Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,754,748	A→G	intergenic (‑271/+184)	ydhY ← / ← ydhZ	putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,754,748	0	A	G	100.0%	191.2 / NA	49	intergenic (‑271/+184)	ydhY/ydhZ	putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/20); total (29/20)

AGAAAAGATTAACGCCGATAAACTAATCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATAAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACTGAAG  >  NC_000913/1754602‑1754890

agaAAAGATTAACGCCGATAAACTAATCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAAtt                                                                                                                                            >  2:78879/1‑151 (MQ=255)

  aaaaGATTAACGCCGATAAACTAATCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTa                                                                                                                                          <  1:409499/151‑1 (MQ=255)

        ttAACGCCGATAAACTAATCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTa                                                                                                                                     >  2:396447/1‑150 (MQ=255)

                   aaaCTAATCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAattat                                                                                                                         >  2:186780/1‑151 (MQ=255)

                        aaTCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTtgtg                                                                                                                    <  1:565987/151‑1 (MQ=255)

                         aTCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTtgtg                                                                                                                    >  1:502341/1‑150 (MQ=255)

                                 tGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCAtt                                                                                                           >  2:494303/1‑151 (MQ=255)

                                  ggTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTg                                                                                                          >  1:560858/1‑151 (MQ=255)

                                   gTTAGGAGAAAAAATAATGTGATTATTCTGAGCGGTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAACTTAACTTAAGCGACAATTATTTGTGACTTTCATTGc                                                                                                         <  2:221551/151‑1 (MQ=255)

                                    ttAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGc                                                                                                         >  2:581342/1‑150 (MQ=255)

                                     tAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGc                                                                                                         >  2:174283/1‑149 (MQ=255)

                                     tAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCa                                                                                                        <  1:513858/150‑1 (MQ=255)

                                          gaAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGaaaat                                                                                                  >  1:100513/1‑151 (MQ=255)

                                           aaaaaaTAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGaaaat                                                                                                  >  1:458544/1‑150 (MQ=255)

                                             aaaaTAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGaaaataa                                                                                                >  1:443265/1‑150 (MQ=255)

                                              aaaTAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGaaaataaaa                                                                                              <  2:181502/151‑1 (MQ=255)

                                                   atGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAAc                                                                                         >  1:392817/1‑151 (MQ=255)

                                                            ttCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCt                                                                                >  2:285495/1‑151 (MQ=255)

                                                             tCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCt                                                                                <  1:488628/150‑1 (MQ=255)

                                                                  gCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAAt                                                                          <  1:582172/151‑1 (MQ=255)

                                                                    ccTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAAtt                                                                         >  2:425149/1‑150 (MQ=255)

                                                                    ccTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTa                                                                        <  1:413734/151‑1 (MQ=255)

                                                                     cTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTa                                                                        >  1:555869/1‑150 (MQ=255)

                                                                      ttAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTaa                                                                       >  1:16654/1‑150 (MQ=255)

                                                                       tAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTaaa                                                                      <  1:254762/150‑1 (MQ=255)

                                                                        aaCATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTaaaa                                                                     <  2:389931/150‑1 (MQ=255)

                                                                         aCATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAat                                                                    >  1:451326/1‑150 (MQ=255)

                                                                               aTCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATAcc                                                             <  1:499768/151‑1 (MQ=255)

                                                                                  gTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGaa                                                          <  1:581639/151‑1 (MQ=255)

                                                                                   ttATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGaa                                                          >  2:538936/1‑150 (MQ=255)

                                                                                      tCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTc                                                      >  1:185755/1‑151 (MQ=255)

                                                                                                 tACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTc                                            <  2:661782/150‑1 (MQ=255)

                                                                                                    aaaGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTcagc                                         >  2:194729/1‑150 (MQ=255)

                                                                                                    aaaGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTcagc                                         >  1:411930/1‑150 (MQ=255)

                                                                                                     aaGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTcagc                                         >  2:207525/1‑149 (MQ=255)

                                                                                                      aGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAgt                                      >  2:407897/1‑151 (MQ=255)

                                                                                                         aaGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAgttgt                                   >  2:493517/1‑151 (MQ=255)

                                                                                                           gAAATTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTa                                 <  1:481985/151‑1 (MQ=255)

                                                                                                           gAAATTATAAGGGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAgttgtt                                  >  2:545187/1‑150 (MQ=255)

                                                                                                              aTTATAAGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTAttt                              >  2:318291/1‑151 (MQ=255)

                                                                                                                    aGTGAACTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATg                        <  1:216299/151‑1 (MQ=255)

                                                                                                                        aaCTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGAtt                     >  1:29315/1‑150 (MQ=255)

                                                                                                                        aaCTGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTa                    <  1:194728/151‑1 (MQ=255)

                                                                                                                           tGATATTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACt                 >  1:501375/1‑151 (MQ=255)

                                                                                                                             atatTTATTATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTg                <  1:418253/150‑1 (MQ=255)

                                                                                                                               atttattATCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGa              <  1:407060/150‑1 (MQ=255)

                                                                                                                                   attatCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGc          <  1:373293/150‑1 (MQ=255)

                                                                                                                                      atCATTTGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACt      >  2:123066/1‑151 (MQ=255)

                                                                                                                                           ttGAAATGAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACTGAAg  <  2:174431/150‑1 (MQ=255)

AGAAAAGATTAACGCCGATAAACTAATCCTTTATGGTTAGGAGAAAAAATAATGTGATTATTCTGAGCCCTTAACATTGATCGTTATCAATTAAAATTACAAAGCAAGAAATTATAAGTGAACTGATATTTATTATCATTTGAAATAAATTTAACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACTGAAG  >  NC_000913/1754602‑1754890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A