Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,370,155	A→G	intergenic (+152/‑61)	pspE → / → ycjM	thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,370,155	0	A	G	100.0%	142.4 / NA	38	intergenic (+152/‑61)	pspE/ycjM	thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/14); total (24/14)

AATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCT  >  NC_000913/1370007‑1370301

aaTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCa                                                                                                                                                  <  1:639479/151‑1 (MQ=255)

    cACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGttt                                                                                                                                              >  2:140604/1‑151 (MQ=255)

          gAGCGCCGCTTATGCGCCGTGTTTTTTTCTGGCAAACCTTATTTACATCCACATAGATTACTTGCGTCAGCTCACAAATACGGTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTAtttt                                                                                                                                         >  1:33107/1‑150 (MQ=255)

               gcgcTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCgg                                                                                                                                   <  2:373184/151‑1 (MQ=255)

                 gcTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCgg                                                                                                                                   >  1:114942/1‑149 (MQ=255)

                     aTGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGagtagt                                                                                                                             <  2:411938/151‑1 (MQ=255)

                         gcCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGtctct                                                                                                                         >  2:40189/1‑151 (MQ=255)

                              ttttttttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGttt                                                                                                                    >  2:448556/1‑151 (MQ=255)

                              ttttttttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGtt                                                                                                                     <  1:588531/150‑1 (MQ=255)

                                ttttttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTc                                                                                                                   >  1:327111/1‑150 (MQ=255)

                                   tttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATg                                                                                                                <  2:226744/150‑1 (MQ=255)

                                        gTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAc                                                                                                            <  1:379297/149‑1 (MQ=255)

                                                ttATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACg                                                                                                  >  1:617173/1‑151 (MQ=255)

                                                 tATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACgg                                                                                                 >  2:448579/1‑151 (MQ=255)

                                                              aTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                                                                     >  1:584642/1‑150 (MQ=255)

                                                                aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGaa                                                                                   >  2:389739/1‑150 (MQ=255)

                                                                         gCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAAtt                                                                         <  1:370418/151‑1 (MQ=255)

                                                                             cAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATGTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACg                                                                      <  1:341586/150‑1 (MQ=255)

                                                                               gCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAt                                                                   >  2:497580/1‑151 (MQ=255)

                                                                                 tCACAAATACGATTTTTCCCTGGTAAAAAATGATTTCCTGCTTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGTAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAtt                                                                  >  1:262422/1‑150 (MQ=255)

                                                                                          cGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACg                                                         >  1:561281/1‑150 (MQ=255)

                                                                                           gCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGa                                                        >  1:554695/1‑150 (MQ=255)

                                                                                                   ccTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACgg                                               >  2:632282/1‑151 (MQ=255)

                                                                                                    cTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGt                                              >  2:129178/1‑151 (MQ=255)

                                                                                                          aaaaaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACa                                        <  2:548647/151‑1 (MQ=255)

                                                                                                           aaaaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACAt                                       >  1:116758/1‑151 (MQ=255)

                                                                                                            aaaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACAtt                                      >  2:525276/1‑151 (MQ=255)

                                                                                                                 aTTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCg                                 <  1:240937/151‑1 (MQ=255)

                                                                                                                  tttCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGc                                >  2:309666/1‑151 (MQ=255)

                                                                                                                  tttCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGc                                >  2:610554/1‑151 (MQ=255)

                                                                                                                     ccTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCa                               <  1:521378/149‑1 (MQ=255)

                                                                                                                            gACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCAttt                       <  1:389735/150‑1 (MQ=255)

                                                                                                                            gACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCAttt                       <  1:129368/150‑1 (MQ=255)

                                                                                                                             aCTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTAc                     >  2:142078/1‑151 (MQ=255)

                                                                                                                                  aaCCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGaaa                <  2:620385/151‑1 (MQ=255)

                                                                                                                                       ttGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGCGGAACATTTACCGCAACTCATTTACAGAAACTTGt           >  1:652236/1‑151 (MQ=255)

                                                                                                                                           gCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAAcg       >  1:539396/1‑151 (MQ=255)

                                                                                                                                                aTTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCt  >  2:385085/1‑151 (MQ=255)

AATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCT  >  NC_000913/1370007‑1370301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A