Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,956,520	T→C	N163S (AAC→AGC)	torZ ←	trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,956,520	0	T	C	100.0%	144.8 / NA	39	N163S (AAC→AGC)	torZ	trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/22); total (17/22)

CAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTCCCA  >  NC_000913/1956372‑1956665

caGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTc                                                                                                                                                 >  2:537252/1‑151 (MQ=255)

         aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGt                                                                                                                                        <  2:433267/151‑1 (MQ=255)

           ccTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGtt                                                                                                                                       <  2:322057/150‑1 (MQ=255)

                  gTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTa                                                                                                                                 <  1:522740/149‑1 (MQ=255)

                     ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGtt                                                                                                                            >  2:210691/1‑151 (MQ=255)

                            aTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCtt                                                                                                                     <  2:38314/151‑1 (MQ=255)

                             tCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCttt                                                                                                                    >  2:612981/1‑151 (MQ=255)

                              cAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTa                                                                                                                   <  1:480389/151‑1 (MQ=255)

                                 cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGc                                                                                                                >  2:370876/1‑151 (MQ=255)

                                     cAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAaca                                                                                                            <  2:534391/151‑1 (MQ=255)

                                        gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGc                                                                                                         >  1:117782/1‑151 (MQ=255)

                                        gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGc                                                                                                         >  1:55604/1‑151 (MQ=255)

                                          ggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGcc                                                                                                        >  2:541938/1‑150 (MQ=255)

                                           gTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGcc                                                                                                        >  2:287685/1‑149 (MQ=255)

                                                    tCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGcc                                                                                             >  1:519154/1‑151 (MQ=255)

                                                           ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTa                                                                                      <  1:237612/151‑1 (MQ=255)

                                                                    aaCCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGc                                                                             >  2:276040/1‑151 (MQ=255)

                                                                      ccGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCa                                                                            >  1:503745/1‑150 (MQ=255)

                                                                      ccGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCa                                                                            <  2:32641/150‑1 (MQ=255)

                                                                          ccACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCaaaaa                                                                        <  1:399594/150‑1 (MQ=255)

                                                                          ccACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAAt                                                                       >  2:224028/1‑151 (MQ=255)

                                                                             caTGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATgg                                                                     <  1:210690/150‑1 (MQ=255)

                                                                                gCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGcc                                                                   <  1:87821/149‑1 (MQ=255)

                                                                                          cACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGtt                                                        <  1:52833/150‑1 (MQ=255)

                                                                                          cACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGtt                                                        <  2:468442/150‑1 (MQ=255)

                                                                                          cACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTa                                                       <  2:434860/151‑1 (MQ=255)

                                                                                                  ctgcCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACg                                               >  2:41377/1‑151 (MQ=255)

                                                                                                    gcCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGa                                              <  1:146797/150‑1 (MQ=255)

                                                                                                           ttGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATc                                      >  2:39685/1‑151 (MQ=255)

                                                                                                                         cTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGa                         >  1:319314/1‑150 (MQ=255)

                                                                                                                             gCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTT‑CGAATACGATCATGTTGCTCATGAAt                       <  2:425382/147‑1 (MQ=255)

                                                                                                                              cccGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGt                   <  2:520405/151‑1 (MQ=255)

                                                                                                                               ccGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGtt                  <  2:654028/151‑1 (MQ=255)

                                                                                                                                 ggAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGttt                 <  1:252540/150‑1 (MQ=255)

                                                                                                                                     tAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATg            >  2:484659/1‑151 (MQ=255)

                                                                                                                                         cgcccgccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTg        <  2:357788/151‑1 (MQ=255)

                                                                                                                                           cccgccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGtt      <  1:28766/151‑1 (MQ=255)

                                                                                                                                               ccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTccc   >  1:609273/1‑150 (MQ=255)

                                                                                                                                               ccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTCCCa  <  1:224028/151‑1 (MQ=255)

CAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTCCCA  >  NC_000913/1956372‑1956665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A