Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,078	T→C	intergenic (‑58/+189)	ydiV ← / ← nlpC	anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,078	0	T	C	100.0%	129.2 / NA	36	intergenic (‑58/+189)	ydiV/nlpC	anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/14); total (22/14)

ACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGG  >  NC_000913/1791933‑1792221

aCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGAc                                                                                                                                             >  1:475647/1‑151 (MQ=255)

 cTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGAc                                                                                                                                             >  1:328443/1‑150 (MQ=255)

   cAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACAt                                                                                                                                           >  2:388389/1‑150 (MQ=255)

       gCCGACAAGAACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCATCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACaa                                                                                                                                      <  1:529365/151‑1 (MQ=255)

         cGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAAtt                                                                                                                                    <  2:150787/151‑1 (MQ=255)

         cGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAAtt                                                                                                                                    >  1:616891/1‑151 (MQ=255)

             aaGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTAc                                                                                                                                  >  2:64883/1‑149 (MQ=255)

                           tGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACttt                                                                                                                   <  1:212417/150‑1 (MQ=255)

                                ctTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCAtt                                                                                                               <  2:459627/149‑1 (MQ=255)

                                 ttATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGt                                                                                                            >  1:316487/1‑151 (MQ=255)

                                  tATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGt                                                                                                            <  1:116861/150‑1 (MQ=255)

                                         aGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGtt                                                                                                    >  2:636883/1‑151 (MQ=255)

                                               tAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCAc                                                                                              >  2:404137/1‑151 (MQ=255)

                                                        gAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAgg                                                                                     <  1:602498/151‑1 (MQ=255)

                                                            gATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTc                                                                                 >  1:577215/1‑151 (MQ=255)

                                                               aaaGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑tt                                                                             <  2:494145/151‑1 (MQ=255)

                                                                      ctcCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGATAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑tttttt                                                                         >  1:210675/1‑148 (MQ=255)

                                                                        ccAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑ttttttt                                                                        >  2:461670/1‑147 (MQ=255)

                                                                         cAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGAtt                                                                   >  2:371776/1‑151 (MQ=255)

                                                                            aaaaaTCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATc                                                                >  2:633308/1‑151 (MQ=255)

                                                                              aaaTCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCt                                                              >  1:503721/1‑151 (MQ=255)

                                                                                    tCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGa                                                            <  2:317416/147‑1 (MQ=255)

                                                                                           aaCCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaa                                                 <  1:449171/151‑1 (MQ=255)

                                                                                             ccGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaata                                               <  2:328445/151‑1 (MQ=255)

                                                                                               gTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaataat                                             >  2:487027/1‑151 (MQ=255)

                                                                                               gTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaataat                                             >  1:369452/1‑151 (MQ=255)

                                                                                                  ccTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTTAGATTATCCTGATTATAAACGAATAATcc                                           >  1:179910/1‑151 (MQ=255)

                                                                                                  ccTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATc                                            >  1:280928/1‑149 (MQ=255)

                                                                                                               gTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAAttt                              <  1:404133/150‑1 (MQ=255)

                                                                                                               gTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAAttt                              <  1:210833/150‑1 (MQ=255)

                                                                                                                         aTCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCaa                    >  2:374206/1‑150 (MQ=255)

                                                                                                                             ggCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtac               >  2:435906/1‑151 (MQ=255)

                                                                                                                              gCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtact              >  2:187189/1‑151 (MQ=255)

                                                                                                                              gCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtact              <  1:89705/151‑1 (MQ=255)

                                                                                                                                      ggATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTAtt      >  1:60852/1‑151 (MQ=255)

                                                                                                                                          gAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAgg  <  1:87137/151‑1 (MQ=255)

ACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGG  >  NC_000913/1791933‑1792221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A