Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,185	G→A	G87S (GGC→AGC)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,185	0	G	A	100.0%	133.8 / NA	35	G87S (GGC→AGC)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/18); total (17/18)

CATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAAT  >  NC_000913/1454038‑1454322

caTGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCt                                                                                                                                        <  1:332067/151‑1 (MQ=255)

    cgcACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTgcgc                                                                                                                                    >  2:449484/1‑151 (MQ=255)

           cGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATCCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAgaga                                                                                                                              >  2:20325/1‑150 (MQ=255)

            gagaTTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGagaag                                                                                                                            <  2:146154/151‑1 (MQ=255)

                 tgttgGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATc                                                                                                                       >  2:221707/1‑151 (MQ=255)

                    tgGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTa                                                                                                                     <  2:362227/150‑1 (MQ=255)

                    tgGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTa                                                                                                                     <  2:179513/150‑1 (MQ=255)

                                    ggTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacg                                                                                                    <  1:400088/151‑1 (MQ=255)

                                     gTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATg                                                                                                           <  2:641641/143‑1 (MQ=255)

                                     gTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATg                                                                                                           >  1:641631/1‑143 (MQ=255)

                                       aaCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacggac                                                                                                 >  1:513294/1‑151 (MQ=255)

                                       aaCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacgga                                                                                                  <  1:197537/150‑1 (MQ=255)

                                       aaCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacgga                                                                                                  <  1:570880/150‑1 (MQ=255)

                                        aCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacggacg                                                                                                <  1:403094/151‑1 (MQ=255)

                                             aTCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGa                                                                                           <  1:253303/151‑1 (MQ=255)

                                                     cgcACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATtcc                                                                                   >  1:489229/1‑151 (MQ=255)

                                                           aaCCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATc                                                                             >  1:48465/1‑151 (MQ=255)

                                                                tGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTa                                                                         >  1:492561/1‑150 (MQ=255)

                                                                tGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTa                                                                         <  1:562369/150‑1 (MQ=255)

                                                                              aTTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTg                                                           >  1:304048/1‑150 (MQ=255)

                                                                                tCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAg                                                         <  1:151868/150‑1 (MQ=255)

                                                                                   gTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTg                                                      <  1:449479/150‑1 (MQ=255)

                                                                                          aaaGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGata                                              <  1:20326/151‑1 (MQ=255)

                                                                                          aaaGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGat                                               >  1:602652/1‑150 (MQ=255)

                                                                                                            ggTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggctggc                            >  1:107164/1‑151 (MQ=255)

                                                                                                              tCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGgctggctg                          >  1:37754/1‑151 (MQ=255)

                                                                                                                aTGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGctggctgg                         >  2:547571/1‑150 (MQ=255)

                                                                                                                  ggTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCtggtgg                      >  1:395854/1‑151 (MQ=255)

                                                                                                                  ggTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCtggtg                       <  1:522578/150‑1 (MQ=255)

                                                                                                                       gTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATgg                  <  2:199083/150‑1 (MQ=255)

                                                                                                                          tctcTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGgcgc               >  1:526997/1‑150 (MQ=255)

                                                                                                                               ataGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGc            <  1:516517/148‑1 (MQ=255)

                                                                                                                                taGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATc        >  1:461097/1‑151 (MQ=255)

                                                                                                                                      cgccgAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAAt  >  2:326627/1‑151 (MQ=255)

                                                                                                                                          gAAACTCTGAGCAGCGACAGAGAAGTCATCTACCAAAAAATGCTCGACGGAGGGGTGAGATGTTCCTCGTTCTTTAGTTATCCGGCAGTGAGGTGGGCTGATATCAGTGTTATCGGCTGGCTGGTGGATGGCCTAGTGATCGt      <  1:156101/143‑1 (MQ=255)

CATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAAT  >  NC_000913/1454038‑1454322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A