcgTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGAt                                                                                                                                       >  1:266954/1‑151 (MQ=255)
       cgTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGAtt                                                                                                                                      >  1:181557/1‑151 (MQ=255)
           aTAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCa                                                                                                                                  <  2:136356/151‑1 (MQ=255)
                gATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAgg                                                                                                                             >  1:467580/1‑151 (MQ=255)
                      gACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCaa                                                                                                                       <  2:480778/151‑1 (MQ=255)
                       aCGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCa                                                                                                                        >  2:5805/1‑149 (MQ=255)
                           cATTGCCTTTACCGTTTCAACCCGTACCGGGAAACAGCTGCTGAAAGAGGCGGGCGACAGCAACATGAAACGCGTCTGTCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAgcg                                                                                                                    <  1:658826/149‑1 (MQ=255)
                                ccTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCgc                                                                                                             <  2:117313/151‑1 (MQ=255)
                                   ttACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCgca                                                                                                            <  1:581839/149‑1 (MQ=255)
                                           tCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCAtt                                                                                                   <  2:228754/150‑1 (MQ=255)
                                            cAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCAttt                                                                                                  <  1:564478/150‑1 (MQ=255)
                                                 cGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCa                                                                                                                        <  1:444507/123‑1 (MQ=255)
                                                 cGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCa                                                                                                                        >  2:444512/1‑123 (MQ=255)
                                                  gTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACaa                                                                                           <  1:126197/151‑1 (MQ=255)
                                                     ccGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAAcc                                                                                         >  1:489046/1‑150 (MQ=255)
                                                       gggAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAgg                                                                                      >  2:203065/1‑151 (MQ=255)
                                                           aaCAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGAc                                                                                   >  1:665412/1‑150 (MQ=255)
                                                           aaCAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACa                                                                                  <  2:608342/151‑1 (MQ=255)
                                                            aCAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAg                                                                                 <  2:247825/151‑1 (MQ=255)
                                                             cAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAgg                                                                                >  2:334723/1‑151 (MQ=255)
                                                                 tgctgAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGtgtg                                                                            >  2:417857/1‑151 (MQ=255)
                                                                   ctgAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGc                                                                           >  2:398419/1‑150 (MQ=255)
                                                                          aTGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCgg                                                                   <  2:173013/151‑1 (MQ=255)
                                                                              gggCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAAcg                                                               >  1:516553/1‑151 (MQ=255)
                                                                                       cAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTg                                                      <  1:485999/151‑1 (MQ=255)
                                                                                         aCATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCt                                                    <  1:298955/151‑1 (MQ=255)
                                                                                                aCGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAagag                                             <  2:588423/151‑1 (MQ=255)
                                                                                                aCGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAagag                                             <  2:540786/151‑1 (MQ=255)
                                                                                                    gTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCa                                         <  2:294516/151‑1 (MQ=255)
                                                                                                     tCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCAt                                        >  2:374695/1‑151 (MQ=255)
                                                                                                      ctggctggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATc                                       >  1:439857/1‑151 (MQ=255)
                                                                                                           tggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCg                                   >  2:653531/1‑150 (MQ=255)
                                                                                                            ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCg                                   <  1:508671/149‑1 (MQ=255)
                                                                                                            ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGAt                                 <  2:549239/151‑1 (MQ=255)
                                                                                                            ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGAt                                 <  2:516556/151‑1 (MQ=255)
                                                                                                                 cgggcggCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAAtt                            <  1:288163/151‑1 (MQ=255)
                                                                                                                 cgggcggCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAAtt                            >  2:648249/1‑151 (MQ=255)
                                                                                                                 cgggcggCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAAtt                            >  2:325757/1‑151 (MQ=255)
                                                                                                                   ggcggcAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCt                          <  1:346189/151‑1 (MQ=255)
                                                                                                                        cAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGcc                     >  1:128924/1‑151 (MQ=255)
                                                                                                                         aaaaGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGcct                    <  1:157284/151‑2 (MQ=255)
                                                                                                                         aaaaGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGccc                    <  2:303957/151‑1 (MQ=255)
                                                                                                                               gcCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTaa              <  2:310604/151‑1 (MQ=255)
                                                                                                                                  aaCATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAAc           >  1:425621/1‑151 (MQ=255)
                                                                                                                                  aaCATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAAc           >  2:667647/1‑151 (MQ=255)
                                                                                                                                        gTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAgg     <  2:407212/151‑1 (MQ=255)
                                                                                                                                          tttCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGgcg   >  1:259605/1‑151 (MQ=255)
                                                                                                                                           ttCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGgcgc  >  1:259011/1‑151 (MQ=255)
                                                                                                                                            tCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGgcgc  >  1:396105/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGC  >  NC_000913/1363432‑1363721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A