Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,187	C→T	intergenic (‑105/‑33)	trpL ← / → yciV	trp operon leader peptide/PHP domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,187	0	C	T	100.0%	125.9 / NA	34	intergenic (‑105/‑33)	trpL/yciV	trp operon leader peptide/PHP domain protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/14); total (20/14)

AAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGCGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGC  >  NC_000913/1323043‑1323333

aaGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCaaa                                                                                                                                               >  2:17158/1‑150 (MQ=255)

          ccaACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATcc                                                                                                                                    <  2:363844/151‑1 (MQ=255)

                      tACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTg                                                                                                                        >  2:488093/1‑151 (MQ=255)

                          aaaaTTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGcc                                                                                                                    >  2:559982/1‑151 (MQ=255)

                             aTTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTg                                                                                                                 <  2:462984/151‑1 (MQ=255)

                                                 ccTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGt                                                                                             >  1:148543/1‑151 (MQ=255)

                                                      ttACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGAttt                                                                                        >  1:185298/1‑151 (MQ=255)

                                                      ttACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGAtt                                                                                         <  2:444404/150‑1 (MQ=255)

                                                         cGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACg                                                                                     <  2:147758/151‑1 (MQ=255)

                                                             aaCTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCt                                                                                 <  1:175054/151‑1 (MQ=255)

                                                                ttGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGc                                                                               >  1:538277/1‑150 (MQ=255)

                                                                  gCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGcac                                                                             <  1:567015/150‑1 (MQ=255)

                                                                   cGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAg                                                                           <  1:253565/151‑1 (MQ=255)

                                                                            tAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCTAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACcac                                                                  >  2:27043/1‑151 (MQ=255)

                                                                              aCTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACcac                                                                  >  2:270558/1‑149 (MQ=255)

                                                                              aCTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACcac                                                                  >  1:4898/1‑149 (MQ=255)

                                                                                      gatgatTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGAt                                                        >  1:93621/1‑151 (MQ=255)

                                                                                           ttAATTGTCAACAGCTCATTTCAGAATATTTGCAAGCACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTg                                                   >  1:392837/1‑151 (MQ=255)

                                                                                                    aaCAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGcc                                          <  2:417489/151‑1 (MQ=255)

                                                                                                     aCAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATTTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGGCCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCa                                         <  1:98008/151‑1 (MQ=255)

                                                                                                     aCAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGc                                           >  1:132094/1‑149 (MQ=255)

                                                                                                           cATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGc                                    >  2:427159/1‑150 (MQ=255)

                                                                                                             tttCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCAtt                                 >  1:504104/1‑151 (MQ=255)

                                                                                                              ttCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCAtt                                 <  1:624549/150‑1 (MQ=255)

                                                                                                               tCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTg                                <  2:33185/150‑1 (MQ=255)

                                                                                                                  gAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGc                            >  2:563942/1‑151 (MQ=255)

                                                                                                                             aGAACCGATATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGAGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGt                  >  1:477032/1‑150 (MQ=255)

                                                                                                                               aaCCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCga               <  1:468570/151‑1 (MQ=255)

                                                                                                                               aaCCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCg                >  1:143210/1‑150 (MQ=255)

                                                                                                                                   gTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATg           >  1:127562/1‑151 (MQ=255)

                                                                                                                                   gTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATg           >  1:347849/1‑151 (MQ=255)

                                                                                                                                     tATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATgcg         >  1:363470/1‑151 (MQ=255)

                                                                                                                                         atgTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTc     <  1:652446/151‑1 (MQ=255)

                                                                                                                                             cGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGc  <  1:44565/150‑1 (MQ=255)

AAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGCGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGC  >  NC_000913/1323043‑1323333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A