Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,460,130	T→C	M114V (ATG→GTG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,130	0	T	C	100.0%	122.0 / NA	34	M114V (ATG→GTG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/20); total (14/20)

GCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCATCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTAC  >  NC_000913/2459984‑2460268

gCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGTCACCAgg                                                                                                                                        <  2:481831/151‑1 (MQ=255)

  gagagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCt                                                                                                                                      >  1:328532/1‑151 (MQ=255)

    gagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCttt                                                                                                                                    <  1:596218/151‑1 (MQ=255)

           gTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGAc                                                                                                                             >  2:390231/1‑151 (MQ=255)

              tCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGtt                                                                                                                          >  1:432914/1‑151 (MQ=255)

                           cATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTg                                                                                                              <  1:364882/150‑1 (MQ=255)

                            aTGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTgg                                                                                                             >  2:555535/1‑150 (MQ=255)

                                  cGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTgg                                                                                                      >  2:64416/1‑151 (MQ=255)

                                        ttgttgACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAgc                                                                                                  <  1:230558/149‑1 (MQ=255)

                                                  cAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCt                                                                                      <  1:205143/151‑1 (MQ=255)

                                                       aGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGc                                                                                 >  1:23738/1‑151 (MQ=255)

                                                       aGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGc                                                                                 >  2:295424/1‑151 (MQ=255)

                                                       aGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGc                                                                                 >  2:224839/1‑151 (MQ=255)

                                                                  cAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCg                                                                      <  1:64416/151‑1 (MQ=255)

                                                                     ttttttACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGt                                                                    >  1:407296/1‑150 (MQ=255)

                                                                       ttttACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTAt                                                                  <  1:495262/150‑1 (MQ=255)

                                                                          tACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTa                                                               <  2:592720/150‑1 (MQ=255)

                                                                                 gCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCATGCTTTCTGCGACGTTTGCAACTGCCTGGTAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTAcattcatt                                                       <  2:215768/151‑1 (MQ=255)

                                                                                  ccAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACattcattc                                                      <  2:69156/151‑1 (MQ=255)

                                                                                    aaTTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTcc                                                     <  1:318981/150‑1 (MQ=255)

                                                                                        ggCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTa                                                 <  2:433448/150‑1 (MQ=255)

                                                                                           aaTACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCg                                              >  1:178339/1‑150 (MQ=255)

                                                                                              aCCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCgaga                                           >  1:391521/1‑150 (MQ=255)

                                                                                                     agGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTc                                   <  2:481578/151‑1 (MQ=255)

                                                                                                       gAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCa                                  <  1:84454/150‑1 (MQ=255)

                                                                                                        aaTCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCAcg                                <  1:44875/151‑1 (MQ=255)

                                                                                                                ccACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCa                            >  2:1042/1‑147 (MQ=255)

                                                                                                                ccACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCa                            <  1:1042/147‑1 (MQ=255)

                                                                                                                   ccGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTgg                     <  2:403058/151‑1 (MQ=255)

                                                                                                                   ccGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTg                      <  1:554679/150‑1 (MQ=255)

                                                                                                                    cGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGATTTCTGCGACGTTTGCAACTGCCTTGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGtttg                     >  1:199521/1‑148 (MQ=255)

                                                                                                                           ccccGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCa             <  2:560527/151‑1 (MQ=255)

                                                                                                                                     aTAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTa   <  1:594534/151‑1 (MQ=255)

                                                                                                                                      tAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTCGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTAc  >  1:333093/1‑151 (MQ=255)

GCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCATCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTAC  >  NC_000913/2459984‑2460268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A