Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,617	A→G	D307G (GAC→GGC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,617	0	A	G	100.0%	137.4 / NA	37	D307G (GAC→GGC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/19); total (18/19)

GCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCG  >  NC_000913/1756472‑1756763

gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAg                                                                                                                                               <  2:671330/151‑1 (MQ=255)

    agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGAt                                                                                                                                           >  1:638921/1‑151 (MQ=255)

      aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATg                                                                                                                                          >  1:286019/1‑150 (MQ=255)

       agaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCt                                                                                                                                        <  2:587994/151‑1 (MQ=255)

            atgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTg                                                                                                                                   >  1:460343/1‑151 (MQ=255)

             tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTgg                                                                                                                                  >  2:434054/1‑151 (MQ=255)

              gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGt                                                                                                                                 <  1:139561/151‑1 (MQ=255)

                 cGAAAAATGTATCCGTGCACGTAAATTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCAGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGa                                                                                                                               <  1:486704/150‑1 (MQ=255)

                 cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGaa                                                                                                                              <  1:22419/151‑1 (MQ=255)

                    aaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATcc                                                                                                                           >  2:155203/1‑151 (MQ=255)

                     aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCg                                                                                                                          <  2:680309/151‑1 (MQ=255)

                     aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCg                                                                                                                          >  2:640285/1‑151 (MQ=255)

                       aTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCa                                                                                                                        <  2:672586/151‑1 (MQ=255)

                          tATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCaaa                                                                                                                      >  2:543433/1‑150 (MQ=255)

                           aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCaaaa                                                                                                                     <  2:20742/150‑1 (MQ=255)

                           aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAg                                                                                                                    <  1:326166/151‑1 (MQ=255)

                                tGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTaa                                                                                                                >  2:188219/1‑150 (MQ=255)

                                       aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGc                                                                                                        <  2:342167/151‑1 (MQ=255)

                                       aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGc                                                                                                        <  2:410307/151‑1 (MQ=255)

                                             gTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGa                                                                                                    >  2:113216/1‑149 (MQ=255)

                                                      gCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCt                                                                                          <  1:421918/150‑1 (MQ=255)

                                                               aTGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCg                                                                                 >  2:328311/1‑150 (MQ=255)

                                                                   tGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGAcc                                                                              >  1:156879/1‑149 (MQ=255)

                                                                    ggATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGCAAATACCCGCTGGAAGCGGTTTCTATCATGACGAccct                                                                            >  1:298479/1‑148 (MQ=255)

                                                                       ttCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGc                                                                        >  1:303455/1‑151 (MQ=255)

                                                                                    aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgacc                                                           <  1:56861/151‑1 (MQ=255)

                                                                                    aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgacc                                                           <  2:373335/151‑1 (MQ=255)

                                                                                                   cgcgCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGcc                                            >  1:346371/1‑151 (MQ=255)

                                                                                                      gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtc                                         <  1:509378/151‑1 (MQ=255)

                                                                                                      gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtc                                         >  2:266007/1‑151 (MQ=255)

                                                                                                        aGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtctc                                       >  2:487459/1‑151 (MQ=255)

                                                                                                                        gCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGAc                        <  1:131735/150‑1 (MQ=255)

                                                                                                                                cATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGACTCGAGTTCAACAATGACAACCGTaaa               <  2:663127/151‑1 (MQ=255)

                                                                                                                                        aCGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAt       <  2:209717/151‑1 (MQ=255)

                                                                                                                                          ggTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTa     <  2:477478/151‑1 (MQ=255)

                                                                                                                                            tACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc   >  2:183997/1‑151 (MQ=255)

                                                                                                                                              cTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg  >  1:46040/1‑150 (MQ=255)

GCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCG  >  NC_000913/1756472‑1756763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A