Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,533,049	C→T	A214V (GCG→GTG)	cysK →	cysteine synthase A, O‑acetylserine sulfhydrolase A subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,533,049	0	C	T	91.4%	115.4 / ‑3.1	35	A214V (GCG→GTG)	cysK	cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (7/25); minor base G (3/0); total (10/25)
Fisher's exact test for biased strand distribution p-value = 1.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGCGATTTCTACCGCGCGTCGTCT  >  NC_000913/2532907‑2533193

ggTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCGGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCa                                                                                                                                           <  2:621284/150‑1 (MQ=255)

ggTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAg                                                                                                                                          <  2:279924/151‑1 (MQ=255)

   cAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGt                                                                                                                                        <  1:350555/150‑1 (MQ=255)

     ggTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTAAGCTGACTTGCGGCAGACGCCAAATTTAAAGGACACAAAGCAAGACCCAAATTATTTCCGTCTCCGCTTGGGCCAACC‑ATTTTCCCCGTATTGGCCACGGGCTGGCAgggg                                                                                                                                       >  2:92931/1‑147 (MQ=255)

            gTATTTATTGCTGGCGTTGGGACTGGCGGTACGCCGGCTGGCGGCAGCCGCTACATTAAAAGCACCAAAAGCAAAAACGATCTTTTTTCTTTCGCCG‑TTTAGCCAACCCATTCTCCAGTTATTTCCCCGGGGCTGGCAGGGGAAGAGAtt                                                                                                                               >  2:143515/1‑150 (MQ=255)

                 tattGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAAcc                                                                                                                          <  2:53/150‑1 (MQ=255)

                  attGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCt                                                                                                                         <  2:573977/150‑1 (MQ=255)

                     gCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGcc                                                                                                                     <  2:23002/151‑1 (MQ=255)

                      cTGGCGTTGGGACTGGCGGTACGCTGACTCGGGGCTGCCGCTTCATTTAAAGGACCAAAAGGAAGAAAGGGCTTATTTTTTTCGCCG‑GTGGGCGCAACGATTCTTCAGGTATTGCCCACGGGCTGGGAGGGGGAAAGAGTTAAACTGGccc                                                                                                                    >  1:383222/1‑151 (MQ=255)

                              gggACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATaaaa                                                                                                            <  2:404283/151‑1 (MQ=255)

                              gggACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATaaa                                                                                                             <  1:534104/150‑1 (MQ=255)

                                    ggCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCa                                                                                                        <  1:638138/149‑1 (MQ=255)

                                    ggCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAgg                                                                                                      >  1:521968/1‑151 (MQ=255)

                                          aCGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTAtt                                                                                                 <  2:544501/150‑1 (MQ=255)

                                                   ggCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGt                                                                                        <  1:240115/150‑1 (MQ=255)

                                                                    tAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCt                                                                       <  2:60828/150‑1 (MQ=255)

                                                                     aaaGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCACAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCg                                                                     <  1:526797/151‑1 (MQ=255)

                                                                           aCCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGAtctc                                                                >  1:228586/1‑150 (MQ=255)

                                                                            ccAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGAtctc                                                                <  1:237479/149‑1 (MQ=255)

                                                                             cAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAg                                                             <  2:333778/151‑1 (MQ=255)

                                                                                aGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTg                                                          >  1:499274/1‑151 (MQ=255)

                                                                                     aGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGa                                                     <  1:358753/151‑1 (MQ=255)

                                                                                          gATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCCCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATaa                                                  >  1:576136/1‑149 (MQ=255)

                                                                                               tATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATaaa                                                 <  1:143511/145‑1 (MQ=255)

                                                                                                aTCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCAtt                                           <  2:41286/150‑1 (MQ=255)

                                                                                                       tCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCAc                                   >  2:526327/1‑151 (MQ=255)

                                                                                                        cGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCAc                                   <  2:304125/150‑1 (MQ=255)

                                                                                                            g‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATg                              <  1:130619/151‑1 (MQ=255)

                                                                                                              ttGAGCGTAACGATTCTCCAGTTATCGCCCATGTGGTTGCAGGTGAAGAGATTAAACCTGGACCGCATAAAATTCAGGGTAGTGGCGCTGCTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCTCCaa                                <  1:649775/148‑1 (MQ=255)

                                                                                                               tGAGCCAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATga                             >  1:21269/1‑150 (MQ=255)

                                                                                                                    cAACCGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGc                       <  2:271315/151‑1 (MQ=255)

                                                                                                                       ccGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGCGAt                    <  2:29213/151‑1 (MQ=255)

                                                                                                                        cGATTCTCCAGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGCGAtt                   >  2:268590/1‑151 (MQ=255)

                                                                                                                                 aGTTATCGCCCAGGTGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGCGATTTCTACcgcg          <  2:58900/151‑1 (MQ=255)

                                                                                                                                         cccAGGTGCTGGCAGGTGAAGAGATTAATCCTGGCCCGCATAAAATTCAGGGTGTTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGCTATTTCTACCGCGCGTCGTCt  <  1:143031/151‑1 (MQ=255)

GGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCG‑TTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCTGGCAGGTGAAGAGATTAAACCTGGCCCGCATAAAATTCAGGGTATTGGCGCTGGTTTTATCCCGGCTAACCTCGATCTCAAGCTGGTCGATAAAGTCATTGGCATCACCAATGAAGAAGCGATTTCTACCGCGCGTCGTCT  >  NC_000913/2532907‑2533193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A