Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,861,807	T→C	A56A (GCA→GCG)	yeaC ←	DUF1315 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,807	0	T	C	100.0%	140.8 / NA	39	A56A (GCA→GCG)	yeaC	DUF1315 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/21); total (18/21)

GGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAG  >  NC_000913/1861660‑1861940

ggCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTgcgc                                                                                                                                     <  2:454607/150‑1 (MQ=255)

    aTCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCg                                                                                                                                 <  2:357636/150‑1 (MQ=255)

      ccccTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGat                                                                                                                               >  2:381429/1‑150 (MQ=255)

        ccTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGAtatt                                                                                                                            >  1:151988/1‑151 (MQ=255)

            cATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATg                                                                                                                         <  1:321532/150‑1 (MQ=255)

             aTAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGc                                                                                                                       >  1:614309/1‑151 (MQ=255)

              tAGCCGAGGCAAAAAGGGTATGAACCACTTACTTAAACATTGCAATAGGCTTTGCTCAGATACCAAAATCTTCTTTTAACTGCTGTTTGTTCTTCATGACCATCTGACCATTTGTGTCAATTTTCATATGCTGCGATTCGAtattat                                                                                                                          <  1:192621/147‑1 (MQ=255)

              tAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCg                                                                                                                      >  2:489661/1‑151 (MQ=255)

               aGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCgg                                                                                                                     <  1:226615/151‑1 (MQ=255)

               aGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCg                                                                                                                      <  1:549584/150‑1 (MQ=255)

                  cGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCt                                                                                                                  <  2:234221/151‑1 (MQ=255)

                   gAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGGtt                                                                                                                 >  2:138580/1‑151 (MQ=255)

                   gAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCtt                                                                                                                 <  1:190803/151‑1 (MQ=255)

                   gAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCtt                                                                                                                 <  1:240953/151‑1 (MQ=255)

                         aaaaGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCcaca                                                                                                           <  1:186627/151‑1 (MQ=255)

                          aaaGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGttttttac                                                                                                            >  1:105925/1‑144 (MQ=255)

                               ggATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCa                                                                                                     <  2:339353/151‑1 (MQ=255)

                                 aTGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGTCACAGCATCaac                                                                                                   >  2:354378/1‑149 (MQ=255)

                                     aCCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGtt                                                                                               <  2:375097/151‑1 (MQ=255)

                                                       gCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGTCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCttttt                                                                             <  2:196913/151‑1 (MQ=255)

                                                          aTAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGCTGCAGGCAGTTCTCtttttttt                                                                          >  2:49629/1‑151 (MQ=255)

                                                                 ttGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTTCCGGCCGTTCTTTTTTTTTTtctc                                                                      >  1:79096/1‑148 (MQ=255)

                                                                  tGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGt                                                                   <  2:364553/150‑1 (MQ=255)

                                                                   gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTca                                                                 <  2:283060/151‑1 (MQ=255)

                                                                     tGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCCTACCCCGTTGGCGGCAGTTTTCTTTTTTTTTCTCGGTcaa                                                                >  2:10786/1‑150 (MQ=255)

                                                                      gagaTACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaac                                                               <  2:251094/150‑1 (MQ=255)

                                                                      gagaTACCAAAATCTTCCTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaac                                                               <  2:89899/150‑1 (MQ=255)

                                                                              aaaaTCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCACCCTCAACCCTTTCGGGCAGTTTTCTTTTTTTTTCTCCGTccaccccacccc                                                       >  1:113452/1‑142 (MQ=255)

                                                                               aaaTCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGTTGCAGGCAGGTCTCTTTTTTTTCCTCGGTCAACGCAACGcc                                                       >  2:507331/1‑149 (MQ=255)

                                                                                       ttttAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAATTGCAGGCCGTTCTCTTTTTTTTCCCCCGTCAACCCCACCCCCTCAAGcc                                               >  1:136643/1‑149 (MQ=255)

                                                                                        tttAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCAt                                             <  1:610135/150‑1 (MQ=255)

                                                                                            aCTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGGTTGCCACAGCATCACCCAGTGTAGGGCGGTTTTTTTTTTTTCCTCCGGCCACACAACGCAATCAAGCCACTTtcc                                        >  2:581942/1‑151 (MQ=255)

                                                                                                   tttGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGCTGGAGGCAGGTCTTTTTTTTTTTCTCGGTCAACGCAACCCCATCAGGCCATTTCCCCCGtt                                   >  2:385916/1‑149 (MQ=255)

                                                                                                    ttGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCa                                 <  2:50325/150‑1 (MQ=255)

                                                                                                         ctTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTTCTCGGGCAACGCAACGCCATCAGGCCATTTCCCCCGTTCAAcggcg                           >  2:16070/1‑151 (MQ=255)

                                                                                                             aTGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTca                       <  1:526116/151‑2 (MQ=255)

                                                                                                                     cTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTTCTCGGTCAAAGCAACGCCATCAGGCCATTTCCCCACGTCAACGGCGGGCGACaaa                  >  2:4013/1‑148 (MQ=255)

                                                                                                                      tGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTCCTCGGGCCACGCAACGCCATCCGGCCATTTCCCCAGTTCAACGGCGGGCGCCaaaacc               >  2:26161/1‑147 (MQ=255)

                                                                                                                       gACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTg             <  1:424314/151‑1 (MQ=255)

                                                                                                                                   cAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAg  <  2:115528/150‑1 (MQ=255)

GGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAG  >  NC_000913/1861660‑1861940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A