Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,483,905	T→C	V282A (GTA→GCA)	hrpA →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,483,905	0	T	C	100.0%	111.3 / NA	35	V282A (GTA→GCA)	hrpA	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/18); total (17/18)

CCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACA  >  NC_000913/1483760‑1484048

ccGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACg                                                                                                                                            >  2:504022/1‑151 (MQ=255)

        cTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGt                                                                                                                                    >  2:510514/1‑151 (MQ=255)

                      ttAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATg                                                                                                                       <  1:646843/150‑1 (MQ=255)

                        aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGc                                                                                                                     <  1:181999/150‑1 (MQ=255)

                        aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCg                                                                                                                    <  1:376975/151‑1 (MQ=255)

                        aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCg                                                                                                                    <  1:619608/151‑1 (MQ=255)

                        aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGGGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCg                                                                                                                    >  1:135010/1‑151 (MQ=255)

                              gcgcCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTc                                                                                                              >  2:555509/1‑151 (MQ=255)

                                      tattGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCttt                                                                                                      >  1:216950/1‑151 (MQ=255)

                                        ttGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTAt                                                                                                    >  1:261309/1‑151 (MQ=255)

                                           aaGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATg                                                                                                   >  1:155289/1‑149 (MQ=255)

                                           aaGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAg                                                                                                 <  1:436868/151‑1 (MQ=255)

                                                                       aGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATAccgcc                                                                     <  2:434093/151‑1 (MQ=255)

                                                                             cTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgc                                                                >  2:458359/1‑150 (MQ=255)

                                                                              tATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgcgc                                                              >  2:636930/1‑151 (MQ=255)

                                                                               aTCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgcg                                                               <  2:384609/149‑1 (MQ=255)

                                                                               aTCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCt                                                             <  2:314940/151‑1 (MQ=255)

                                                                                  gCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGa                                                           >  1:483378/1‑150 (MQ=255)

                                                                                   cccGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAAc                                                         <  2:155292/151‑1 (MQ=255)

                                                                                    ccGATTGTTGAAGAAGCCGATGACCCCGGGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTAGGAGCGGCGAGCGGAAAATCCGCGATACCGCCGATGCGCTGAACa                                                        <  1:251062/151‑1 (MQ=255)

                                                                                        ttgttgAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCt                                                    >  2:177323/1‑151 (MQ=255)

                                                                                                   gCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGc                                          <  2:621479/150‑1 (MQ=255)

                                                                                                             ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGaaa                                <  1:579237/150‑1 (MQ=255)

                                                                                                             ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAAt                               <  2:657541/151‑1 (MQ=255)

                                                                                                             ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAAt                               <  2:216951/151‑1 (MQ=255)

                                                                                                             ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAAt                               <  1:510511/151‑1 (MQ=255)

                                                                                                                   gcgATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGcc                         >  2:373078/1‑151 (MQ=255)

                                                                                                                      aTCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGc                       <  1:396604/150‑1 (MQ=255)

                                                                                                                      aTCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCt                      <  1:555504/151‑1 (MQ=255)

                                                                                                                          gTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTAt                  >  2:389782/1‑151 (MQ=255)

                                                                                                                             gCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATgcg               >  1:218340/1‑151 (MQ=255)

                                                                                                                                 gCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGc           >  2:317329/1‑151 (MQ=255)

                                                                                                                                          gACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACa  <  2:270584/151‑1 (MQ=255)

                                                                                                                                           aCGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGCAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAAc   >  1:39432/1‑149 (MQ=255)

                                                                                                                                           aCGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAAc   >  1:206688/1‑149 (MQ=255)

CCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACA  >  NC_000913/1483760‑1484048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A