Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,370,155	A→G	intergenic (+152/‑61)	pspE → / → ycjM	thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,370,155	0	A	G	100.0%	176.7 / NA	47	intergenic (+152/‑61)	pspE/ycjM	thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/20); total (27/20)

AATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCT  >  NC_000913/1370007‑1370301

aaTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCa                                                                                                                                                  <  2:492348/151‑1 (MQ=255)

aaTTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCa                                                                                                                                                  <  2:194185/151‑1 (MQ=255)

    cACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGttt                                                                                                                                              <  2:95755/151‑1 (MQ=255)

       cATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTAtt                                                                                                                                           >  2:417857/1‑151 (MQ=255)

           aGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCt                                                                                                                                       <  1:143170/151‑1 (MQ=255)

            gcggcgCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCt                                                                                                                                       >  2:594262/1‑150 (MQ=255)

                        cgcCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGtctc                                                                                                                          >  1:139739/1‑151 (MQ=255)

                           cGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCg                                                                                                                       <  1:571279/151‑1 (MQ=255)

                            gTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGt                                                                                                                      <  2:555116/151‑1 (MQ=255)

                                      cTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAc                                                                                                            >  1:125736/1‑151 (MQ=255)

                                        gTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAc                                                                                                            <  1:593801/149‑1 (MQ=255)

                                         tCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGc                                                                                                         >  1:528014/1‑151 (MQ=255)

                                             acCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTAcc                                                                                                     >  2:50270/1‑151 (MQ=255)

                                             acCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTAcc                                                                                                     >  2:460575/1‑151 (MQ=255)

                                              ccTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCa                                                                                                    <  1:530977/151‑1 (MQ=255)

                                                 tATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACg                                                                                                  <  2:171698/150‑1 (MQ=255)

                                                           cccATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAAc                                                                                        >  2:76619/1‑150 (MQ=255)

                                                            ccATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACAt                                                                                      >  2:155052/1‑151 (MQ=255)

                                                             cATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATg                                                                                     >  2:367504/1‑151 (MQ=255)

                                                                aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCATGGAAAGGCAACATGaa                                                                                   <  2:624213/150‑1 (MQ=255)

                                                                aGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGaa                                                                                   <  1:415978/150‑1 (MQ=255)

                                                                 gATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAAc                                                                                 <  2:26489/151‑1 (MQ=255)

                                                                        tGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGaaaa                                                                           <  1:194876/150‑1 (MQ=255)

                                                                                cTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAtt                                                                  >  2:602553/1‑151 (MQ=255)

                                                                                 tCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGAtt                                                                  >  1:545484/1‑150 (MQ=255)

                                                                                    caAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCt                                                              >  2:190542/1‑151 (MQ=255)

                                                                                        tACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGAc                                                          >  2:148781/1‑151 (MQ=255)

                                                                                         aCGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACg                                                         <  1:417864/151‑1 (MQ=255)

                                                                                              ttttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGaaa                                                      <  1:410368/149‑1 (MQ=255)

                                                                                              ttttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAAt                                                     <  2:400211/150‑1 (MQ=255)

                                                                                               tttCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCt                                                   >  2:466531/1‑151 (MQ=255)

                                                                                                     tGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTg                                             >  1:166604/1‑151 (MQ=255)

                                                                                                            aaaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACAtt                                      >  2:483479/1‑151 (MQ=255)

                                                                                                             aaTGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACAttt                                     >  1:398985/1‑151 (MQ=255)

                                                                                                               tGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAc                                   >  1:359012/1‑151 (MQ=255)

                                                                                                               tGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAc                                   >  1:557272/1‑151 (MQ=255)

                                                                                                                gATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAcc                                  >  2:552359/1‑151 (MQ=255)

                                                                                                                 aTTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTAcc                                  <  2:515864/150‑1 (MQ=255)

                                                                                                                 aTTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCg                                 >  2:399146/1‑151 (MQ=255)

                                                                                                                   ttCCTGCGTGACTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGc                                <  2:506586/150‑1 (MQ=255)

                                                                                                                             aCTAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTAc                     <  2:250184/151‑1 (MQ=255)

                                                                                                                               tAAAACCCTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTAc                     >  2:90687/1‑149 (MQ=255)

                                                                                                                                      cTTGTGCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTg            >  2:433098/1‑151 (MQ=255)

                                                                                                                                         gtgCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTaa         <  1:261647/151‑1 (MQ=255)

                                                                                                                                         gtgCTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTa          >  2:146223/1‑150 (MQ=255)

                                                                                                                                            cTCAATTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAAcgc      >  1:557317/1‑151 (MQ=255)

                                                                                                                                                aTTGGCAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCt  >  2:75430/1‑151 (MQ=255)

AATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGACCGCTACCACGGAAAGGCAACATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAACGCGTCT  >  NC_000913/1370007‑1370301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A