Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,136,954	A→G	V97A (GTC→GCC)	wza ←	colanic acid export protein; outer membrane auxillary lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,136,954	0	A	G	100.0%	114.5 / NA	33	V97A (GTC→GCC)	wza	colanic acid export protein; outer membrane auxillary lipoprotein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/15); total (18/15)

CGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACAT  >  NC_000913/2136820‑2137092

cgagcgTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAAc                                                                                                                            >  1:659637/1‑151 (MQ=255)

     gTTTTCCCGACTACGTGGACCTTGCCGTTATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTccc                                                                                                                       >  2:585187/1‑151 (MQ=255)

       tttCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTccccc                                                                                                                     >  2:237434/1‑151 (MQ=255)

         tCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCgg                                                                                                                   >  2:506940/1‑151 (MQ=255)

                  cGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGAcgcg                                                                                                          <  2:82937/151‑1 (MQ=255)

                   gTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGGGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGAcg                                                                                                            >  1:345762/1‑148 (MQ=255)

                     ggACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATa                                                                                                       <  1:190511/151‑1 (MQ=255)

                           tGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGt                                                                                                  <  2:474385/150‑1 (MQ=255)

                              cGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAAtt                                                                                              <  2:447986/151‑1 (MQ=255)

                              cGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAAtt                                                                                              >  2:252125/1‑151 (MQ=255)

                               gATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTc                                                                                             <  2:87000/151‑1 (MQ=255)

                                    aCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGa                                                                                         <  2:312110/150‑1 (MQ=255)

                                    aCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGAt                                                                                        >  2:600620/1‑151 (MQ=255)

                                     cGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGAtc                                                                                       >  2:148884/1‑151 (MQ=255)

                                          aaaaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACt                                                                                  >  1:549032/1‑151 (MQ=255)

                                              cATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTcc                                                                              <  1:655730/151‑1 (MQ=255)

                                                 aGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGc                                                                           >  2:189347/1‑151 (MQ=255)

                                                 aGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGGCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGc                                                                           >  1:248690/1‑151 (MQ=255)

                                                     ccGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCa                                                                       <  1:626883/151‑1 (MQ=255)

                                                      cGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCat                                                                      >  2:649200/1‑151 (MQ=255)

                                                          aGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCatat                                                                    >  1:320277/1‑149 (MQ=255)

                                                                tACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGgcgc                                                            >  1:263903/1‑151 (MQ=255)

                                                                           cGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgg                                                 >  2:406819/1‑151 (MQ=255)

                                                                            ggTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCggg                                                <  1:628166/151‑1 (MQ=255)

                                                                                    tGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgggcg                                        >  2:577080/1‑151 (MQ=255)

                                                                                     ggAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgggcgt                                       <  2:248691/151‑1 (MQ=255)

                                                                                        gCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGAGCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAAt                                    <  2:359536/151‑1 (MQ=255)

                                                                                                    aCCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAgg                        <  1:111575/151‑1 (MQ=255)

                                                                                                       ggCTGGCGTGGTGAGTTCCGGGGGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCCCTACATTCGGGCGTGGGCGTAATTGGGCAATCAGgcg                      >  1:143166/1‑150 (MQ=255)

                                                                                                          tGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCggg                  >  1:310556/1‑151 (MQ=255)

                                                                                                           ggCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCgg                   <  1:520315/149‑1 (MQ=255)

                                                                                                                    aGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCATCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGAt        <  1:531524/151‑1 (MQ=255)

                                                                                                                          gggTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACAt  <  2:421811/151‑1 (MQ=255)

CGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACAT  >  NC_000913/2136820‑2137092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A