Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,224,908 C→T intergenic (‑38/+136) pbpG ← / ← yohC D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,224,9080CT100.0% 188.3 / NA 48intergenic (‑38/+136)pbpG/yohCD‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/32);  total (16/32)

TTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAA  >  NC_000913/2224760‑2225049
                                                                                                                                                    |                                                                                                                                             
ttCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTgtgc                                                                                                                                             >  1:411726/1‑151 (MQ=255)
  ccGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAc                                                                                                                                           <  2:595934/151‑1 (MQ=255)
     gTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAccgc                                                                                                                                        >  2:593182/1‑151 (MQ=255)
          gAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGa                                                                                                                                    <  1:267793/150‑1 (MQ=255)
           aaGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGt                                                                                                                                  <  1:84308/151‑1 (MQ=255)
               ggtggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAgg                                                                                                                              <  1:431957/151‑1 (MQ=255)
                      gCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACt                                                                                                                       <  1:268232/151‑1 (MQ=255)
                        ggCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTg                                                                                                                      >  1:199338/1‑150 (MQ=255)
                        ggCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTg                                                                                                                      >  2:107516/1‑150 (MQ=255)
                             ccGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTa                                                                                                                <  2:443620/151‑1 (MQ=255)
                             ccGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTa                                                                                                                <  1:318052/151‑1 (MQ=255)
                             ccGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTa                                                                                                                <  1:409464/151‑1 (MQ=255)
                               gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                                                                                                              <  1:394570/151‑1 (MQ=255)
                                        aCGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatc                                                                                                     >  2:56073/1‑151 (MQ=255)
                                          ggCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatcat                                                                                                   >  2:557889/1‑151 (MQ=255)
                                            ccTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATaa                                                                                                 <  1:613925/151‑1 (MQ=255)
                                               gCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAAc                                                                                                <  2:652588/149‑1 (MQ=255)
                                                cGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAg                                                                                             <  2:244489/151‑1 (MQ=255)
                                                         aGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg                                                                                    <  2:359974/151‑1 (MQ=255)
                                                         aGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg                                                                                    <  2:549174/151‑1 (MQ=255)
                                                          ggCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGc                                                                                   <  1:425611/151‑1 (MQ=255)
                                                                gccagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGt                                                                             <  1:668756/151‑1 (MQ=255)
                                                                gccagcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGt                                                                             <  1:522865/151‑1 (MQ=255)
                                                                             ccAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCg                                                                <  2:457586/151‑1 (MQ=255)
                                                                                   taaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                           >  2:559447/1‑150 (MQ=255)
                                                                                    aaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGa                                                          >  2:434941/1‑150 (MQ=255)
                                                                                    aaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGa                                                          >  2:582363/1‑150 (MQ=255)
                                                                                      ataaaGAAACGCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAt                                                       <  1:500994/151‑1 (MQ=255)
                                                                                            aaaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgctgc                                                  >  1:376888/1‑150 (MQ=255)
                                                                                            aaaCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgctgc                                                  >  1:295459/1‑150 (MQ=255)
                                                                                              aCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAtt                                               <  2:552826/151‑1 (MQ=255)
                                                                                              aCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAtt                                               <  2:389124/151‑1 (MQ=255)
                                                                                                 cGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCt                                            <  1:5082/151‑1 (MQ=255)
                                                                                                      tttCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAt                                       >  1:297941/1‑151 (MQ=255)
                                                                                                          ggCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTAc                                   <  1:107513/151‑1 (MQ=255)
                                                                                                           gCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACg                                  >  1:370302/1‑151 (MQ=255)
                                                                                                             atgatgAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGcc                                >  1:156519/1‑151 (MQ=255)
                                                                                                                atgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGc                             <  2:270670/151‑1 (MQ=255)
                                                                                                                  gaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCa                            >  1:384302/1‑150 (MQ=255)
                                                                                                                    gCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAAt                          <  1:184101/150‑1 (MQ=255)
                                                                                                                     cATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGc                        <  2:452806/151‑1 (MQ=255)
                                                                                                                     cATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGc                        <  1:93571/151‑1 (MQ=255)
                                                                                                                      aTTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGc                        >  1:141512/1‑150 (MQ=255)
                                                                                                                      aTTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCa                       <  2:119210/151‑1 (MQ=255)
                                                                                                                         cAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAAc                     <  2:534356/150‑1 (MQ=255)
                                                                                                                             tAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCa                 >  1:81731/1‑150 (MQ=255)
                                                                                                                               gTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAg              <  1:171090/151‑1 (MQ=255)
                                                                                                                                       cgcgCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt      <  2:471841/151‑1 (MQ=255)
                                                                                                                                           cACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGaa  <  2:299293/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAA  >  NC_000913/2224760‑2225049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A